The Induction of L-lysine-α-Oxidase from Trichoderma Harzianum Rifai by Metabolic Products of Brevibacterium sp. and the Improvement of Its Isolation and Purification Techniques

Avicenna Journal of Medical Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Background: The enzyme L-lysine-α-oxidase from Trichoderma harzianum Rifai is a promising anticancer, antifungal and antibacterial agent. Intensive exploring of its physico-chemical properties possible ways application requires sufficient amounts the protein which in turn depends on good techniques cultivation micro-organism producer, soft isolation purification, storage. Methods: An improved method has been suggested for purification enzyme. A specific combination column sorbents was adapted gradient elution with sodium chloride applied to elevate yield inductive influence Metabolic Products (MP) Brevibacterium species, along fungal metabolites Ulocladium sp. tested. activity assay based detection oxidized dimethylbenzidine peroxidase reaction coupled an reaction. Some were additionally explored. Results: upgraded technique resulted about 79%. All strains proved be potent enhancers concomitant activities. induced appeared less but more thermostable. Possible scopes modified are discussed. Phosphate buffer solution (pH=5.6) best one long-term storage Conclusion: significant inducing effect MP detected, have improved.

Language: Английский

Comprehensive insight into exploring the potential of microbial enzymes in cancer therapy: Progress, challenges, and opportunities: A review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134535 - 134535

Published: Aug. 5, 2024

Language: Английский