The mechanism of egg production improvement in laying hens before and after molting revealed by transcriptome and metabolome integration
Mengqing Sun,
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Hailing Wang,
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Xinyu Zhu
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et al.
Poultry Science,
Journal Year:
2025,
Volume and Issue:
unknown, P. 105125 - 105125
Published: April 1, 2025
The
objective
of
this
research
was
to
examine
the
effects
and
underlying
mechanisms
forced
molting
on
laying
rate
hens.
A
total
ninety
500-day-old
hens
were
randomly
assigned
three
groups:
a
control
group
(CK),
starvation
(SG),
recovery
(RG).
study
evaluated
follicular
development
in
measured
expression
levels
antioxidant,
lipid,
inflammatory
factors
their
serum.
Additionally,
transcriptomic
metabolomic
analyses
performed
assess
gene
metabolic
profiles
findings
indicated
that
led
an
increase
rates,
reduction
closure,
significant
rise
antioxidant
enzymes
such
as
GSH,
CAT,
SOD,
alongside
decrease
MDA
levels.
Furthermore,
there
reductions
blood
lipid
LDL,
HDL,
TC,
TG.
notable
differences
markers
TNF-α,
IL-1,
IL-6.
data
revealed
influenced
activation
PI3K-AKT
mTOR
signaling
pathways,
affecting
fatty
acid
metabolism
modulating
associated
genes.
In
conclusion,
demonstrates
is
effective
strategy
for
enhancing
it
provides
valuable
theoretical
framework
advancing
breeding
practices
aimed
at
improving
egg
production.
Language: Английский
Naringenin Targets PI3K p85alpha to Suppress PI3K/AKT Signaling Pathway and Ameliorate Disordered MMP-9 Secretion in Cigarette Smoke Extract-Induced Alveolar Macrophages In Vitro
Weiyang Fan,
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Ziyan Xu,
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Mengli Zhong
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et al.
Cells,
Journal Year:
2025,
Volume and Issue:
14(10), P. 678 - 678
Published: May 8, 2025
Background:
Naringenin
has
demonstrated
potential
therapeutic
effects
against
cigarette
smoke-induced
lung
injury;
however,
its
underlying
mechanisms
of
regulating
matrix
metalloproteinase-9
(MMP-9)
in
alveolar
macrophages
remain
unclear.
Methods:
The
regulatory
naringenin
smoke
extract
(CSE)-induced
were
investigated
using
proteomics,
and
then,
naringenin’s
targets
further
validated
by
Western
blot,
molecular
docking,
dynamics
(MD)
simulations,
cellular
thermal
shift
assay
(CETSA),
enzyme
activity
assay.
Results:
proteomics
revealed
that
the
PI3K/AKT
signaling
pathway
might
play
a
crucial
role
inhibition
MMP-9.
blot
analysis
confirmed
significantly
inhibited
CSE-upregulated
reduced
MMP-9
expression
MH-S
cells.
Notably,
PI3K
activator
740Y-P
reversed
on
Additionally,
MD
CETSA
identified
p85alpha
as
binding
site
for
naringenin,
markedly
CSE-induced
activity.
In
vitro
experiments,
inhibiting
secretion
contributed
to
alleviating
elastin
E-cadherin
damage
epithelial
Furthermore,
effectively
suppressed
primary
mouse
human
THP-1-differentiated
macrophages.
Conclusions:
Our
findings
candidate
treating
smoking-induced
injury,
directly
targeted
p85alpha,
via
suppressing
pathway.
Language: Английский