Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 180 - 180

Published: Feb. 8, 2025

Chelonus formosanus is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive Spodoptera frugiperda, and it demonstrates strong pest control potential. Both egg larval stages primarily occur within host organism, total developmental time from to adult approximately 19.62 days. To investigate microbial communities at different stages, we performed 16S rDNA sequencing (V1–V9 region) using PacBio identified 404 bacterial species belonging 61 classes, 116 orders, 182 families, 308 genera across larval, pupal, female, male stages. Bacterial diversity richness varied with Enterobacter Enterococcus dominating in larvae pupae Pseudomonas emerging as dominant female adults. In contrast, adults predominantly resided Ralstonia Achromobacter. The predicted functions bacteria C. are marked by high abundance metabolic pathways. This study provides comprehensive understanding morphology contributes practical pests. Additionally, our findings preliminarily characterized community laying groundwork for its functional study.

Language: Английский

Effects of Feeding Sources and Different Temperature Changes on the Gut Microbiome Structure of Chrysomya megacephala (Diptera: Calliphoridae)

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 283 - 283

Published: March 8, 2025

Chrysomya megacephala (Diptera: Calliphoridae), commonly referred to as the oriental latrine fly, is a synanthropic blowfly species frequently associated with decomposing organic matter. This study sought investigate influence of various feeding substrates and temperature conditions, specifically constant temperatures 15, 25, 35 °C, variable averaging 23.31 on gut microbiome C. megacephala. The analysis was conducted using Illumina HiSeq platform for 16S rRNA gene sequencing in Changsha, China. Across all experimental yielded 1257 operational taxonomic units (OTUs), which were categorized into 26 phyla, 72 classes, 165 orders, 270 families, 516 genera, 794 species. showed significant differences between different sources conditions across lifespan. Low had potential reduce proportion abundance bacterial communities microbiome, while high increased them. Metabolism main predicted function diverse phenotypic characters microbiota presence phenotypes highlights its interest medicine offers promising applications industry agriculture.

Language: Английский