Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome DOI Creative Commons
Patrizia Robino, Livio Galosi, Alessandro Bellato

et al.

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: Aug. 7, 2024

Abstract Background In this study, a probiotic mixture (Honeybeeotic) consisting of seven bacterial strains isolated from unique population honeybees ( Apis mellifera ligustica ) was used. That honeybee located in the Roti Abbey locality Marche Region Italy, an area human activities, and genetic contamination other populations. The aim to investigate effects on innate immunity intestinal microbiome healthy common two hives same apiary. Hive A received diet 50% glucose syrup, while hive B syrup supplemented with probiotics, both administered daily for 1 month. To determine whether altered immune response, phenoloxidase activity hemolymph cellular subtype count were investigated. Additionally, metagenomic approaches used analyze gut microbiota composition function, considering critical role plays modulating host physiology. Results results revealed differences hemocyte populations between hives, as exhibited higher counts oenocytoids granulocytes. These findings indicated that dietary supplementation safe well-tolerated. Furthermore, significantly decreased (1.75 ± 0.19 U/mg) compared (3.62 0.44 U/mg, p < 0.005), suggesting improved state well-being honeybees, they did not require activation defense mechanisms. Regarding composition, modulated control, retaining core components causing positive negative variations. Notably, several genes, particularly KEGG genes involved amino acid metabolism, carbohydrate branched-chain (BCAA) transport, more abundant probiotic-fed group, effective nutritional supplement host. Conclusions This study advocated feeding induces beneficial immunological promoted balanced enhanced metabolic activities related digestion. use highly selected probiotics shown contribute overall improving their response health.

Language: Английский

Diptera as predators in biological control: applications and future perspectives DOI Creative Commons
Giovanni Burgio, Maria Luisa Dindo, Thomas Pape

et al.

BioControl, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Language: Английский

Citations

2
Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome DOI Creative Commons
Patrizia Robino, Livio Galosi, Alessandro Bellato

et al.

Biological Research, Journal Year: 2024, Volume and Issue: 57(1)

Published: Aug. 7, 2024

Abstract Background In this study, a probiotic mixture (Honeybeeotic) consisting of seven bacterial strains isolated from unique population honeybees ( Apis mellifera ligustica ) was used. That honeybee located in the Roti Abbey locality Marche Region Italy, an area human activities, and genetic contamination other populations. The aim to investigate effects on innate immunity intestinal microbiome healthy common two hives same apiary. Hive A received diet 50% glucose syrup, while hive B syrup supplemented with probiotics, both administered daily for 1 month. To determine whether altered immune response, phenoloxidase activity hemolymph cellular subtype count were investigated. Additionally, metagenomic approaches used analyze gut microbiota composition function, considering critical role plays modulating host physiology. Results results revealed differences hemocyte populations between hives, as exhibited higher counts oenocytoids granulocytes. These findings indicated that dietary supplementation safe well-tolerated. Furthermore, significantly decreased (1.75 ± 0.19 U/mg) compared (3.62 0.44 U/mg, p < 0.005), suggesting improved state well-being honeybees, they did not require activation defense mechanisms. Regarding composition, modulated control, retaining core components causing positive negative variations. Notably, several genes, particularly KEGG genes involved amino acid metabolism, carbohydrate branched-chain (BCAA) transport, more abundant probiotic-fed group, effective nutritional supplement host. Conclusions This study advocated feeding induces beneficial immunological promoted balanced enhanced metabolic activities related digestion. use highly selected probiotics shown contribute overall improving their response health.

Language: Английский

Citations

1