Folate-Mediated One-Carbon Metabolism in the Crustacean Copepod Calanus finmarchicus: Identification of Transcripts and Relative Expression across Development DOI Creative Commons
Daniela Ascione, Ylenia Carotenuto, Chiara Lauritano

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 786 - 786

Published: May 8, 2024

Folate, also known as vitamin B9, plays a crucial role in the one-carbon (1C) metabolism, conserved pathway from microbes to humans. The 1C consisting of folate and methionine cycles, is essential many biological processes such nucleotide protein biosynthesis, cell proliferation, embryonic development. Despite its functional role, little about metabolism crustaceans. As part an ongoing effort characterize important pathways Calanus finmarchicus, biomass-dominant zooplankton much North Atlantic Ocean, we identified transcripts encoding enzymes. Using silico workflow transcriptome mining, reciprocal blasts, structural analyses deduced proteins, entire set enzymes both cycles. majority encoded for full-length proteins clustered with homologs other species. Stage-specific expression was reported, several showing high naupliar stage (e.g., 10-FTHFD, SHMT2) while some methyltransferases BHMT, SHMT, DNMT) were more expressed adults. Overall, this study provides genes which can be used potential biomarkers development reproduction tested zooplankters assess ocean health status monitoring.

Language: Английский

Ecology of Marine Zooplankton DOI Creative Commons
Marco Uttieri, Ylenia Carotenuto, Iole Di Capua

et al.

Journal of Marine Science and Engineering, Journal Year: 2023, Volume and Issue: 11(10), P. 1875 - 1875

Published: Sept. 27, 2023

Marine ecosystems, from coastal areas to open waters, teem with a multitude of heterotrophic and mixotrophic organisms collectively forming the zooplankton, animal component plankton [...]

Language: Английский

Citations

23
The Chemical Defensome: A Survey of Environmental Sensing and Response Genes in Copepods DOI Open Access
Vittoria Roncalli, Daniela Ascione, Chiara Lauritano

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1546 - 1546

Published: Feb. 12, 2025

Highly conserved among eukaryotes, the chemical defensome protects organisms against stressors and helps to reestablish altered homeostatic state. The includes genes such as transporters (e.g., adenosine triphosphate ATP-binding cassette), phase I II detoxification enzymes, antioxidant enzymes. During their life cycle, planktonic copepods, most abundant ubiquitous metazoans on Earth, are exposed many environmental that impair survival fitness. Here, using high-quality publicly available transcriptomic data, were searched in copepods belonging different orders living environments Antarctic, Subarctic, Mediterranean). Gene expression responses investigated four calanoids stresses identify a common species-specific system. Our results confirm is highly but also report differences relative contribution of species habitats, suggesting fitness adaptation pressures. provided here can be used biomarkers defense tested other assess "health" marine organisms, which useful for understanding adaptations they changes make predictions at population community levels.

Language: Английский

Citations

0
Transcriptomic analysis reveals responses to a polluted sediment in the Mediterranean copepod Acartia clausi DOI
Flavio Rotolo, Vittoria Roncalli, Matthew Cieslak

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 335, P. 122284 - 122284

Published: Aug. 3, 2023

Language: Английский

Citations

8
Folate-Mediated One-Carbon Metabolism in the Crustacean Copepod Calanus finmarchicus: Identification of Transcripts and Relative Expression across Development DOI Creative Commons
Daniela Ascione, Ylenia Carotenuto, Chiara Lauritano

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(5), P. 786 - 786

Published: May 8, 2024

Folate, also known as vitamin B9, plays a crucial role in the one-carbon (1C) metabolism, conserved pathway from microbes to humans. The 1C consisting of folate and methionine cycles, is essential many biological processes such nucleotide protein biosynthesis, cell proliferation, embryonic development. Despite its functional role, little about metabolism crustaceans. As part an ongoing effort characterize important pathways Calanus finmarchicus, biomass-dominant zooplankton much North Atlantic Ocean, we identified transcripts encoding enzymes. Using silico workflow transcriptome mining, reciprocal blasts, structural analyses deduced proteins, entire set enzymes both cycles. majority encoded for full-length proteins clustered with homologs other species. Stage-specific expression was reported, several showing high naupliar stage (e.g., 10-FTHFD, SHMT2) while some methyltransferases BHMT, SHMT, DNMT) were more expressed adults. Overall, this study provides genes which can be used potential biomarkers development reproduction tested zooplankters assess ocean health status monitoring.

Language: Английский

Citations

1