Expression of minichromosome maintenance proteins in the exfoliated cells supplement sputum cytology in the diagnosis of lung cancer DOI Open Access

Neeraja Panakkal,

Asha Lekshmi,

K. M. Jagathnath Krishna

et al.

CytoJournal, Journal Year: 2024, Volume and Issue: 21, P. 81 - 81

Published: Dec. 31, 2024

Objective: Sputum cytology is recognized as a straightforward and noninvasive way to diagnose lung cancer, although its clinical utility has not yet been investigated. The objective of the study was detect classify cancerous cells in sputum by examining their expression minichromosome maintenance proteins (MCM2 MCM7). In addition, attempted evaluate these proteins’ potential biomarkers cancer lesions relationships with clinicopathological characteristics. Material Methods: MCM2 MCM7 samples evaluated using immunocytochemistry cell blocks ( n = 97), correlation features examined. Diagnostic performance function sensitivity specificity. Results: Immunoexpression confined nuclei malignant alone, suggesting differential diagnostic marker. They showed significant correlations tumor P < 0.001), while alone exhibited stage 0.014). overexpression markers notably pronounced adenocarcinoma compared other subtypes. terms characterizing cells, protein demonstrated highest at 92% an area under curve (AUC) 0.961, whereas had 80% AUC 0.901. Conclusion: This presents inaugural use on exfoliated samples, proposing that analyzing immunocytochemical could serve cost-effective approach for diagnosing cancer. Integrating assessed into routine cytopathology laboratories augment traditional morphological evaluations, thereby improving cytology.

Language: Английский

The Power of Antibodies: Advancing Biomarker-Based Disease Detection and Surveillance DOI
Woei Kean Ng,

Gunasegran Thanusha,

Pei Pei Chong

et al.

Immunological Investigations, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: April 21, 2025

Antibodies have long served as fundamental tools in disease diagnosis and surveillance. Their utility biomarkers has expanded beyond infectious diseases to encompass a wide range of health conditions. This review aims explore recent advancements antibody biomarker discovery their applications diagnosing monitoring diverse It also examines the role surveillance public epidemiological studies. A comprehensive analysis literature was conducted, focusing on studies that identify characterize disease-specific antibodies. Particular attention given relevance autoimmune diseases, infections, cancers, neurological disorders. The highlights clinical significance. discusses challenges antibody-based assessing prevalence, tracking immunity trends, supporting One Health strategies. Recent demonstrate significant potential improving early diagnosis, personalized treatment, population-level management. Antibody continues play pivotal guiding responses understanding dynamics.

Language: Английский

Citations

0
Expression of minichromosome maintenance proteins in the exfoliated cells supplement sputum cytology in the diagnosis of lung cancer DOI Open Access

Neeraja Panakkal,

Asha Lekshmi,

K. M. Jagathnath Krishna

et al.

CytoJournal, Journal Year: 2024, Volume and Issue: 21, P. 81 - 81

Published: Dec. 31, 2024

Objective: Sputum cytology is recognized as a straightforward and noninvasive way to diagnose lung cancer, although its clinical utility has not yet been investigated. The objective of the study was detect classify cancerous cells in sputum by examining their expression minichromosome maintenance proteins (MCM2 MCM7). In addition, attempted evaluate these proteins’ potential biomarkers cancer lesions relationships with clinicopathological characteristics. Material Methods: MCM2 MCM7 samples evaluated using immunocytochemistry cell blocks ( n = 97), correlation features examined. Diagnostic performance function sensitivity specificity. Results: Immunoexpression confined nuclei malignant alone, suggesting differential diagnostic marker. They showed significant correlations tumor P < 0.001), while alone exhibited stage 0.014). overexpression markers notably pronounced adenocarcinoma compared other subtypes. terms characterizing cells, protein demonstrated highest at 92% an area under curve (AUC) 0.961, whereas had 80% AUC 0.901. Conclusion: This presents inaugural use on exfoliated samples, proposing that analyzing immunocytochemical could serve cost-effective approach for diagnosing cancer. Integrating assessed into routine cytopathology laboratories augment traditional morphological evaluations, thereby improving cytology.

Language: Английский

Citations

0