Glutathione S‐Transferase Contributes to the Resistance of Megalurothrips usitatus Against Lambda‐Cyhalothrin by Strengthening Its Antioxidant Defense Mechanisms DOI

Wenlan Dong,

Chaozheng Wang, Xia Li

et al.

Archives of Insect Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 117(4)

Published: Dec. 1, 2024

ABSTRACT The damage caused by Megalurothrips usitatus , a common pest, has significantly affected the Chinese vegetable industry. inappropriate application of chemical pesticides M. to become highly resistant conventional insecticides. Glutathione S‐transferase (GST), known for its multifunctional properties, contributes detoxification and antioxidation. It enhances insects' adaptability facilitating elimination lipid peroxidation products resulting from pyrethroid This research employed RT‐qPCR identify GST genes that exhibited significant expression in response lambda‐cyhalothrin stress. also quantified changes antioxidant apoptosis markers within under exposure. functional significance was validated assessing alterations defense system resistance following inhibition activity. study's outcomes indicated MuGSTs1 markedly upregulated stress ( p < 0.0001). activity effectively suppressed specific inhibitor, diethyl maleate, achieving an rate 64.05%. Following GST, overall capacity reduced 3.1‐fold compared with control, 7.91‐fold increase sensitivity lambda‐cyhalothrin. These findings confirm pivotal role oxidative their contribution development through enhanced defenses. offers valuable perspectives on adaptive reactions insects stressors, management formulation effective pest control strategies.

Language: Английский

Monitoring invertebrate pests on cowpea crops across China using eDNA metabarcoding DOI Open Access
Qi Chen,

Jun‐Xian Lv,

Wen Xie

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract BACKGROUND Monitoring arthropod pests and their natural enemies provides essential information for pest control decisions in agricultural production. Traditional monitoring methods, such as trapping visual surveys, have practical limitations because of time‐consuming preprocessing steps the need knowledgeable taxonomists. We tested environmental DNA (eDNA) metabarcoding approach cowpea ( Vigna unguiculata ) across seven climatic sub‐regions China, comparing it with traditional sample collection morphological identification methods. RESULTS showed that eDNA reveals a higher species richness arthropods than method. The method detected 163 species, which 72, 33 57 were pests, other insects, respectively, whereas only 70 species. There significant differences alpha‐ beta‐diversity on different sub‐regions. also found longitude, altitude geographical distance are associated diversity, but latitude, mean air temperature range not. CONCLUSION This study represents first use to investigate communities, providing novel monitoring. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Transgenic cowpea conferring insect resistance and glyphosate tolerance DOI

Haiyan Lin,

H. Zhong, Hongyu Gao

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract BACKGROUND Cowpea is a favorite vegetable around the world. In China, tender pods of cowpea are primary edible part. However, vulnerable to variety pests throughout its entire growth cycle. Lepidopteran insects, including legume pod borer (LPB, Maruca vitrata ), beet armyworm (BAW, Spodoptera exigua and tobacco cutworm (TCW, litura major production. Conventional pest management strategies predominantly rely on chemical pesticides, which not only increase production costs but also raise critical concerns regarding food safety environmental sustainability. Genetically modified (GM) technology offers promising solution by introducing insect resistance trait into plants. Unlike transgenic corn soybean, conferring has been fully explored utilized. RESULTS this study, Cry1Ab‐Vip3A fusion gene, C1V3 , was successfully introduced genome. Transgenic lines with broad‐spectrum lepidopteran were obtained an improved transformation method using glyphosate as selection. A line, CAP5, selected from 37 events, characterized single T‐DNA copy event insertion site at Chr5 Comprehensive evaluation for herbicide tolerance indicated that CAP5 highly resistant BAW, LPB TCW exhibited strong glyphosate, underscoring application potential insect‐resistant herbicide‐tolerant event. CONCLUSION line expressing protein CP4‐EPSPS LPB, strongly tolerant glyphosate. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Field-Based Evaluation of Insecticide Effectiveness on Megalurothrips usitatus in Guangdong, China: Implications for Pest Control Strategies DOI Creative Commons
Zhengke Peng,

M. Li,

Congcong Guo

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 459 - 459

Published: April 27, 2025

In southern China, cowpea production is severely threatened by Megalurothrips usitatus due to its fast-evolving resistance insecticides. The toxicity monitoring of commonly used insecticides against field populations provides key information for the management pests. this study, were collected from three locations (QY, YF, MM) in Guangdong, and sensitivity these was determined using a thrips bioassay system (TIBS) method. results indicated there variances between populations. Among 10 insecticides, spinetoram spinosad both showed high all addition, broflanilide QY, emamectin benzoate cyantraniliprole MM suggested as alternate alleviate selective pressure on experiments, corrected control efficacy M. over 75% at 7 dpa, which proved be ideal application. These field-based provide guidance chemical can valuable proposing appropriate strategies management.

Language: Английский

Citations

0
Determination of spinetoram residues in rice field environment by HPLC combined with QuEChERS method, residue elimination and final residues DOI
Xiaoxue Zhang,

Hailong Chang,

Lin Wei

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 134, P. 106529 - 106529

Published: July 15, 2024

Language: Английский

Citations

2
Glutathione S‐Transferase Contributes to the Resistance of Megalurothrips usitatus Against Lambda‐Cyhalothrin by Strengthening Its Antioxidant Defense Mechanisms DOI

Wenlan Dong,

Chaozheng Wang, Xia Li

et al.

Archives of Insect Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 117(4)

Published: Dec. 1, 2024

ABSTRACT The damage caused by Megalurothrips usitatus , a common pest, has significantly affected the Chinese vegetable industry. inappropriate application of chemical pesticides M. to become highly resistant conventional insecticides. Glutathione S‐transferase (GST), known for its multifunctional properties, contributes detoxification and antioxidation. It enhances insects' adaptability facilitating elimination lipid peroxidation products resulting from pyrethroid This research employed RT‐qPCR identify GST genes that exhibited significant expression in response lambda‐cyhalothrin stress. also quantified changes antioxidant apoptosis markers within under exposure. functional significance was validated assessing alterations defense system resistance following inhibition activity. study's outcomes indicated MuGSTs1 markedly upregulated stress ( p < 0.0001). activity effectively suppressed specific inhibitor, diethyl maleate, achieving an rate 64.05%. Following GST, overall capacity reduced 3.1‐fold compared with control, 7.91‐fold increase sensitivity lambda‐cyhalothrin. These findings confirm pivotal role oxidative their contribution development through enhanced defenses. offers valuable perspectives on adaptive reactions insects stressors, management formulation effective pest control strategies.

Language: Английский

Citations

2