Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae) DOI Creative Commons
Guofeng Yang,

Xuetao Yu,

Yan Zhang

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 942 - 942

Published: Nov. 29, 2024

Epicauta gorhami is a hypermetamorphic insect that mainly forage soybeans during the adult stage. However, lack of appropriate references hinders our studying gene function in E. gorhami. In this study, referring to five computational tools (Ct value, geNorm, NormFinder, BestKeeper and RefFinder), stability 10 housekeeping genes (GAPDH, ACT, RPL4, RPL27, α-TUB, RPS18, EF1α, RPS28, RPL13 SOD) was assessed by qRT-PCR under three different conditions (adult ages, tissues/organs temperatures). The findings suggested SOD RPS18 were most ideal for examine transcripts among diverse ages at various temperatures; pair RPS28 reliable assess expressions tissues. Finally, relative expression levels EgUAP computed after normalization with across As expected, abundant foregut, trachea antenna scarce midgut, hindgut epidermis. These will lay solid foundation analyzing

Language: Английский

Evaluation and Validation of Reference Genes for Gene Expression Analysis Using qRT-PCR in the Sugarcane Stem Borer Chilo sacchariphagus (Lepidoptera: Pyralidae) DOI Creative Commons
Zhixiong Wang, Shang XianKun, Ji-Li Wei

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(8), P. 594 - 594

Published: Aug. 4, 2024

(Lepidoptera: Pyralidae) is an economically important sugarcane pest. Although numerous studies were conducted on the physiological responses in

Language: Английский

Citations

3
Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in Holotrichia parallela under Diverse Biological Conditions and Environmental Stresses DOI Creative Commons
Zhongjun Gong, Jing Zhang, Qi Chen

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 661 - 661

Published: Aug. 30, 2024

is among the world's most destructive pests. For accurate qPCR and gene expression studies, selection of stable appropriate reference genes crucial. However, a thorough evaluation potential for use in

Language: Английский

Citations

1
Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach Parasitoid Tetrastichus hagenowii (Ratzeburg) DOI Creative Commons
Renke Dong,

Fengming Cao,

Jincong Yu

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 668 - 668

Published: Sept. 3, 2024

Parasitoid wasps play a crucial role in the efficient control of pests, substantial menace to human health and well-being.

Language: Английский

Citations

1
Reference Gene Selection for RT-qPCR Normalization in Toxoplasma gondii Exposed to Broxaldine DOI Open Access
Yanhua Qiu, Yubin Bai, Weiwei Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11403 - 11403

Published: Oct. 23, 2024

Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is widely used to accurately assess target gene expression. Evaluating expression requires the selection of appropriate reference genes. To identify reliable genes for

Language: Английский

Citations

1
Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae) DOI Creative Commons
Guofeng Yang,

Xuetao Yu,

Yan Zhang

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(12), P. 942 - 942

Published: Nov. 29, 2024

Epicauta gorhami is a hypermetamorphic insect that mainly forage soybeans during the adult stage. However, lack of appropriate references hinders our studying gene function in E. gorhami. In this study, referring to five computational tools (Ct value, geNorm, NormFinder, BestKeeper and RefFinder), stability 10 housekeeping genes (GAPDH, ACT, RPL4, RPL27, α-TUB, RPS18, EF1α, RPS28, RPL13 SOD) was assessed by qRT-PCR under three different conditions (adult ages, tissues/organs temperatures). The findings suggested SOD RPS18 were most ideal for examine transcripts among diverse ages at various temperatures; pair RPS28 reliable assess expressions tissues. Finally, relative expression levels EgUAP computed after normalization with across As expected, abundant foregut, trachea antenna scarce midgut, hindgut epidermis. These will lay solid foundation analyzing

Language: Английский

Citations

0