Streamlining the Identification of the Orange Spiny Whitefly, Aleurocanthus spiniferus (Hemiptera: Aleyrodidae), with Real-Time PCR Probe Technology

Agriculture, Journal Year: 2025, Volume and Issue: 15(4), P. 414 - 414

Published: Feb. 16, 2025

Aleurocanthus spiniferus (Quaintance) (Hemiptera: Aleyrodidae) has rapidly spread, mainly in the central and eastern Mediterranean coastal area, infesting various new host plants alongside known ones. This invasive species poses a significant threat to agricultural ecosystems, necessitating urgent action monitor control outbreaks previously pest-free areas. While entomological morphological recognitions are crucial for initial detection, challenges often arise quickly identifying different developmental stages or genus-level distinctions, particularly surveys conducted by personnel with limited skills. Due these challenges, qPCR probe protocol was developed enhance diagnostic capacity of laboratories responsible territorial pests. biomolecular tool integrates surveys, enabling prompt reliable proof A. presence free areas, delimited territories, during phytosanitary import inspections. The protocol’s high analytical specificity, inclusivity, exclusivity ensure accurate identification spiniferus, while its low limit detection repeatability reproducibility reinforce utility as standardized method. By facilitating targeted efforts, this innovative approach strengthens resilience systems against widespread infestations.

Language: Английский

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A simple PCR-based quick detection of the economically important oriental fruit fly, Bactrocera dorsalis (Hendel) from India

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 9, 2024

The oriental fruit fly, Bactrocera dorsalis (Hendel), is a significant economic and quarantine pest due to its polyphagous nature. accurate identification of B. challenging at the egg, maggot, pupal stages, lack distinct morphological characters similarity other flies. Adult requires specialized taxonomist. Existing methods are laborious, time consuming, expensive. Rapid precise crucial for timely management. By analyzing variations in mitochondrial cytochrome oxidase-1 gene sequence (Insect barcoding gene), we developed species-specific primer (SSP), DorFP1/DorRP1, . optimal annealing temperature SSP was determined be 66°C, with no cross-amplification or primer-dimer formation observed. validated specimens from various locations northern eastern India tested cross-specificity six economically fly species India. specificity further confirmed by analysis critical threshold (Ct) value qPCR assay. Sensitivity showed could detect template DNA concentrations as low 1 pg/µl, though sensitivity decreased lower concentrations. Sequencing SSP-amplified product revealed over >99% existing sequences NCBI GenBank. reliably identifies across all developmental stages sexes. This assay expected significantly impact identification, phytosanitary measures, eradication programs

Language: Английский

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Identification of the Melon Fruit Fly Zeugodacus cucurbitae (Coquillett) (Diptera, Tephritidae) by Real-Time PCR with SYBR Green I Dye

Entomological Review, Journal Year: 2024, Volume and Issue: 104(5), P. 324 - 336

Published: Aug. 1, 2024

Language: Английский

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