Biocompatibility and photoinactivation evaluation of zinc hydroxide chloride nanosheets against Streptococcus mutans

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 4, 2025

Considering the efficacy of antimicrobial photodynamic therapy (PDT) in inactivating bacteria, this study reports that zinc hydroxide chloride nanosheets (ZHC-NSs) are useful for purpose. The characterization ZHC-NSs was performed using microscopic and spectroscopic techniques. irritation test, acute toxicity genotoxicity test were evaluated their effects on human pulp fibroblast cells (HPFC) viability, intracellular reactive oxygen species (ROS) levels, antibacterial activity (1-8 mg ml-1) alone or light conditions investigated. structure showed a crystalline form sheets' thickness had an average size 129.6 ± 19.50 nm. did not severely damage internal organs genotoxic. cytotoxic effect HPFC concentration-dependent so at higher concentrations (4 8 killed half cells. When used combination with 980 nm diode laser, namely ZHC-NS©, ROS production increased led to enhanced against Streptococcus mutans planktonic biofilm form. A statistically significant difference found between without laser irradiation photoexcited ZHC-NSs. ZHC-NSs© potential ability produce could be effective complementary treatment S. mutans.

Language: Английский

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Effect of temperature on the structure, catalyst and magnetic properties of un-doped zinc oxide nanoparticles: experimental and DFT calculation

RSC Advances, Journal Year: 2024, Volume and Issue: 14(42), P. 31153 - 31164

Published: Jan. 1, 2024

Zinc oxide nanoparticles were synthesized using sol–gel and hydrothermal techniques characterized at different calcination temperatures (400, 500, 600 °C).

Language: Английский

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Development, Characterization, and Antimicrobial Evaluation of Hybrid Nanoparticles (HNPs) Based on Phospholipids, Cholesterol, Colistin, and Chitosan Against Multidrug-Resistant Gram-Negative Bacteria

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 182 - 182

Published: Feb. 1, 2025

Background: Colistin, a lipopeptide antibiotic usually used as last resort against multidrug-resistant bacterial strains, has also begun to address the challenge of antimicrobial resistance. Objective: this study evaluates whether hybrid nanoparticles (HNPs) composed Phospholipon® 90G, cholesterol, and colistin can enhance its effectiveness resistant clinical isolates Klebsiella pneumoniae, clinically significant Gram-negative bacterium. Methods: HNPs were developed using ethanol injection method coated with chitosan through layer-by-layer technique. HNP characterization included measurements particle size, polydispersity index (PDI), zeta potential, along thermal (DSC) spectrophotometric (FT-IR) analyses. Ultrafiltration ATR-FTIR employed assess colistin’s association release efficiencies. The biological evaluation followed CLSI guidelines. Results: uncoated (U-HNP) chitosan-coated (Ch-HNP) described monodisperse populations, respective PDI values ~0.124 ~0.150, Z-averages ~249 nm ~250 nm, potential +17 mV +20 mV. Colistin’s efficiencies approximately 79% 10%, respectively. Regarding activity, results showed that part is poorly effective microorganism. However, in most strain, activity increased slightly when was chitosan. Conclusions: high stability disaggregation, limiting and, therefore, affecting performance.

Language: Английский

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Zinc as a Modulator of Male Fertility: Interplay Between Lipid Metabolism, Oxidative Stress, and Sperm Function

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

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