What makes Yarrowia lipolytica well suited for industry?

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(2), P. 242 - 254

Published: Aug. 6, 2022

Selection of the most appropriate microorganism is one key aspects for industrial success microbial bioprocesses.Yarrowia lipolytica has gained interest as a chassis strain in academia and industry because its capacity to make products at high yields, use broad range substrates, be genetically amenable.Y. many features that are desired an scale, such safety, robustness, efficient stable genetic modifications, variety ability grow very cell density.To further improve Y. lipolytica, some characteristics must improved through metabolic engineering, oxygen requirement, byproduct formation, excessive foam synthesis. Yarrowia possesses natural engineered traits it good host bioproduction chemicals, fuels, foods, pharmaceuticals. In recent years, academic researchers have assessed potential, developed synthetic biology techniques, features, scaled processes, identified limitations. Both publications patents related shown drastic increase during past decade. Here, we discuss this yeast suitable remaining challenges wider large scale. We present evidence herein shows importance potential may soon preferred choices industry. Industrial biotechnology (white biotechnology) includes application enzymes, extracts, or whole microorganisms processes lead manufacture wide products, including food ingredients, materials, pharmaceuticals [1.Frazzetto G. White biotechnology.EMBO Rep. 2003; 4: 835-837Crossref PubMed Scopus (63) Google Scholar,2.Heux S. et al.White biotechnology: state art strategies development biocatalysts biorefining.Biotechnol. Adv. 2015; 33: 1653-1670Crossref (71) Scholar]. Compared with chemical often more sustainable environmentally friendly enables specificity reactivity difficult achieve otherwise [3.Soetaert W. Vandamme E. The impact biotechnology.Biotechnol. J. 2006; 1: 756-769Crossref (81) two decades, along advances engineering biology, delivered innovations chemical, textile, food, packaging, healthcare sectors [4.Jullesson D. al.Impact on production fine chemicals.Biotechnol. 1395-1402Crossref (166) One keys bioprocess (see Glossary) selection microorganism. should consider their intermediates, consume substates, resist toxicity intermediates and/or final products. addition, important wealth knowledge organism's physiology metabolism well degree techniques [5.Keasling J.D. Synthetic tools engineering.Metab. Eng. 2012; 14: 189-195Crossref (340) A given can also by optimization fermentation conditions evolutionary adaptation Scholar, 6.Sopko R. al.Mapping pathways phenotypes systematic gene overexpression.Mol. Cell. 21: 319-330Abstract Full Text PDF (518) 7.Förster A.H. Gescher Metabolic Escherichia coli mixed-acid end products.Front. Bioeng. Biotechnol. 2014; 2: 16PubMed been considered nonconventional due distinctive genome structure relatively phylogenetic distance other yeasts while sharing common properties higher eukaryotes [8.Barth Gaillardin C. lipolytica.in: Wolf K. Nonconventional Yeasts Biotechnology: Handbook. Springer-Verlag, 1996: 313-388Crossref This was originally isolated from lipid-rich protein-rich environments fermented dairy (cheese, yogurt), meat, poultry, wastes sewage oil-polluted [9.Beopoulos A. al.Yarrowia lipolytica: model tool understand mechanisms implicated lipid accumulation.Biochimie. 2009; 91: 692-696Crossref (216) Since isolation, used organic acids heterologous proteins bioremediation oil-contaminated soil water [10.Nicaud J.-M. lipolytica.Yeast. 29: 409-418Crossref (208) Scholar,11.Madzak achievements protein expression pathway engineering.Appl. Microbiol. 99: 4559-4577Crossref (167) Some unique initially drew attention accumulate lipids, dimorphism (with both pseudo-hyphae forms) Scholar,12.Abdel-Mawgoud A.M. al.Metabolic lipolytica.Metab. 2018; 50: 192-208Crossref (126) Scholar], degrade hydrophobic carbon sources (fatty acids, triglycerides, alkanes, alkenes, etc.) [13.Barth Physiology genetics dimorphic fungus lipolytica.FEMS Rev. 1997; 19: 219-237Crossref Scholar,14.Lazar Z. al.Holistic approaches lipolytica.Trends 36: 1157-1170Abstract (86) 1990s, involving increased rapidly parallel early availability whole-genome sequence encouraged new research groups work microbe. Together manipulation, number published articles grown exponentially, Figure 1. academia, noticed yeast, (Figure 2). briefly explored patent landscape found 4536 international containing keywords 'Yarrowia lipolytica' As expected, significantly since 2001, alongside which reflect increasing organism host. Examples made described Box 1 2 Survey industry).Box 1Y. commercial industry(i)Polyunsaturated fatty (PUFAs), eicosapentaenoic acid (EPA, 20:5, n-3) docosahexaenoic (DHA, 22:6, n-3), known long-chain omega-3 global market valued US$2.49 billion 2019 [70.Xie al.Sustainable source metabolically fundamental production.Appl. 1599-1610Crossref (149) E.I. DuPont de Nemours Company strategy expressed Δ6 desaturase, C18/20 elongase, Δ5 Δ17 enabled up 40% EPA [71.Maccol, D.J. al. US Holding LLC. Mortierella alpina C16/18 elongase. US7470532B2.Google 72.Yadav, N.S. EI Du Pont Co. Δ12 desaturases altering leveles polyunsaturated oleaginous yeast. US7504259B2.Google 73.Zhu Q. al.Cohen Ratledge C.B.T.