Molecular Diagnostics for Monitoring Insecticide Resistance in Lepidopteran Pests DOI Creative Commons
Camila Ivo C. Vilarinho Fernandes Junqueira, Eliza F. M. B. Nascimento, Leonardo A. Vidal

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2553 - 2553

Published: Oct. 31, 2024

Chemical control methods to prevent crop damage have long been directly implicated in the selection of lepidoptera insect populations resistant insecticides. More recently, new products featuring different modes action (MoA), developed mitigate negative effects management on both producers and environment, are rapidly losing efficacy due emergence insects. Among these, certain resistances associated with molecular changes genomes pest insects that valuable for developing markers diagnostic tools, particularly point mutations. Molecular diagnosis represents an innovative solution insecticide resistance (IRM) practices, allowing effective monitoring resistance. This approach facilitates decision making by enabling timely alternation between (MoAs). In this context, review focuses major lepidopteran pests affect globally significant crops, discussing impacts It gathers literature methods; provides a comparative overview advantages techniques terms efficiency, cost, precision, sensitivity, applicability; highlights several novel tools. Additionally, explores coffee leaf miner, Leucoptera coffeella, as applied model illustrate potential approaches more sustainable strategies.

Language: Английский

CRISPR/Cas: An emerging molecular technology for biological control of fall armyworm DOI Creative Commons
Ehab El‐Awaad, Hans Merzendorfer

New plant protection., Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Abstract The fall armyworm, Spodoptera frugiperda , is a highly invasive pest that poses severe threats to food crop production worldwide. Several methods have been applied control armyworm infestations, including egg parasitoids, chemical insecticides, and transgenic crops expressing Bacillus thuringiensis (Bt) toxins. However, the currently available are either poorly effective or facing challenges such as resistance development environmental concerns. rapidly evolving molecular tools of genetic engineering, particularly clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas), increasingly utilized in recent years attempts develop novel management strategies genetically resistant crops. CRISPR/Cas‐based genome editing offers precise efficient tool has used successfully several functional genomic studies over last few years. In this review, we summarize progress using CRISPR/Cas for enabling targeted modification key genes essential survival propagation pest. potential system insect‐resistant approaches can be integrated into current discussed.

Language: Английский

Citations

0
Acaricide Resistance Monitoring and Structural Insights for Precision Tetranychus urticae Management DOI Creative Commons

Said Kewedar,

Qing Chen, Timothy W. Moural

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 440 - 440

Published: April 23, 2025

The two-spotted spider mite (Tetranychus urticae) is a highly destructive and economically significant pest in agricultural, horticultural, ornamental agroecosystems worldwide, including hop (Humulus lupulus) mint (Mentha spp.) fields the Pacific Northwest (PNW) region of United States. Repeated acaricide applications rotations have led to widespread resistance, resulting control failures. In this study, we investigated mechanisms resistance four different acaricides (bifenthrin, bifenazate, etoxazole, abamectin) across 23 field-collected TSSM populations by integrating diagnostic bioassays, genetic screening for resistance-associated mutations, structural modeling, molecular docking. Several kdr mutations mutation combinations were detected TuVGSC all tested populations. G132A Tucytb was identified 68.75% 40% populations, while I1017F TuCHS 1 found 94% 100% Structural analysis revealed key interactions between target proteins both wild-type mutant variants, providing novel insights into functional impacts these mutations. Our findings enhance understanding adaptation among crops, supporting development more effective management strategies mitigate economic losses hops, mint, other crop production.

Language: Английский

Citations

0
Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

0
The impact of three thioxothiazolidin compounds on trehalase activity and development of Spodoptera frugiperda larvae DOI Creative Commons
Yan Wu,

Shangrong Hu,

Qixuan Mao

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18233 - e18233

Published: Oct. 9, 2024

Trehalases (TREs), serving as crucial enzymes regulating trehalose and chitin metabolism in insects, represent prime targets for pest control strategies. We investigated the impact of three thioxothiazolidin compounds (1G, 2G, 11G) on TRE activity summarized their effects growth development Spodoptera frugiperda (Lepidoptera, Noctuidae). The experimental larvae S. were injected with 11G), while group received an equivalent volume 2% DMSO a control. All had strong effect inhibiting activity, significantly prolonging pre-pupal stage. However, compared 11G-treated group, survival rate treated 1G 2G was reduced by 31.11% 27.78% respectively, occurrence phenotypic abnormalities related to higher. These results manifest that only inhibitors, modulate pathways larvae, ultimately resulting failure molting reduction rates. Consequently, compounds, hold potential environmentally friendly insecticides.

Language: Английский

Citations

1
Molecular Diagnostics for Monitoring Insecticide Resistance in Lepidopteran Pests DOI Creative Commons
Camila Ivo C. Vilarinho Fernandes Junqueira, Eliza F. M. B. Nascimento, Leonardo A. Vidal

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2553 - 2553

Published: Oct. 31, 2024

Chemical control methods to prevent crop damage have long been directly implicated in the selection of lepidoptera insect populations resistant insecticides. More recently, new products featuring different modes action (MoA), developed mitigate negative effects management on both producers and environment, are rapidly losing efficacy due emergence insects. Among these, certain resistances associated with molecular changes genomes pest insects that valuable for developing markers diagnostic tools, particularly point mutations. Molecular diagnosis represents an innovative solution insecticide resistance (IRM) practices, allowing effective monitoring resistance. This approach facilitates decision making by enabling timely alternation between (MoAs). In this context, review focuses major lepidopteran pests affect globally significant crops, discussing impacts It gathers literature methods; provides a comparative overview advantages techniques terms efficiency, cost, precision, sensitivity, applicability; highlights several novel tools. Additionally, explores coffee leaf miner, Leucoptera coffeella, as applied model illustrate potential approaches more sustainable strategies.

Language: Английский

Citations

0