RNA interference in Pardosa pseudoannulata, an important predatory enemy against several insect pests, through ingestion of dsRNA‐expressing Escherichia coli DOI
Nannan Hou, Zhenlei Zhou, Yimeng Chen

et al.

Insect Molecular Biology, Journal Year: 2021, Volume and Issue: 30(6), P. 624 - 631

Published: Aug. 19, 2021

RNA interference is an important technology for gene functional research in many organisms. The pond wolf spider (Pardosa pseudoannulata) natural enemy of rice field pests. To facilitate large-scale this species and others, we developed (RNAi) method via ingestion bacteria expressing dsRNA. dsRNA targeting a cytochrome P450 monooxygenase (cyp41g2) was expressed Escherichia coli HT115 (DE3). And then the bacterial suspension fed to 14-20 days old spiderlings. mRNA abundance target significantly reduced after 3-day's dsRNA, between day 5 7, RNAi efficiency remained stable. Thus, selected as optimum time. Furthermore, resuspension containing 20 ng/μl concentration. evaluate applicability method, three other genes with different tissue expression pattern were also targets. all four 66.0% up 86.9%. results demonstrated that oral delivery would be effective study P. pseudoannulata.

Language: Английский

Emerald Ash Borer Management and Research: Decades of Damage and Still Expanding DOI
Jianghua Sun, Tuuli‐Marjaana Koski, Jacob D. Wickham

et al.

Annual Review of Entomology, Journal Year: 2023, Volume and Issue: 69(1), P. 239 - 258

Published: Sept. 14, 2023

Since the discovery of ash tree ( Fraxinus spp.) killer emerald borer (EAB; Agrilus planipennis) in United States 2002 and Moscow, Russia 2003, substantial detection management efforts have been applied to contain monitor its spread mitigate impacts. Despite these efforts, pest continues within North America. It has European Ukraine is causing sporadic outbreaks native range China. The dynamics EAB's expansion events appear be linked lack resistant trees invaded ranges, facilitated by abundance or planted American susceptible species. We review recently gained knowledge EAB; ecological, economic, social impacts; past with their successes limitations. also highlight advances biological control, mechanisms resistance, new approaches under development, aim guiding more effective management.

Language: Английский

Citations

27
RNAi-based biocontrol for crops: a revised expectation for a non-recent technology DOI
Popi Septiani, Yonadita Pramesti,

Muhammad Ghildan

et al.

Planta, Journal Year: 2025, Volume and Issue: 261(2)

Published: Jan. 25, 2025

Language: Английский

Citations

1
Advances in the Development of Microbial Double-Stranded RNA Production Systems for Application of RNA Interference in Agricultural Pest Control DOI Creative Commons
Ruobing Guan,

Dongdong Chu,

Xinyi Han

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 9

Published: Sept. 13, 2021

RNA interference (RNAi) is a valuable and revolutionary technology that has been widely applied in medicine agriculture. The application of RNAi various industries requires large amounts low-cost double-stranded (dsRNA). Chemical synthesis can only produce short dsRNAs; long dsRNAs need to be synthesized biologically. Several microbial chassis cells, such as Escherichia coli, Saccharomyces cerevisiae, Bacillus species, have used for dsRNA synthesis. However, the titer, rate production, yield obtained by these microorganism-based strategies still low. In this review, we summarize advances analyze merits faults different production systems. This review provides guide system selection. Future development efficient systems also discussed.

Language: Английский

Citations

52
RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype? DOI Creative Commons

Mallikarjuna Reddy Joga,

Kanakachari Mogilicherla, Guy Smagghe

et al.

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Sept. 10, 2021

Graphical Abstract Three-tier impact of forest protection products (FPPs).

Language: Английский

Citations

43
Functional characterization of tyrosine melanin genes in the white-backed planthopper and utilization of a spray-based nanoparticle-wrapped dsRNA technique for pest control DOI
Huan Guo,

Gui‐Jun Long,

Xuan-Zheng Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 230, P. 123123 - 123123

Published: Jan. 2, 2023

Language: Английский

Citations

23
Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities DOI Creative Commons
Kiran Singewar, Matthias Fladung

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(2)

Published: May 27, 2023

Abstract Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift their hosts, resulting in novel outbreaks that damage forests ecology. Traditional management schemes are unable control such outbreaks, therefore unconventional competitive governance is needed manage forest RNA interference (RNAi) mediated double-stranded (dsRNA) treatment method can be implemented protect trees. Exogenous dsRNA triggers RNAi-mediated gene silencing of a vital gene, suspends protein production, death targeted pathogens pests. successful for many crop insects fungi, however, studies against depleting. Pesticides fungicides based on could used combat caused different parts world. Although has proved its potential, crucial dilemma risks including species-specific selection, delivery methods cannot overlooked. Here, we summarized major fungi insect have genomic information, fungi-and pesticides. Current challenges opportunities target decision, using nanoparticles, direct applications, new mycorrhiza tree protection discussed. importance affordable next-generation sequencing minimize impact non-target species We suggest collaborative research among genomics pathology institutes develop necessary strategies species.