-S.C.O. 3 - Engineering Oleaginous Yeast Production Omega-3 Fatty Acids. 2nd ed. AOCS Press, 2010: 51-73Google enhanced eliminating competitive introducing several copies crucial reaching approximately 25% dry weight 50% methyl ester Scholar,74.Hong, S.P. Expression caleosin recombinant oil content therein. WO2012162368A1.Google commercialize produced New Harvest oil, Verlasso salmon.(ii)Skotan SA started single-cell (edible human animals [75.Bornscheuer U.T. fourth wave biocatalysis approaching.Philos. Trans. Soc. Math. Phys. Sci. 376: 1-7Google Scholar]) waste glycerol registered feed product EU [15.Groenewald M. safety assessment great potential.Crit. 40: 187-206Crossref (325) Microbial enzymes lipases already own US$425 million 2018 [76.Chandra P. al.Microbial applications: comprehensive review.Microb. Cell Fact. 2020; 169Crossref (315) Commercial now available, phospholipase enzyme Lipomod 833L2 Biocatalysts Ltd., lipase obtained LIP2 overexpression Mayoly, α-glucosidase OXY2810 Oxyrane [44.Madzak biotechnological review major innovations.Mol. 60: 621-635Crossref (73) Scholar,77.Madzak strains how biodiversity could contribute factories improvement.J. Fungi. 2021; 7: 548Crossref (42) Scholar].(iii)Citric widely additives, preservatives, anticoagulants, antimicrobial agents, so forth [78.Cavallo citric production.FEMS Res. 2017; 17: fox084Crossref (74) volume greater than tons, value estimated reach US$6.28 2030 [79.Market Research Future Citric Acid Market Report: Information Form (Anhydrous Liquid), Function (Acidulant, Antioxidant, Preservative, Flavouring Agent), Application [Food & Beverages (Beverages; Bakery Confectionery; Sweet Savoury Snacks; Soups, Sauces, Dressings, RTE RTC Meals, Others), Pharmaceuticals Nutraceuticals, Personal Care, Others] Region (North America, Europe, Asia-Pacific, RoW)—Forecast till 2030.2021Google Due easy cultivation, conversion rate, tolerance concentrations, proposed alternative producer Aspergillus niger Scholar,80.Börekçi B.S. al.Citric yeasts: overview.Eurobiotech 5: 79-91Crossref (12) Several companies, DSM, Akad Wissenschaften DDR, OrganoBalance GmbH, 40 Scholar].(iv)Carotenoids applications processing, animal feed, pharmaceutical, cosmetics industries. There enormous market, US$1.57 2022 valuation US$2.09 2027 [81.Market Data Forecast Global Carotenoids Segmented Type (Astaxanthin, Canthaxanthin, Lutein, Beta-Carotene, Lycopene, Zeaxanthin), (Food Beverages, Dietary Supplements, Animal Feed others), By Sources (Natural Synthetic) Regional Analysis Asia Pacific, Latin Middle East Africa) Industry Analysis, Size, Share, Growth, Trends, (2022 – 2027).2022Google carotenoids numerous filed Company, Amyris, Microbia Inc., among others [82.Royer, DSM IP Assets BV. terpenoids. US20180148697A1.Google 83.Farrell, Acetyl transferase producing carotenoids. US10865392B2.Google 84.Sharp, P.L. Carotenoid US8846374B2.Google 85.Gardner, T.S. Amyris Inc. acetyl-coenzymeA derived isoprenoids. US8603800B2.Google 86.Bailey, R.B. fungi. US9297031B2.Google (now part DSM) brought carotenoid pilot scales, GRAS self-affirmation demonstrated β-carotene Scholar].Box 2Survey industryAlthough opinion publications, those obtain. therefore conducted survey companies working find out what they think about consisted statements 'ranked questions' few 'open where answer typed. answered ten different scales (from lab scale).The first question' target IA). When considering 'agree' 'mostly agree' answers, terpenoids, were best targets agreement 100%, 80%, 73%, respectively. Interestingly, responses 'Which promising near future?' diverse, amino proteins, sugar alcohols mentioned, interests companies.A second set assess IB). Overall, there ('agree' agree') fact density cultivation (100%), robustness (70%), substrate utilization (80%), whereas less positive answers regard downstream scaling up, 30%, 60%, opinions, respectively, 'neutral' disagree'.The third investigated need studies, tools, IC). Ninety percent agree still needs tools. Eighty-two recognized deepening our understanding behavior bioreactors.These industries strains, being wild-type (nonengineered) strains. anonymous response stated 'Y. strong body knowledge, regulatory approvals prior (e.g., short list do not full before manufacture/sale EU), accelerates R D manufacturing timelines'.Figure ISurvey using lipolytica.Show caption(A) Agreement disagreement produce (B) presence lipolytica. (C) fields study lipolytica.View Large Image ViewerDownload Hi-res image Download (PPT) (i)Polyunsaturated Although scale). companies. disagree'. bioreactors. These timelines'. popularity goes beyond choosing workhorse, but makes appealing bioproduction? try question describing ideal fit biomanufacturing, current research, yet overcome better Traditionally, polyols. greatly expanded, 3. divert flux toward acetyl coenzyme turned into producers terpenes lipid-derived metabolites shikimate pentose phosphate pathway) demonstrated. compounds these cosmetics, pharmaceuticals, materials 3). Safety critical issues implementation applications, especially intended consumption. 'safe-to-use organism', granted (generally regarded safe) status acid, erythritol, FDA

