Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(2), P. 264 - 264
Published: Jan. 26, 2024
Metarhizium
rileyi
is
an
entomopathogenic
fungus
that
naturally
infects
the
larvae
of
Spodoptera
frugiperda,
and
has
biocontrol
potential.
To
explore
more
natural
fungi
resources,
a
total
31
strains
were
isolated
from
13
prefectures
in
Yunnan
Province.
All
identified
using
morphology
molecular
biology.
The
genetic
diversity
isolates
M.
was
analyzed
inter-simple
sequence
repeat
(ISSR)
techniques.
Seven
primers
with
good
polymorphism
selected,
fifty-four
distinct
amplification
sites
obtained
by
polymerase
chain
reaction
amplification.
Among
them,
50
polymorphic
sites,
percentage
94.44%.
thirty-one
divided
into
eight
subpopulations
according
to
regions.
Nei’s
gene
0.2945,
Shannon
information
index
0.4574,
indicating
had
rich
diversity.
average
different
regions
0.2962,
within
populations
0.1931,
differentiation
coefficient
0.3482
(>0.25),
flow
0.9360
(<1).
individual
cluster
analysis
showed
there
no
obvious
correlation
between
their
geographical
origin,
which
also
indicated
virulence
not
related
phylogeny.
Thus,
distance
Province
distance.
those
32
against
3rd-instar
S.
frugiperda
varied
differences
locations.
On
10th
day
inoculation,
seventeen
insect
mortality
rate
70.0%,
seven
100%.
half-lethal
times
SZCY201010,
XSBN200920,
MDXZ200803
less
than
4
d.
they
have
potential
be
developed
fungal
insecticidal
agents.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(9), P. 4156 - 4156
Published: April 27, 2025
Insects
are
among
the
most
diverse
and
abundant
organisms
on
Earth,
their
population
dynamics
strongly
influenced
by
entomopathogenic
fungi.
This
study
examines
role
of
carbon
nitrogen
metabolism
in
virulence
fungus
Metarhizium
rileyi
against
migratory
locust,
Locusta
migratoria.
The
findings
demonstrate
that
capacity
M.
to
utilize
different
sources
is
a
key
factor
its
virulence.
Specifically,
two
strains
(PPDB201006
SZCY201010)
exhibited
distinct
metabolic
abilities,
with
PPDB201006
displaying
superior
growth
enzyme
activities
various
compared
SZCY201010.
These
differences
were
associated
significant
variations
virulence,
as
induced
higher
mortality
rates
L.
migratoria
than
Metabolomics
analysis
revealed
infection
led
substantial
alterations
hemolymph
metabolites
migratoria,
particularly
organic
acids,
amino
sugars,
lipids.
results
emphasize
significance
pathogenicity
fungi
offer
new
perspectives
for
optimizing
application
biological
control
agents.
not
only
improves
our
understanding
fungal
mechanisms
but
also
contributes
development
more
effective
sustainable
pest
management
strategies.
Egyptian Journal of Biological Pest Control,
Journal Year:
2024,
Volume and Issue:
34(1)
Published: March 30, 2024
Abstract
Background
Metarhizium
rileyi
is
an
important
entomopathogenic
fungus
which
has
a
wide
range
of
host
pests.
In
this
work,
the
virulence
M.
EFNL-08
against
3rd
instar
larvae
fall
armyworm,
Spodoptera
frugiperda
(J.E.
Smith)
(Lepidoptera:
Noctuidae),
was
quantified.
Additionally,
infection
process
and
aspects
immunological
response
were
described
in
laboratory.
Results
Bioassay
results
proved
that
highly
virulent
to
S.
with
98.33%
mortality
at
1
×
10
8
conidia/ml.
Scanning
electron
microscopy
used
monitor
morphological
process.
When
hyphal
bodies
(Hbs)
injected
into
hemocoel
host,
they
not
recognized
by
host’s
immune
system
as
invaders.
There
nonsignificant
differences
hemocytes
phenoloxidase
activity
between
infected
control
initial
30
h,
indicating
successfully
avoids
attack
from
system.
However,
it
inhibited
enzyme
latest
stage
when
Hbs
reached
high
density.
After
,
glutathione-S-transferase
(GST)
presented
significant
upward
trend,
while
acetylcholinesterase
(AchE)
initially
increased,
followed
decrease,
eventually
stabilized.
both
detoxification
enzymes
group
showed
slight
fluctuations
stage,
then
stabilized
low
level.
Conclusion
Obtained
provided
new
insights
potential
physiological
responses
.
This
provides
ideas
for
further
development
novel
efficient
fungi
(EPF)
pest
control.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(6), P. 1129 - 1129
Published: May 31, 2024
Beauveria
bassiana
and
Metarhizium
rileyi
are
extensively
utilized
to
investigate
fungal
pathogenic
mechanisms
develop
biological
control
agents.
Notwithstanding,
notable
distinctions
exist
in
their
pathogenicity
against
the
same
host
insect.
This
study
aimed
elucidate
differences
between
M.
B.
by
examining
impact
of
various
ratios
strain
AJS91881
SXBN200920
on
fifth
instar
larvae
Spodoptera
litura,
focusing
early
infection
stages
intestinal
microbial
community
structure.
The
lethal
time
50
(LT50)
for
was
significantly
lower
than
that
rileyi,
indicating
greater
efficacy.
