Three-Dimensional Culture Systems in Neuroblastoma Research DOI Creative Commons
Piotr Jung, Adam J. Wolpaw

Organoids, Journal Year: 2025, Volume and Issue: 4(2), P. 10 - 10

Published: May 8, 2025

Basic and translational cancer biology research requires model systems that recapitulate the features of human tumors. While two-dimensional (2D) cell cultures have been foundational allowed critical advances, they lack organizational complexity, cellular interactions, extracellular matrix present in vivo. Mouse models thus remained gold standard for studying cancer. In addition to high cost low throughput, mouse can also suffer from reduced tumor heterogeneity species-specific differences. Three-dimensional (3D) culture emerged as a key intermediary between 2D lines models, with lower greater flexibility than more accurate representation microenvironment lines. neuroblastoma, an aggressive childhood cancer, 3D applied study drug responses, motility, tumor–matrix interactions. Recent advances include integration immune cells immunotherapy studies, mesenchymal stromal tumor–stroma bioprinted manipulate properties. This review examines use highlighting their advantages limitations while emphasizing potential bridge gaps vitro, preclinical, clinical applications. By improving our understanding neuroblastoma biology, hold promise advancing therapeutic strategies outcomes this

Language: Английский

Three-Dimensional Culture Systems in Neuroblastoma Research DOI Creative Commons
Piotr Jung, Adam J. Wolpaw

Organoids, Journal Year: 2025, Volume and Issue: 4(2), P. 10 - 10

Published: May 8, 2025

Basic and translational cancer biology research requires model systems that recapitulate the features of human tumors. While two-dimensional (2D) cell cultures have been foundational allowed critical advances, they lack organizational complexity, cellular interactions, extracellular matrix present in vivo. Mouse models thus remained gold standard for studying cancer. In addition to high cost low throughput, mouse can also suffer from reduced tumor heterogeneity species-specific differences. Three-dimensional (3D) culture emerged as a key intermediary between 2D lines models, with lower greater flexibility than more accurate representation microenvironment lines. neuroblastoma, an aggressive childhood cancer, 3D applied study drug responses, motility, tumor–matrix interactions. Recent advances include integration immune cells immunotherapy studies, mesenchymal stromal tumor–stroma bioprinted manipulate properties. This review examines use highlighting their advantages limitations while emphasizing potential bridge gaps vitro, preclinical, clinical applications. By improving our understanding neuroblastoma biology, hold promise advancing therapeutic strategies outcomes this

Language: Английский

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