Language: Английский

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YaliCMulti and YaliHMulti: Stable, efficient multi-copy integration tools for engineering Yarrowia lipolytica

Metabolic Engineering, Journal Year: 2024, Volume and Issue: 82, P. 29 - 40

Published: Jan. 14, 2024

Language: Английский

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Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies

Synthetic and Systems Biotechnology, Journal Year: 2022, Volume and Issue: 7(2), P. 689 - 704

Published: Feb. 18, 2022

The global market demand for natural astaxanthin is rapidly increasing owing to its safety, the potential health benefits, and diverse applications in food pharmaceutical industries. major native producers of on industrial scale are alga

Language: Английский

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Yarrowia lipolytica as an Alternative and Valuable Source of Nutritional and Bioactive Compounds for Humans

Molecules, Journal Year: 2022, Volume and Issue: 27(7), P. 2300 - 2300

Published: April 1, 2022

Yarrowia lipolytica, an oleagineous species of yeast, is a carrier various important nutrients. The biomass this yeast extensive source protein, exogenous amino acids, bioavailable essenctial trace minerals, and lipid compounds as mainly unsaturated fatty acids. also contains B vitamins, including vitamin B12, many other bioactive components. Therefore, Y. lipolytica can be used in food supplements for humans safe nutritional additives maintaining the homeostasis organism, vegans vegetarians, athletes, people after recovery, at risk deficiencies.

Language: Английский

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Advancing Yarrowia lipolytica as a superior biomanufacturing platform by tuning gene expression using promoter engineering

Bioresource Technology, Journal Year: 2022, Volume and Issue: 347, P. 126717 - 126717

Published: Jan. 11, 2022

Language: Английский

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Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 65, P. 108128 - 108128

Published: March 13, 2023

Language: Английский

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Exploiting synthetic biology platforms for enhanced biosynthesis of natural products in Yarrowia lipolytica

Bioresource Technology, Journal Year: 2024, Volume and Issue: 399, P. 130614 - 130614

Published: March 19, 2024

Language: Английский

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Construction of Yarrowia lipolytica for degradation of low-density polyethylene

Process Safety and Environmental Protection, Journal Year: 2025, Volume and Issue: unknown, P. 106818 - 106818

Published: Jan. 1, 2025

Language: Английский

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Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review

Biotechnology for Biofuels and Bioproducts, Journal Year: 2022, Volume and Issue: 15(1)

Published: April 4, 2022

Abstract Biodiesel is a renewable fuel that can be produced from range of organic and feedstock including fresh or vegetable oils, animal fats, oilseed plants. In recent years, the lignin-based aromatic wastes, such as various waste polymers agriculture, dye wastewater textile industry, have attracted much attention in academia, which uniquely selected potential for biodiesel product converted by yeast cell factory technology. This current investigation indicated highest percentage lipid accumulation achieved high 47.25% an oleaginous strain, Meyerozyma caribbica SSA1654, isolated wood-feeding termite gut system, where its synthetic oil conversion ability reach up to 0.08 (g/l/h) fatty acid composition cells represents over 95% total acids are similar oils. Clearly, use yeasts, termites, synthesizing lipids aromatics clean, efficient, competitive path achieve "a sustainable development" towards production. However, lacking potent yeasts transform aromatics, unknown metabolic regulation mechanism presented natural fundamental challenge we face development. Under this scope, review has proposed novel concept approach strategy utilization convert wastes substrate transformation. Therefore, screening robust strain(s) system with set desirable specific tolerance characteristics essential. addition, reconstruct pathway/network maximize transformation rate applications “omics” technologies biology approach, work agenda will also include analyze genome characteristics, develop new base mutation gene editing technology, well clarify influence insertion position compounds other biosynthetic pathways industrial chassis on expressional level stability. With these unique designs running advanced biotech approaches, pathway using developed potentially constructed, integrated optimized, suggesting hypothesis utilizing technically promising applicable near future.

Language: Английский

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Biotechnological production of sugar-alcohols: Focus on Yarrowia lipolytica and edible/medicinal mushrooms

Process Biochemistry, Journal Year: 2022, Volume and Issue: 124, P. 113 - 131

Published: Nov. 19, 2022

Language: Английский

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