Rad6, a ubiquitin conjugator required for insect-pathogenic lifestyle, UV damage repair, and genomic expression of Beauveria bassiana

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127622 - 127622

Published: Jan. 18, 2024

Language: Английский

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Emulsifiable oil‐formulated Beauveria bassiana competes with imidacloprid for seasonal control of cereal aphids in Zhejiang, China

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(6), P. 2929 - 2936

Published: Jan. 29, 2024

Abstract BACKGROUND Alternatives to neonicotinoids against cereal aphids are needed mitigate aphid resistance and non‐target effects. The emulsifiable oil formulations of two Beauveria bassiana strains, namely Bb registered as a mycoinsecticide TBb overexpressing an endogenous virulence factor, were tested for seasonal control at the elongating (April 7) milk ripening (May 12) stages winter wheat crop in Yuhang, Zhejiang. Each three field trials consisted blank treatments (three randomized 100‐m 2 plots per capita ) each fungal strain sprayed biweekly rates 1.0 × 10 13 1.5 conidia ha −1 10% imidacloprid WP label rate. RESULTS Tiller infestation percentage density 5‐week after first spray reduced 18.7–22.4% 9.1–12.4 tiller treatments, 12.8–25.3% 2.8–20.9 chemical treatment, contrasting with 49.2–60.3% 37.1–108.5 control. Percent efficacies (±SD) computed weekly densities over period averaged 84.0 ± 1.6 85.3 1.8 versus 78.0 4.0 79.9 3.2 high‐rate low‐rate TBb, respectively, 84.5 7.8 treatment. Imidacloprid showed faster kill action but more variable efficacy than throughout trials. CONCLUSION Either or formulation competes reducing percent density. overall was significantly higher Bb. © 2024 Society Chemical Industry.

Language: Английский

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Recovery of insect-pathogenic fungi from solar UV damage: Molecular mechanisms and prospects

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 59 - 82

Published: Jan. 1, 2024

Language: Английский

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High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 251, P. 112849 - 112849

Published: Jan. 23, 2024

Language: Английский

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Divergent roles of Rad4 and Rad23 homologs in Metarhizium robertsii ’s resistance to solar ultraviolet damage

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(9)

Published: Sept. 1, 2023

The anti-ultraviolet (UV) role of a Rad4-Rad23-Rad33 complex in budding yeast relies on nucleotide excision repair (NER), which is mechanistically distinct from photorepair DNA lesions generated under solar UV irradiation but remains poorly known filamentous fungi. Here, two nucleus-specific Rad4 paralogs (Rad4A and Rad4B) nucleocytoplasmic shuttling Rad23 ortholog are functionally characterized by multiple analyses their null mutants Metarhizium robertsii, an entomopathogenic fungus lacking Rad33. Rad4A was proven to interact with contribute significantly more conidial UVB resistance (90%) than (65%). Despite no other biological function, exhibited very high activity photoreactivation UVB-impaired/inactivated conidia 5-h light exposure due its interaction Rad10, anti-UV protein clarified previously have acquired similar through photolyase M. robertsii. NER or revealed lower reactivation rates moderately impaired after 24-h dark incubation hardly observable at the end 12-h incubation, suggesting infeasibility field where nighttime too short. Aside remarkable contribution resistance, had pleiotropic effect radial growth, aerial conidiation, antioxidant response, cell wall integrity activity. However, Rad4B proved redundant function. unveils essentiality for robertsii's fitness homolog's depending NER. IMPORTANCE Resilience ultraviolet (UV)-impaired cells crucial application fungal insecticides based formulated conidia. Anti-UV roles Rad4, Rad23, Rad33 rely upon (NER) yeast. Among robertsii Rad33, contributes interacts rather Rad4B. acquires severely inactivated another photorepair-required photolyase. either seemingly extant unfeasible conditions. has asexual cycle vitro Therefore, strong depends photoreactivation, unveiling scenario NER-reliant role.

Language: Английский

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A Novel Deep Learning Model for Accurate Pest Detection and Edge Computing Deployment

Insects, Journal Year: 2023, Volume and Issue: 14(7), P. 660 - 660

Published: July 24, 2023

In this work, an attention-mechanism-enhanced method based on a single-stage object detection model was proposed and implemented for the problem of rice pest detection. A multi-scale feature fusion network first constructed to improve model’s predictive accuracy when dealing with pests different scales. Attention mechanisms were then introduced enable focus more areas in images, significantly enhancing performance. Additionally, small knowledge distillation designed edge computing scenarios, achieving high inference speed while maintaining accuracy. Experimental verification IDADP dataset shows that outperforms current state-of-the-art models terms precision, recall, accuracy, mAP, FPS. Specifically, mAP 87.5% FPS value 56 achieved, outperforming other comparative models. These results sufficiently demonstrate effectiveness superiority method.

Language: Английский

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Comparative efficacies of two fungal insecticides in field control of cereal aphids heavily infesting winter wheat

BioControl, Journal Year: 2024, Volume and Issue: 69(4), P. 437 - 448

Published: May 30, 2024

Language: Английский

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Photoreactivation Activities of Rad5, Rad16A and Rad16B Help Beauveria bassiana to Recover from Solar Ultraviolet Damage

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 420 - 420

Published: June 13, 2024

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair nucleotide excision of ultraviolet-induced DNA damage; however, their homologs have not yet been studied non-yeast fungi. fungus Beauveria bassiana, a deficiency Rad7 homolog, ortholog two Rad16 paralogs (Rad16A/B) instituted an ability to help insect-pathogenic recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were altered absence rad5, rad16A or rad16B, while severe defects growth conidiation caused by double deletion rad16B. Compared with wild-type complemented strains, mutants showed differentially reduced activities regarding resilience UVB-impaired conidia at 25 °C 12-h incubation regime visible light plus dark (L/D 3:9 h 5:7 for photoreactivation) full darkness (dark reactivation) mimicking natural nighttime. estimates median lethal dose LD50 L/D treatments revealed greater Rad16B than Rad16A additive either NER-dependent reactivation photorepair-dependent However, limited recovering low dose-impaired but unable impaired sublethal doses as did photoreactivation 5:7, unless night/dark time was doubled further prolonged. Therefore, anti-UV effects Rad5, B. bassiana depend primarily on are mechanistically distinct those yeast homologs.

Language: Английский

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Effect of pesticide application techniques on thrips control in the cowpea flowering stage

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Thrips are among the most damaging pests to cowpeas in Hainan, China. Conventional pesticide application methods often fail achieve satisfactory control due small size and concealed habitats of thrips. This study aimed enhance efficacy pesticides by improving their techniques. The optimal time day was identified, physicochemical properties four types spray adjuvants were tested evaluate impact on efficacy. A terrestrially targeted air-assisted sprayer for designed adjust wind direction target at thrips hiding spots. Field trials conducted Hainan assess modified times, adjuvant addition, spraying.

Language: Английский

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