Evaluation of the Synergistic Activity of Antimicrobial Peptidomimetics or Colistin Sulphate with Conventional Antifungals Against Yeasts of Medical Importance DOI Creative Commons
Shyam Kumar Mishra, Rajesh Kuppusamy, Christina Q. Nguyen

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 370 - 370

Published: May 12, 2025

With rising multidrug-resistant yeast pathogens, conventional antifungals are becoming less effective, urging the need for adjuvants that enhance their activity at lower doses. This study evaluated synergistic of antimicrobial peptidomimetics (TM8 and RK758) or colistin sulphate in combination with against Candida albicans, C. tropicalis, parapsilosis, Meyerozyma guilliermondii, Nakaseomyces glabratus, Pichia kudriavzevii Kluyveromyces marxianus, Candidozyma auris using checkerboard microdilution test. RK758 was fluconazole 78% isolates, remaining 22% isolates still showing partial synergy; it showed synergy amphotericin B 56% caspofungin, exhibited either synergy. TM8 44% (with another 44%) 67% caspofungin isolates. Colistin No antagonism observed any combinations. Additionally, a time-kill assay further demonstrated between RK758. The effects these on cell membrane integrity were an ergosterol binding assay, supported by SYTOX Green cellular leakage assays, both indicating lytic effect. These results suggest can synergise antifungals, offering potential strategy therapy infections. likely plays role interaction thereby enhancing activities compounds sub-MIC levels.

Language: Английский

A cell wall-targeting urease-derived peptide as a potential antifungal agent against Candida species DOI Creative Commons
Ana Paula A. Perin, Júlia Catarina Vieira Reuwsaat, Heryk Motta

et al.

Current Research in Microbial Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 100399 - 100399

Published: May 1, 2025

Language: Английский

Citations

0
Evaluation of the Synergistic Activity of Antimicrobial Peptidomimetics or Colistin Sulphate with Conventional Antifungals Against Yeasts of Medical Importance DOI Creative Commons
Shyam Kumar Mishra, Rajesh Kuppusamy, Christina Q. Nguyen

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 370 - 370

Published: May 12, 2025

With rising multidrug-resistant yeast pathogens, conventional antifungals are becoming less effective, urging the need for adjuvants that enhance their activity at lower doses. This study evaluated synergistic of antimicrobial peptidomimetics (TM8 and RK758) or colistin sulphate in combination with against Candida albicans, C. tropicalis, parapsilosis, Meyerozyma guilliermondii, Nakaseomyces glabratus, Pichia kudriavzevii Kluyveromyces marxianus, Candidozyma auris using checkerboard microdilution test. RK758 was fluconazole 78% isolates, remaining 22% isolates still showing partial synergy; it showed synergy amphotericin B 56% caspofungin, exhibited either synergy. TM8 44% (with another 44%) 67% caspofungin isolates. Colistin No antagonism observed any combinations. Additionally, a time-kill assay further demonstrated between RK758. The effects these on cell membrane integrity were an ergosterol binding assay, supported by SYTOX Green cellular leakage assays, both indicating lytic effect. These results suggest can synergise antifungals, offering potential strategy therapy infections. likely plays role interaction thereby enhancing activities compounds sub-MIC levels.

Language: Английский

Citations

0