Episyrphus balteatus symbiont variation across developmental stages, living states, two sexes, and potential horizontal transmission from prey or environment

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 5, 2024

Introduction Episyrphus balteatus is one representative Syrphidae insect which can provide extensive pollination and pest control services. To date, the symbiont composition potential acquisition approaches in remain unclear. Methods Herein, we investigated microbiota dynamics across developmental stages, different living states, two sexes E. via full-length 16S rRNA genes sequencing, followed by an attempt to explore possibility of transmission from prey Megoura crassicauda hoverfly. Results Overall, Proteobacteria Firmicutes were dominant bacteria phyla with fluctuating relative abundances life stage. Cosenzaea myxofaciens adulthood, while Enterococcus silesiacus Morganella morganii dominate larvae pupae , respectively. Unexpectedly, Serratia symbiotica facultative endosymbiont commonly harbored aphids, was predominant just behind . In addition, S. also surprisingly most dominated M. aphids (92.1% abundance), are significantly higher than Buchnera aphidicola (4.7% primary obligate aphid species. Approximately 25% mortality observed among newly emerged adults, disordered, similar normally dying individuals. Sexually biased symbionts 41 species pairwise co-occurrence 23 biomarker for each group identified eventually. Functional prediction showed hoverflies both mainly focusing on metabolic pathways. brief, comprehensively explored microbiome hoverfly using reared indoors as model, revealed its species, sexually symbionts, found inhabited We that symbiotic other Discussion Taken together, this study provides new valuable resources about jointly, will benefit further exploring roles

Language: Английский

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Böceklerle İlişkili $Micrococcus$ sp. Türlerinin Moleküler Karakterizasyonu ve $Galleria$ $mellonella$ (Lepidoptera: Pyralidae)’ya Karşı Virülansları

Sinop Üniversitesi Fen Bilimleri Dergisi, Journal Year: 2024, Volume and Issue: 9(1), P. 14 - 25

Published: Feb. 22, 2024

Şimdiye kadar 100’den fazla bakteri türünün eklem bacaklılarda hastalık oluşturduğu bilinmektedir. Entomopatojenik bakteriler ucuz olmaları, kitle üretimindeki kolaylık, konak spesifikliği, güvenlik ve çevrede kalıcılık gibi nedenlerden ötürü zararlı böceklerle mikrobiyal mücadelede uzun yıllardan beri kullanılmaktadır. Bu çalışmada çeşitli böcek örneklerinden ($Malacosoma$ sp. (Lepidoptera: Lasiocampidae), $Ogcodocera$ (Diptera: Bombyliidae) $Orgyia$ Erebidae)) izole edilen altı (6) adet suşu ilk etapta morfolojik olarak $Micrococcus$ tanımlanmıştır. Daha sonra bu suşlarının 16S rRNA sekans analizi ile moleküler seviyede tanımlanmaları gerçekleştirilmiştir. Ayrıca $Galleria$ $mellonella$ Pyralidae) larvalarına karşı öldürücülük etkileri belirlenmiştir. Altı da (MK-5, AS-2, AS-3, AS-4, BB-1 BB-5) cins düzeyinde $G.$ patojenite testleri sonucunda ise sadece MK-5 %70 ölüm oranına neden olmuş diğer suşlar önemli derecede olmamıştır. Elde sonuçların ilişkili simbiyotik bakterilerin tanımlanmasında patojenik özelliklerinin belirlenmesinde faydalı olacağı düşünülmektedir.

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Insect Gut Microbiota and Pesticide Degradation in Response to Innate Metabolites- a Review

Indian Journal of Entomology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 12

Published: Jan. 23, 2024

Insects host a diverse microbiota in their gut, encompassing bacteria, fungi, viruses, and archaea, influencing physiology, nutrition, overall health. The composition of these microbial communities varies with factors like insect species, diet, the environment. Insect gut serve pivotal roles such as aiding digestion, synthesizing essential nutrients, safeguarding against pathogens, detoxifying toxins, including insecticides. A particularly promising facet function lies metabolism These microbiotas can either augment or diminish insecticide toxicity through mechanisms enzymatic breakdown, sequestration, target site alteration, modulation insect's immune response. Understanding interactions is paramount for devising sustainable pest management strategies. This review explores into microbiota, impact on susceptibility, potential use metabolites eco-friendly control. We explore pesticide degradation mechanisms, consequences disruption role microbiota-produced shaping efficacy. Ultimately, we highlight manipulation strategy to enhance effectiveness combat resistance management.

Language: Английский

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Understanding Insect-microbe Interactions for Sustainable Pest Management

Indian Journal of Entomology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: Jan. 22, 2024

Due to their enormous diversity, small body weight, flight and shorter lifecycles, insects thrive in almost all macro- microhabitats. A plethora of microorganisms interact with as symbionts. Insect-microbial symbiont interaction can be either mutualistic, commensalism or pathogenic. The entry a microbe mounts immune defenses the insect at local systemic level order mitigate damage inflicted. Microbes, on other hand, synthesize various molecules/ toxins develop strategies evade counter defenses, allowing them utilize host resources for reproduction, coexistence transmission. This review provides comprehensive understanding these insect-microbe interactions including bacteria, fungi virus. Attempt has also been made highlight tripartite between insects, microbes plants view need sustainable pest management

Language: Английский

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Gregarines impact consumption and development but not glucosinolate metabolism in the mustard leaf beetle

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: May 1, 2024

Gregarines are usually classified as parasites, but recent studies suggest that they should be viewed on a parasitism-mutualism spectrum and may even seen part of the gut microbiota host insects. As such, also impact consumption their hosts and/or involved in digestion or detoxification host’s diet. To study such effects gregarine species those traits its host, mustard leaf beetle ( Phaedon cochleariae ) was used. This feeds Brassicaceae plants contain glucosinolates, which form toxic compounds when hydrolyzed by myrosinases. We cleaned eggs from gametocysts spores reinfected half larvae with gregarines, to obtain gregarine-free (G-) gregarine-infected (G+) larvae. Growth food parameters these were assessed rearing individuals watercress Nasturtium officinale , Brassicaceae). A potential involvement gregarines glucosinolate metabolism P. investigated offering G- G+ discs (containing mainly benzenic 2-phenylethyl myrosinases) pea Pisum sativum Fabaceae, lacking glucosinolates treated aliphatic 4-pentenyl indole 1-methoxy-3-indolylmethyl glucosinolate. Larval fecal samples analyzed via UHPLC-QTOF-MS/MS search for breakdown metabolites. development, body mass, growth rate efficiency convert into mass negatively affected infection while pupal remained unaffected. The metabolites conjugated aspartic acid, glutamic acid. Gregarine did not alter larvae’s ability metabolize independent plant In summary, some negative performance could shown, indicating parasitism. Future further disentangle this gregarine-host relationship investigate microbiome potentially metabolism.

Language: Английский

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