Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from the phytopathogenic fungus Lasiodiplodia pseudotheobromae

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

In this study, we isolated a novel mycovirus from Lasiodiplodia pseudotheobromae strain YY-1, which named “Lasiodiplodia pseudotheobromae Mycovirus 1” (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 ORF2). ORF1 encodes putative protein 316 amino acids with molecular weight 34.1 kDa, shares similarity the coat proteins several mycoviruses. ORF2 561 63.2 conserved RNA-dependent RNA polymerase (RdRp) domain. A BLASTp results show that RdRp highest non-segmented dsRNA viruses. Multiple sequence alignment phylogenetic analysis indicate new member proposed genus “Unirnavirus”. It first report L. pseudotheobromae.

Language: Английский

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Identification and management of a novel Danshen leaf anthracnose caused by Colletotrichum karstii in Salvia miltiorrhiza Bunge in China

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 4, 2025

Danshen ( Salvia miltiorrhiza Bunge), a member of the genus within Lamiaceae family, holds significant economic and medicinal value. Regrettably, emergence novel leaf anthracnose in 2020 has significantly impacted its cultivation, leading to decreased yield compromised quality. This newly identified pathogen was meticulously isolated from affected leaves, employing meticulous single conidia isolation techniques. Subsequent confirmation pathogenicity achieved through strict adherence Koch’s postulates. To ensure precise identification, morphological characteristics were supplemented with tandem sequence analysis targeting rDNA internal transcribed spacer ITS ), β-tubulin TUB histone His3 ) regions. Combining molecular biology techniques observation postulates, conclusively as Colletotrichum karstii . Further investigations focused on understanding environmental factors influencing mycelial growth sporulation pathogen. The optimum temperature for C.karstii is 25°C, suitable light conditions are 12h light/12h dark or 24h dark, pH 5 9. Utilizing BIOLOG phenotypic technique, metabolic utilization carbon nitrogen sources by assessed across different temperatures (20°C, 30°C). Results indicated highest rates at particularly arbutin L-tryptophan. Lastly, efficacy 15 chemical fungicides six botanical fungiticide against C. evaluated vitro, revealing fluazinam most potent inhibitor EC 50 0.0725 mg/mL mycelium 0.0378 spore germination, respectively. 1 % osthole emulsion water found have strongest inhibitory effect mycelium, an EC50 value 4.8984 µg/mL. Spore germination strongly inhibited 80 ethylicin EC, which had 0.5541 study represents first documentation causative agent anthrax Danshen, underscoring significance these findings agricultural management disease control strategies.

Language: Английский

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Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from the phytopathogenic fungus Lasiodiplodia pseudotheobromae

Archives of Virology, Journal Year: 2025, Volume and Issue: 170(4)

Published: March 8, 2025

Language: Английский

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Identification of a novel Gammapartitivirus associated with endophytic Fusarium oxysporum through transcriptome dataset mining

VirusDisease, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

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