Effect of honeybee queen size and HSP90 and HSC70 gene expression on thermal stress resistance DOI Creative Commons

Alireza Derafsh,

Abdolreza Salehi, Esmaeil Amiri

et al.

Frontiers in Bee Science, Journal Year: 2025, Volume and Issue: 3

Published: March 13, 2025

Introduction In beekeeping, queen cell size is a critical factor influencing the growth and development of bees. It was hypothesized that larger cells would produce queens with greater weight, enhanced resilience to heat stress, higher expression shock proteins ( HSP90 HSC70 ), leading improved survival under environmental stress. Materials methods This study, conducted in Shiraz Province, Iran, 2023, aimed test this hypothesis. A total 270 were divided into three groups based on size: large (10–9.5 mm), medium (9–8.5 small (7.5–7 mm). The reared using different methods: 1) simultaneous starter_finisher colonies, 2) separate 3) rearing presence queen. Since there types, resulted 90 for each method. Each group consisted subsets 30 cells: large, small, medium. From cells, 176 survived 94 died. Results discussion We stepwise procedure logistic model, results indicated which included type, method, birth showed best predictive performance. evidenced by lowest Akaike information criterion value. Then, from method 2, we placed 12 type two six subjected stress levels: low temperature 4°C high above 40°C. 36 frozen samples replicates treatment combination used molecular testing. Gene analysis real-time PCR evaluate gene expression. produced had significantly . These demonstrated superior rates high-temperature conditions compared smaller cells. Conclusions findings support hypothesis optimizing can enhance performance colony resilience. Our suggest promote development, resilience, rates, ultimately improving health productivity.

Language: Английский

Effect of honeybee queen size and HSP90 and HSC70 gene expression on thermal stress resistance DOI Creative Commons

Alireza Derafsh,

Abdolreza Salehi, Esmaeil Amiri

et al.

Frontiers in Bee Science, Journal Year: 2025, Volume and Issue: 3

Published: March 13, 2025

Introduction In beekeeping, queen cell size is a critical factor influencing the growth and development of bees. It was hypothesized that larger cells would produce queens with greater weight, enhanced resilience to heat stress, higher expression shock proteins ( HSP90 HSC70 ), leading improved survival under environmental stress. Materials methods This study, conducted in Shiraz Province, Iran, 2023, aimed test this hypothesis. A total 270 were divided into three groups based on size: large (10–9.5 mm), medium (9–8.5 small (7.5–7 mm). The reared using different methods: 1) simultaneous starter_finisher colonies, 2) separate 3) rearing presence queen. Since there types, resulted 90 for each method. Each group consisted subsets 30 cells: large, small, medium. From cells, 176 survived 94 died. Results discussion We stepwise procedure logistic model, results indicated which included type, method, birth showed best predictive performance. evidenced by lowest Akaike information criterion value. Then, from method 2, we placed 12 type two six subjected stress levels: low temperature 4°C high above 40°C. 36 frozen samples replicates treatment combination used molecular testing. Gene analysis real-time PCR evaluate gene expression. produced had significantly . These demonstrated superior rates high-temperature conditions compared smaller cells. Conclusions findings support hypothesis optimizing can enhance performance colony resilience. Our suggest promote development, resilience, rates, ultimately improving health productivity.

Language: Английский

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