Inhibition of RPA32 and Cytotoxic Effects of the Carnivorous Plant Sarracenia purpurea Root Extract in Non-Small-Cell Lung Cancer Cells DOI Creative Commons

Kuo-Ting Chang,

Yu‐Cheng Chen, Yi Lien

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1426 - 1426

Published: May 9, 2025

The carnivorous plant Sarracenia purpurea has been traditionally used in various ethnobotanical applications, including treatments for type 2 diabetes and tuberculosis-like symptoms. This study investigates the cytotoxic effects of S. root extract (Sp-R) on human non-small-cell lung cancer (NSCLC) cell lines, H1975, H838, A549, focusing its impact survival, apoptosis, proliferation, migration. Additionally, ability to inhibit single-stranded DNA-binding activity RPA32 (huRPA32), a key protein DNA replication, was evaluated. Extracts from different parts (leaf, stem, root) were prepared using solvents (water, methanol, ethanol, acetone) screened apoptosis-inducing potential chromatin condensation assay. Among these, acetone-extracted fraction (Sp-R-A) exhibited most potent pro-apoptotic effects. MTT assay demonstrated dose-dependent effect NSCLC cells, with IC50 values 33.74 μg/mL 60.79 66.52 A549. Migration clonogenic assays further revealed that Sp-R-A significantly inhibited migration colony formation manner. Moreover, enhanced apoptosis when combined EGFR inhibitor afatinib, suggesting synergistic effect. electrophoretic mobility shift confirmed huRPA32, an 13.6 μg/mL. AlphaFold structural prediction molecular docking studies indicated major bioactive compounds purpurea, α-amyrin, ursolic acid, betulinaldehyde, strongly interact domain potentially contributing inhibitory Overall, these findings suggest huRPA32 is target anticancer against highlighted, supporting investigation into therapeutic applications.

Language: Английский

Inhibition of RPA32 and Cytotoxic Effects of the Carnivorous Plant Sarracenia purpurea Root Extract in Non-Small-Cell Lung Cancer Cells DOI Creative Commons

Kuo-Ting Chang,

Yu‐Cheng Chen, Yi Lien

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1426 - 1426

Published: May 9, 2025

The carnivorous plant Sarracenia purpurea has been traditionally used in various ethnobotanical applications, including treatments for type 2 diabetes and tuberculosis-like symptoms. This study investigates the cytotoxic effects of S. root extract (Sp-R) on human non-small-cell lung cancer (NSCLC) cell lines, H1975, H838, A549, focusing its impact survival, apoptosis, proliferation, migration. Additionally, ability to inhibit single-stranded DNA-binding activity RPA32 (huRPA32), a key protein DNA replication, was evaluated. Extracts from different parts (leaf, stem, root) were prepared using solvents (water, methanol, ethanol, acetone) screened apoptosis-inducing potential chromatin condensation assay. Among these, acetone-extracted fraction (Sp-R-A) exhibited most potent pro-apoptotic effects. MTT assay demonstrated dose-dependent effect NSCLC cells, with IC50 values 33.74 μg/mL 60.79 66.52 A549. Migration clonogenic assays further revealed that Sp-R-A significantly inhibited migration colony formation manner. Moreover, enhanced apoptosis when combined EGFR inhibitor afatinib, suggesting synergistic effect. electrophoretic mobility shift confirmed huRPA32, an 13.6 μg/mL. AlphaFold structural prediction molecular docking studies indicated major bioactive compounds purpurea, α-amyrin, ursolic acid, betulinaldehyde, strongly interact domain potentially contributing inhibitory Overall, these findings suggest huRPA32 is target anticancer against highlighted, supporting investigation into therapeutic applications.

Language: Английский

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