Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 578 - 578
Published: May 11, 2025
Most DNA damage caused by oxidative metabolism consists of single lesions that can accumulate in tissues. This review focuses on two classes lesions: the 8-oxopurine (8-oxo-Pu) are repaired base excision repair (BER) enzyme and four 5',8-cyclopurine (cPu) exclusively nucleotide (NER) enzyme. The aim is to correlate simultaneous quantification these context neurological disorders. first half a summary reactive oxygen species (ROS) with particular attention pathways hydroxyl radical (HO•) formation, followed protocols for six biomimetic chemistry HO• double-stranded oligonucleotides (ds-ODN) calf thymus (ct-DNA). second addresses neurodegenerative diseases: xeroderma pigmentosum (XP) Cockayne syndrome (CS). quantitative data obtained from genomic and/or mitochondrial extracts across several XP CS cell lines discussed. Oxidative stress contributes resulting accumulation cPu 8-oxo-Pu DNA. formation postulated culprit inducing symptoms associated CS.
Language: Английский