Comparative Evaluation of Commercial, Freely Available, and Open-Source Tools for Single-Cell Analysis Within Freehand-Defined Histological Brightfield Image Regions of Interest

Technologies, Journal Year: 2025, Volume and Issue: 13(3), P. 110 - 110

Published: March 7, 2025

In the field of histological analysis, one typical issues is analysis single cells contained in regions interest (i.e., ROIs). Today, several commercial, freely available, and open-source software options are accessible for this task. However, literature lacks recent extensive reviews that summarise functionalities opportunities currently available provide guidance on selecting most suitable option analysing specific cases, instance, irregular freehand-defined ROIs brightfield images. work, we reviewed compared 14 tools tailored single-cell within a 2D image ROI. Precisely, six CellProfiler, Cytomine, Digital Slide Archive, Icy, ImageJ/Fiji, QuPath), four Aperio ImageScope, NIS Elements Viewer, Sedeen, SlideViewer), commercial Amira, Arivis, HALO, Imaris) were considered. We focused three key aspects: (a) capacity to handle large file formats such as SVS, DICOM, TIFF, ensuring compatibility with diverse datasets; (b) flexibility defining ROIs, whether through automated extraction or manual delineation, encompassing square, circular, polygonal, freehand shapes accommodate varied research needs; (c) capability classify selected images, ranging from fully semi-automated approaches, requiring different levels user involvement. Thanks deeper understanding strengths limitations platforms emerges, facilitating informed decision making researchers looking tool analyse

Language: Английский

Innovative approaches to blood-brain barrier modelling: The chip revolution

Next Nanotechnology, Journal Year: 2025, Volume and Issue: 7, P. 100171 - 100171

Published: Jan. 1, 2025

Language: Английский