Efficacy of Glucocorticoids in the Treatment of Retinal Detachment With Choroidal Detachment: Analysis by Proteomics DOI

Pingping Li,

Mengyao Han, Rui Zhang

et al.

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

ABSTRACT Purpose Glucocorticoids are widely used for their anti‐inflammatory properties, but specific molecular mechanisms in treating rhegmatogenous retinal detachment with choroidal (RRDCD) remain unclear. This study aims to identify key regulatory factors the vitreous humor of RRDCD patients and analyze protein changes after hormonal intervention. Methods Vitreous fluid samples were collected during surgery from (RRD, n = 40), non‐glucocorticoid treated (nT‐RRDCD, 35), glucocorticoid‐treated (T‐RRDCD, 32). Primary outcomes reattachment status best‐corrected visual acuity (BCVA) at 6 months postoperatively. Proteomic analysis was performed using data‐independent acquisition (DIA), differentially expressed proteins validated by parallel reaction monitoring (PRM) ELISA. Results Between RRD nT‐RRDCD, 203 identified, while 295 between nT‐RRDCD T‐RRDCD. These involved complement activation, immune response, blood coagulation, MAPK signaling. Apolipoprotein D (APOD) vitronectin (VTN) positively correlated postoperative BCVA. APOD, serum amyloid A‐4 (SAA4), ubiquitin‐conjugating enzyme E2 variant emerged as potential diagnostic biomarkers RRDCD. Conclusions development involves multiple factors. mitigate damage suppressing inflammation, regulating oxidative stress, promoting cell repair. APOD VTN correlate BCVA, SAA4, show promise

Language: Английский

Efficacy of Glucocorticoids in the Treatment of Retinal Detachment With Choroidal Detachment: Analysis by Proteomics DOI

Pingping Li,

Mengyao Han, Rui Zhang

et al.

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

ABSTRACT Purpose Glucocorticoids are widely used for their anti‐inflammatory properties, but specific molecular mechanisms in treating rhegmatogenous retinal detachment with choroidal (RRDCD) remain unclear. This study aims to identify key regulatory factors the vitreous humor of RRDCD patients and analyze protein changes after hormonal intervention. Methods Vitreous fluid samples were collected during surgery from (RRD, n = 40), non‐glucocorticoid treated (nT‐RRDCD, 35), glucocorticoid‐treated (T‐RRDCD, 32). Primary outcomes reattachment status best‐corrected visual acuity (BCVA) at 6 months postoperatively. Proteomic analysis was performed using data‐independent acquisition (DIA), differentially expressed proteins validated by parallel reaction monitoring (PRM) ELISA. Results Between RRD nT‐RRDCD, 203 identified, while 295 between nT‐RRDCD T‐RRDCD. These involved complement activation, immune response, blood coagulation, MAPK signaling. Apolipoprotein D (APOD) vitronectin (VTN) positively correlated postoperative BCVA. APOD, serum amyloid A‐4 (SAA4), ubiquitin‐conjugating enzyme E2 variant emerged as potential diagnostic biomarkers RRDCD. Conclusions development involves multiple factors. mitigate damage suppressing inflammation, regulating oxidative stress, promoting cell repair. APOD VTN correlate BCVA, SAA4, show promise

Language: Английский

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