Language: Английский

Citations

22
RNAi-Based Biopesticides Against 28-Spotted Ladybeetle Henosepilachna vigintioctopunctata Does Not Harm the Insect Predator Propylea japonica DOI
Shimin Chen,

Xuming Luo,

Satyabrata Nanda

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(7), P. 3373 - 3384

Published: Feb. 10, 2023

RNA interference (RNAi)-mediated control of the notorious pest Henosepilachna vigintioctopunctata is an emerging environment friendly research area. However, characterization key target genes in H. crucial for this. Additionally, assessing risk RNAi to nontarget organisms (NTOs) necessary environmental safety. In this study, potential technology controlling infestation has been investigated by oral delivery double-stranded (dsRNA). The results revealed that silencing six genes, including HvABCH1, HvHel25E, HvProsbeta5, HvProsalpha6, HvProsbeta6, and HvSrp54k, was highly lethal vigintioctopunctata. LC50 values dsRNAs used silence these were found be less than 13 ng/μL. Moreover, use bacterially expressed caused high mortality lab field populations Further, administration HvHel25E HvSrp54k predatory lady beetle Propylea japonica confirmed no transcriptional or organismal levels effects. This risk-assessment result ensured off-target effects on NTOs. Overall, findings study suggested can novel promising molecular targets with specificity management negligible

Language: Английский

Citations

20
Highly Variable Dietary RNAi Sensitivity Among Coleoptera DOI Creative Commons
Jonathan Willow, Eve Veromann

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 7, 2021

Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and forest trees, plant biotechnology solutions are being developed with the hope of limiting these losses. Due unprecedented target-specificity double-stranded RNA (dsRNA), its utility inducing interference (RNAi) when consumed by target pest species, dsRNA-based approaches represent cutting edge current pesticide research development. We review dietary RNAi studies coleopterans discuss prospects future directions regarding RNAi-based management coleopteran pests. Herein, we also provide a balanced overview existing order an accurate re-assessment sensitivity coleopterans, despite limitations body scientific literature. further impediments our understanding this important insect identify critical for area, emphasis on using approaches.

Language: Английский

Citations

33
RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture DOI Creative Commons
Shuhei Hashiro, Hisashi Yasueda

Applied Sciences, Journal Year: 2022, Volume and Issue: 12(6), P. 2954 - 2954

Published: March 14, 2022

RNA interference (RNAi)-based pesticides are pest control agents that use RNAi mechanisms as the basis of their action. They regarded environmentally friendly and a promising alternative to conventional chemical pesticides. The effective substance in RNAi-based is double-stranded (dsRNA) designed match nucleotide sequence target essential gene concern. When taken up by pest, this exerts an effect inhibits some vital biochemical/biological process pest. dsRNA products also expected be applied for viral diseases aquaculture RNAi, especially shrimp farming. A critical issue practical application production must low-cost. Here, we review recent methods microbial dsRNAs using representative microorganisms (Escherichia coli, Pseudomonas syringae, Corynebacterium glutamicum, Chlamydomonas reinhardtii, others) host strains. characteristics each system discussed.

Language: Английский

Citations

25
Evolution and Adaptation of Forest and Crop Pathogens in the Anthropocene DOI Creative Commons

Pauline Hessenauer,

Nicolas Feau, Upinder Gill

et al.

Phytopathology, Journal Year: 2020, Volume and Issue: 111(1), P. 49 - 67

Published: Nov. 17, 2020

Anthropocene marks the era when human activity is making a significant impact on earth, its ecological and biogeographical systems. The domestication intensification of agricultural forest production systems have had large plant tree health. Some pathogens benefitted from these activities evolved adapted in response to expansion crop systems, resulting global outbreaks. Global pathogen genomics data including population high-quality reference assemblies are crucial for understanding evolution adaptation pathogens. Crops trees remarkably different characteristics, such as reproductive time level domestication. They also disease management with more intensive crops than trees. By comparing contrasting results genomic studies done widely we can improve our selection pressures. We find that spite differences, similar processes hybridization, host jumps, selection, specialization, clonal shaping populations both propose some solutions reduce impacts lower probability outbreaks so envision better strategies sustain food well ecosystem services.

Language: Английский

Citations

33