Survival
analyses
mixed
groups
(ratios
1:9,
1:1,
9:1
bassiana)
consistently
demonstrated
higher
virulence
bassiana.
Intestinal
diversity
analysis
revealed
a
significant
increase
Achromobacter
Pseudomonas
infected
with
whereas
Weissella
notably
those
Additionally,
shifts
genera
abundances
were
observed
across
all
groups.
KEGG
pathway
enrichment
indicated
employ
distinct
strategies
during
stages.
In
vitro
tests
confirmed
superior
growth
stress
resistance
compared
but
antifungal
ability
better
conclusion,
our
findings
provide
preliminary
insights
into
differential
behaviors
S.
litura
larvae,
enhancing
understanding
informing
pest
agriculture
forestry.
Communications Biology,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: July 3, 2024
Insecticide
resistance
has
been
a
problem
in
both
the
agricultural
pests
and
vectors.
Revealing
detoxification
mechanisms
may
help
to
better
manage
insect
pests.
Here,
we
showed
that
arylalkylamine
N-acetyltransferase
1
(AANAT1)
regulates
intestinal
process
through
modulation
of
reactive
oxygen
species
(ROS)-activated
transcription
factors
cap"n"collar
isoform-C
(CncC):
muscle
aponeurosis
fibromatosis
(Maf)
pathway
oriental
fruit
fly,
Bactrocera
dorsalis,
arbovirus
vector,
Aedes
aegypti.
Knockout/knockdown
AANAT1
led
accumulation
biogenic
amines,
which
induced
decreased
gut
ROS
level.
The
reduced
midgut
levels
resulted
expression
CncC
Maf,
leading
lower
level
genes.
knockout/knockdown
insects
were
more
susceptible
insecticide
treatments.
Our
study
reveals
normal
functionality
is
important
for
regulation
pathways,
providing
insights
into
mechanism
underlying
defense
against
xenobiotics
metazoans.
Insects,
Journal Year:
2025,
Volume and Issue:
16(2), P. 199 - 199
Published: Feb. 12, 2025
Spodoptera
frugiperda
is
a
major
migratory
agricultural
pest
that
poses
significant
threat
to
global
crop
safety.
Metarhizium
rileyi
has
emerged
as
an
effective
biocontrol
agent
against
lepidopteran
pests.
In
this
study,
we
examined
the
immune
responses
of
third-instar
S.
larvae
at
various
stages
M.
infection.
Using
RNA-seq
and
microscopic
observation,
identified
immune-related
pathways
enriched
different
infection
stages,
which
were
further
validated
by
qRT-PCR.
Our
findings
revealed
following
during
infection:
During
stage
when
penetrated
host
cuticle
(0-48
h),
genes
related
energy
metabolism,
detoxification,
melanization
upregulated.
Meanwhile,
TOLL
IMD
signaling
activated
counter
rileyi's
internal
(48-96
was
peak
expression
period
genes,
cellular
immunity
predominated.
Hemocytes
encapsulated
phagocytosed
hyphal
bodies.
Phagocytosis
enhanced
through
upregulation
ROS
melanization-related
well
involved
in
insect
hormone
biosynthesis.
grew
from
inside
outside
(96-120
system
paralysis
resulted
mortality.
These
deepen
our
understanding
interactions
between
frugiperda,
support
potential
agent,
provide
theoretical
foundation
for
development
targeted
biopesticides
pests
using
biotechnological
approaches.
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(8), P. 521 - 521
Published: July 26, 2024
is
a
wide
spread
insect
fungi
with
good
biocontrol
potentiality
to
various
pests,
particularly
noctuid
insects.
However,
it
characterized
by
its
slow
growth,
sensitivity
abiotic
stress,
and
the
speed
of
kill
which
hinder
use
compared
other
entomopathogenic
fungi.
In
this
study,
responses
Journal of Asia-Pacific Entomology,
Journal Year:
2023,
Volume and Issue:
26(4), P. 102152 - 102152
Published: Sept. 14, 2023
The
fall
armyworm,
Spodoptera
frugiperda
(Lepidoptera:
Noctuidae),
is
a
major
maize
pest
worldwide
and
has
caused
huge
economic
loss.
To
explore
the
control
tactics
of
S.
in
mountain
planting
fields,
studies
were
performed
China
from
2020
to
2021
for
screening
frugiperda-resistant
variety,
seed
coating
agents,
chemical
insecticides,
biological
agents.
results
showed
that
75
g/hm2
Emamectin
benzoate
had
better
controlling
efficacy
(with
91.05%
at
1d)
than
other
chemicals,
so
it
was
recommended
high
incidence
period
pests.
Compared
with
treatments,
50%
Lumingwei
TM625FS
76.19%
10d)
best
against
frugiperda.
"Telenomus
remus
+
Picromerus
lewisi"
"T.
Eocanthecona
furcellata"
good
on
76.01%,
64.10%
20d,
respectively.
Additionally,
there
lower
numbers
variety
GuiDan6,
BaoYu9,
YanBaiYu8.
In
summary,
this
research
four
effective
management
strategies
(GuiDan6-resistant
TM625FS-seed
5%
emamectin
benzoate-chemical
pesticide,
P.
lewisi"-biological
control)
fields.
study
provided
