Combination of scaffold with adipose-derived mesenchymal stem cell on knee joint cartilage defects: A literature review DOI Open Access
Aliefio Japamadisaw, Kukuh Dwiputra Hernugrahanto, Dwikora Novembri Utomo

et al.

Edelweiss Applied Science and Technology, Journal Year: 2024, Volume and Issue: 8(6), P. 9592 - 9604

Published: Dec. 31, 2024

Knee joint cartilage defects are a significant clinical challenge due to the limited regenerative capacity of articular cartilage, often leading degeneration and osteoarthritis (OA). Conventional treatment methods, such as surgical repair conservative management, frequently fail restore function fully. In recent years, scaffold-based tissue engineering using adipose-derived mesenchymal stem cells (ADMSCs) has emerged promising strategy for regeneration. ADMSCs, known their abundant availability, ease isolation, chondrogenic potential, provide viable cellular source repairing damaged cartilage. When combined with biocompatible scaffolds, ADMSCs can enhance regeneration by promoting cell proliferation, differentiation, extracellular matrix (ECM) formation at injury site. Various scaffold materials, including natural synthetic polymers, have been explored structural support necessary attachment formation. This literature review examines current advancements in application ADMSC-loaded scaffolds knee defects, focusing on biological properties, designs, outcomes preclinical studies. The also addresses challenges, degradation, mechanical migration, that must be optimized successful translation. Overall, this highlights potential ADMSC-based systems offer solution provides insights into future directions improving therapeutic strategies engineering.

Language: Английский

Porous polytrimethylenecarbonate scaffolds: Design considerations and porosity modulation techniques DOI Creative Commons
Klaudia Małgorzata Jurczak, Ruichen Zhang, Wouter L.J. Hinrichs

et al.

Materials & Design, Journal Year: 2025, Volume and Issue: unknown, P. 113588 - 113588

Published: Jan. 1, 2025

Language: Английский

Citations

3
Integrating advanced synthesis techniques and AI-driven approaches with nanofiber technology: A state-of-the-art approach to wound care management DOI Creative Commons
Nisha Shankhwar, Awadhesh Kumar Verma, Ashab Noumani

et al.

Next Nanotechnology, Journal Year: 2025, Volume and Issue: 8, P. 100147 - 100147

Published: Jan. 1, 2025

Language: Английский

Citations

1
From Bone To Blood Flow: Tissue Engineering In Orthopedics – A Narrative Review DOI Creative Commons
Bshara Sleem,

Rakan Nassereldine,

Victor Ghazi

et al.

Orthopedic Reviews, Journal Year: 2025, Volume and Issue: 17

Published: March 31, 2025

Musculoskeletal injuries and degenerative conditions necessitate advanced regenerative solutions. Tissue engineering has emerged as a pivotal field in orthopedic care, particularly vascularized bone cartilage regeneration. This narrative review examines the latest advancements vascular tissue engineering, including scaffold design, cell-based techniques, growth factor delivery. A comprehensive literature search was conducted using PubMed, ScienceDirect, Google Scholar, focusing on innovations challenges field. Vascularized grafts (VBGs) outperform non-vascularized counterparts promoting healing integration. Advances materials, such smart scaffolds hybrid biomaterials, enhance osteogenesis angiogenesis. Cellular therapies, utilizing mesenchymal stem cells induced pluripotent cells, synergistically improve vascularization Growth factors like VEGF morphogenic protein (BMP-2), integrated with innovative delivery systems, enable sustained angiogenic stimulation While significant strides have been made, persist achieving full integration replicating native architecture. Innovations technology surgery techniques hold promise for transforming improving patient outcomes.

Language: Английский

Citations

1
Fabrication of PVTF Electroactive Coatings on PEEK Implant to Provide Surface Potential for Enhancing Osteogenesis DOI Open Access
Albert F. Yee, Xin Xin,

Haoqing Liu

et al.

Coatings, Journal Year: 2025, Volume and Issue: 15(3), P. 261 - 261

Published: Feb. 22, 2025

Polyetheretherketone (PEEK) has gained significant attention in biomedical applications due to its excellent mechanical properties and biocompatibility. In this work, the fabrication of electroactive poly(vinylidenefluoride-co-trifluoroethylene) (PVTF) coatings on PEEK surfaces enhance osteogenesis is explored. substrates were prepared with different surface treatments optimize adhesion, followed by PVTF coating through drop-casting polarization. Morphological, chemical, thermal characterizations revealed uniform β-phase crystallization layer, achieving a peak piezoelectric coefficient (d33) 16 pC/N under 4 kV polarization voltage. Cell culture experiments demonstrated improved biocompatibility, polarized showing enhanced bone marrow mesenchymal stem cell (BMSC) proliferation, osteogenic differentiation. ALP activity, key marker osteogenesis, was significantly higher samples. Furthermore, modified exhibited strong adhesion between PEEK, as well sustained potential physiological conditions. These test results indicate that PEEK/PVTF composite, shows great an material for implants.

Language: Английский

Citations

0
Regeneration of Bone Tissue Using Nanofibers Made from Electrospun Polycaprolactone (PCL) and a Hydrogel Composed of Alginate (Alg/PCL) DOI
Fatemeh Ashrafi, Asrin Emami, Mona Navaei‐Nigjeh

et al.

Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Language: Английский

Citations

0
Cu-MOF-Decorated 3D-Printed Scaffolds for Infection Control and Bone Regeneration DOI Creative Commons
Ting Zhu, Qi Ni,

Wenjie Wang

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(3), P. 83 - 83

Published: March 1, 2025

Infection control and bone regeneration remain critical challenges in defect treatment. We developed a 3D-printed scaffold incorporating copper-based metal–organic framework-74 (Cu-MOF-74) within polycaprolactone/hydroxyapatite composite. The synthesized Cu-MOF-74 exhibited well-defined crystalline structure rod-like morphology, as confirmed by TEM, EDS, FTIR, XRD analyses. scaffolds hierarchical pores (100–200 μm) demonstrated tunable hydrophilicity, evidenced the water contact angles decreasing from 103.3 ± 2.02° (0% Cu-MOF-74) to 63.60 1.93° (1% Cu-MOF-74). A biphasic Cu2+ release profile was observed scaffolds, reaching cumulative concentrations of 98.97 3.10 ppm day 28. Antimicrobial assays showed concentration-dependent efficacy, with 1% achieving 90.07 1.94% 80.03 2.17% inhibition against Staphylococcus aureus Escherichia coli, respectively. Biocompatibility assessments using marrow-derived mesenchymal stem cells revealed enhanced cell proliferation at ≤ 0.2%, while ≥ 0.5% induced cytotoxicity. Osteogenic differentiation studies highlighted elevated alkaline phosphatase activity mineralization 0.05–0.2% particularly 0.05% which highest calcium deposition upregulation sialoprotein osteopontin expression. These findings demonstrate dual functional efficacy Cu-MOF-74/PCL/HAp promoting both infection regeneration. optimized (0.05–0.2%) effectively balance antimicrobial osteogenic properties, presenting promising strategy for repair clinical applications.

Language: Английский

Citations

0
Biopolymeric Scaffolds with Melatonin for Tissue Engineering—A Review DOI Open Access

Beata Kaczmarek,

Sylwia Grabska-Zielińska

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2520 - 2520

Published: March 11, 2025

Melatonin, a natural hormone with antioxidant, anti-inflammatory, and regenerative properties, has gained increasing attention in tissue engineering for its ability to enhance the therapeutic potential of biopolymeric scaffolds. These scaffolds, designed mimic extracellular matrix, provide structural support bioactive environment regeneration. By integrating melatonin, researchers aim create multifunctional scaffolds that promote cell proliferation, modulate inflammatory responses, improve wound healing outcomes. Challenges utilizing melatonin include maintaining stability under light, heat, oxygen exposure, optimizing release profile sustained effects. Innovative fabrication methods, such as electrospinning, 3D printing, lyophilization, have enabled precise control over scaffold architecture delivery. techniques ensure enhanced interactions target tissues tailored regeneration processes. Combining growth factors, cytokines, antimicrobial agents offers applications, from chronic management bone nerve Continued research this field promises transformative solutions medicine, expanding clinical applicability melatonin-enriched This review highlights current progress, challenges, opportunities associated harnessing melatonin’s within frameworks.

Language: Английский

Citations

0
Exploring a Nitric Oxide-Releasing Celecoxib Derivative as a Potential Modulator of Bone Healing: Insights from Ex Vivo and In Vivo Imaging Experiments DOI Open Access
Christin Neuber,

Luisa Niedenzu,

Sabine Schulze

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2582 - 2582

Published: March 13, 2025

The inducible enzyme cyclooxygenase-2 (COX-2) and the subsequent synthesis of eicosanoids initiated by this are important molecular players in bone healing. In pilot study, suitability a novel selective COX-2 inhibitor bearing nitric oxide (NO)-releasing moiety was investigated as modulator healing critical-size defect rats. A 5 mm femoral randomly filled with no material (negative control, NC), mixture collagen autologous fragments (positive PC), or polycaprolactone-co-lactide (PCL)-scaffolds coated two types artificial extracellular matrix (aECM; collagen/chondroitin sulfate (Col/CS) collagen/polysulfated hyaluronic acid (Col/sHA3)). Bone monitored dual-tracer ([18F]FDG/[18F]fluoride) approach using PET/CT imaging vivo. addition, ex vivo µCT well histological immunohistochemical studies were performed 16 weeks post-surgery. significant higher uptake [18F]FDG, surrogate marker for inflammatory infiltrate, but not [18F]fluoride, representing mineralization, observed implanted PCL-scaffolds either Col/CS Col/sHA3. Molecular targeting NO-coxib had effect on tracer any groups. Histological staining showed evidence positive negative influence treatment

Language: Английский

Citations

0
Valorization of Sugarcane Bagasse into Cellulose Nanofiber Containing Phosphate Groups: A New Scaffold for in Vitro Calcium Phosphate Mineralization DOI
Naglaa Salem El‐Sayed, Emad Tolba, Ahmed Salama

et al.

Chemistry Africa, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Language: Английский

Citations

0
Advances in bioink-based 3D printed scaffolds: optimizing biocompatibility and mechanical properties for bone regeneration DOI
Pawan Kumar, Jitender Sharma, Ravinder Kumar

et al.

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The development of bioink-based 3D-printed scaffolds has revolutionized bone tissue engineering (BTE) by enabling patient-specific and biomimetic constructs for regeneration.

Language: Английский

Citations

0