A mathematical phase field model predicts superparamagnetic nanoparticle accelerated fusion of HeLa spheroids for field guided biofabrication DOI Creative Commons
Cristian F. Rodríguez, Valentina Quezada, Paula Guzmán-Sastoque

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: June 5, 2025

In vitro tissue models are crucial for regenerative medicine, drug discovery, and the reduction of animal testing. 3D bioprinting, particularly when utilizing magnetic manipulation cell spheroids, provides precise control over architecture. However, existing mathematical lack precision to capture interplay between biological dynamics forces during spheroid fusion. This study developed validated a novel model that simulates magnetically assisted fusion, taking into account migration, adhesion, effects external fields. The integrates principles mechanics, fluid dynamics, magnetostatics, implemented in COMSOL Multiphysics. Experimental validation used HeLa spheroids bioprinted with superparamagnetic iron oxide nanoparticles (SPIONs). Spheroid fusion was monitored without an field using confocal microscopy. Rigorous statistical analysis (MAE, RMSE, MAPE, R², Chi-Square, Bland-Altman, variance-weighted metrics) evaluate performance. accurately predicted accelerated under manipulation, reducing time from approximately 7 days (without field) 2 days. High R² values (> 0.99 two-spheroid > 0.97 multi-spheroid systems) narrow confidence intervals demonstrated strong agreement simulation experiment. Increased system complexity introduced slightly higher error variability, but maintained robust predictive capabilities. disassembly observed four-spheroid case, highlighting complex cellular reorganization. validated, high-precision represents significant advancement engineering, providing powerful tool optimizing bioprinting protocols, designing constructs, advancing development. breakthrough has implications medicine discovery while also importance addressing nanoparticle safety concerns.

Language: Английский

Magnetoliposomes for nanomedicine: synthesis, characterization, and applications in drug, gene, and peptide delivery DOI
Cristian F. Rodríguez, Paula Guzmán-Sastoque,

Alan Santacruz-Belalcazar

et al.

Expert Opinion on Drug Delivery, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Magnetoliposomes represent a transformative advancement in nanomedicine by integrating magnetic nanoparticles with liposomal structures, creating multifunctional delivery platforms that overcome key limitations of conventional drug carriers. These hybrid systems enable precision targeting through external fields, controlled release via hyperthermia, and real-time theranostic capabilities, offering unprecedented spatiotemporal control over therapeutic administration. This manuscript focused primarily on studies from 2023-2025 however, few select older references were included to provide background context.This review examines the fundamental design principles Magnetoliposomes, including bilayer composition, nanoparticle integration strategies, physicochemical properties governing their biological performance. We comprehensively assess synthesis methodologies - traditional thin-film hydration advanced microfluidic approaches highlighting impact colloidal stability, encapsulation, scaling potential. Characterization techniques essential for quality regulatory approval are systematically reviewed, followed applications across oncology, gene delivery, neurology, infectious disease treatment, supported recent experimental evidence. While magnetoliposomes show remarkable versatility, clinical translation requires addressing biocompatibility concerns, manufacturing scalability, hurdles. Integration artificial intelligence, organ-on-chip technologies, personalized medicine will likely accelerate development toward reality, potentially revolutionizing treatment paradigms complex diseases tailored interventions.

Language: Английский

Citations

0
A mathematical phase field model predicts superparamagnetic nanoparticle accelerated fusion of HeLa spheroids for field guided biofabrication DOI Creative Commons
Cristian F. Rodríguez, Valentina Quezada, Paula Guzmán-Sastoque

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: June 5, 2025

In vitro tissue models are crucial for regenerative medicine, drug discovery, and the reduction of animal testing. 3D bioprinting, particularly when utilizing magnetic manipulation cell spheroids, provides precise control over architecture. However, existing mathematical lack precision to capture interplay between biological dynamics forces during spheroid fusion. This study developed validated a novel model that simulates magnetically assisted fusion, taking into account migration, adhesion, effects external fields. The integrates principles mechanics, fluid dynamics, magnetostatics, implemented in COMSOL Multiphysics. Experimental validation used HeLa spheroids bioprinted with superparamagnetic iron oxide nanoparticles (SPIONs). Spheroid fusion was monitored without an field using confocal microscopy. Rigorous statistical analysis (MAE, RMSE, MAPE, R², Chi-Square, Bland-Altman, variance-weighted metrics) evaluate performance. accurately predicted accelerated under manipulation, reducing time from approximately 7 days (without field) 2 days. High R² values (> 0.99 two-spheroid > 0.97 multi-spheroid systems) narrow confidence intervals demonstrated strong agreement simulation experiment. Increased system complexity introduced slightly higher error variability, but maintained robust predictive capabilities. disassembly observed four-spheroid case, highlighting complex cellular reorganization. validated, high-precision represents significant advancement engineering, providing powerful tool optimizing bioprinting protocols, designing constructs, advancing development. breakthrough has implications medicine discovery while also importance addressing nanoparticle safety concerns.

Language: Английский

Citations

0