Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase DOI
Mustansir Abbas, Uroosa Ejaz,

Maryam Shafique

et al.

Journal of Basic Microbiology, Journal Year: 2023, Volume and Issue: 63(7), P. 722 - 733

Published: March 1, 2023

Abstract Sugarcane bagasse (SB) is a promising source of appreciable quantities fermentable sugars. However, the presence lignin hinders utilization these carbohydrates and hence pretreatment to remove necessarily carried out. Here, biological method was synchronized with production thermostable cellulase using SB as raw material. Initially, fermented by laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) titer 1758 IU mL −1 obtained. Investigations nine factors affecting through Plackett Burman design improved titers 6539 . Five (incubation period, concentration CuSO 4 , temperature, moisture content, particle size) were found significant which optimized Central Composite leading an improvement in ~5 folds (8841 ). Biologically pretreated thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 cellulase. Delignification cellulose affirmed structural analysis FTIR SEM. The process higher SSF minimum use corrosive chemicals.

Language: Английский

Green synthesis of NiO NPs for metagenome-derived laccase stabilization: Detoxifying pollutants and wastes DOI
Shohreh Ariaeenejad, Mahmood Barani, Mina Sarani

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 266, P. 130986 - 130986

Published: March 18, 2024

Language: Английский

Citations

7
Microbial Immobilized Enzyme Biocatalysts for Multipollutant Mitigation: Harnessing Nature’s Toolkit for Environmental Sustainability DOI Open Access
Mohamed Abdel‐Hamid, Hazim O. Khalifa,

H. Yoon

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8616 - 8616

Published: Aug. 7, 2024

The ever-increasing presence of micropollutants necessitates the development environmentally friendly bioremediation strategies. Inspired by remarkable versatility and potent catalytic activities microbial enzymes, researchers are exploring their application as biocatalysts for innovative environmental cleanup solutions. Microbial enzymes offer substrate specificity, biodegradability, capacity to degrade a wide array pollutants, positioning them powerful tools bioremediation. However, practical applications often hindered limitations in enzyme stability reusability. Enzyme immobilization techniques have emerged transformative strategies, enhancing reusability anchoring onto inert or activated supports. These improvements lead more efficient pollutant degradation cost-effective processes. This review delves into diverse methods, showcasing success degrading various including pharmaceuticals, dyes, pesticides, microplastics, industrial chemicals. By highlighting potential immobilized biocatalysts, this underscores significance achieving cleaner sustainable future through mitigation micropollutant contamination. Additionally, research directions areas such engineering machine learning hold immense promise further broadening capabilities optimizing cleanup.

Language: Английский

Citations

7
Exploring laccase: a sustainable enzymatic solution for the paper recycling domain DOI
Sheetal Pandey, Sarika Gupta

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 11, 2024

Language: Английский

Citations

6
Construction of bacterial laccase displayed on the microbial surface for ultrasensitive biosensing of phenolic pollutants with nanohybrids-enhanced performance DOI
Yanfang Zhao, Jing Yang, Yuqing Wu

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 452, P. 131265 - 131265

Published: March 23, 2023

Language: Английский

Citations

16
Recent Theoretical Insights into the Oxidative Degradation of Biopolymers and Plastics by Metalloenzymes DOI Open Access
Anna Rovaletti, Luca De Gioia,

Piercarlo Fantucci

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6368 - 6368

Published: March 28, 2023

Molecular modeling techniques have become indispensable in many fields of molecular sciences which the details related to mechanisms and reactivity need be studied at an atomistic level. This review article provides a collection computational works on topic enormous interest urgent relevance: properties metalloenzymes involved degradation valorization natural biopolymers synthetic plastics basis both circular biofuel production bioremediation strategies. In particular, we will focus lytic polysaccharide monooxygenase, laccases, various heme peroxidases processing polysaccharides, lignins, rubbers, some polymers. Special attention dedicated interaction between these enzymes their substrate different levels theory, starting from classical docking dynamics up based quantum chemistry.

Language: Английский

Citations

16
Biochemical Characteristics of Laccases and Their Practical Application in the Removal of Xenobiotics from Water DOI Creative Commons
Agnieszka Gałązka, Urszula Jankiewicz, Andrzej Szczepkowski

et al.

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(7), P. 4394 - 4394

Published: March 30, 2023

The rapid growth of the human population in recent decades has resulted intensive development various industries, urban agglomerations and increased production medicines for animals humans, plant protection products fertilizers on an unprecedented scale. Intensive agriculture, expanding areas newly established industrial plants release huge amounts pollutants into environment, which, nature, are very slowly degraded or not decomposed, which leads to their accumulation water terrestrial ecosystems. Researchers scouring extremely contaminated environments identify organisms that have ability degrade resistant xenobiotics, such as PAHs, some pharmaceuticals, plasticizers dyes. These a potential source enzymes could be used bioremediation municipal wastewater. Great hopes pinned oxidoreductases, including laccase, called by green biocatalyst because end product oxidation wide range substrates this enzyme is other compounds, most often dimers, trimers polymers. Laccase immobilization techniques use systems together with adsorption separation found application enzymatic

Language: Английский

Citations

15
Laccase Engineering: Redox Potential Is Not the Only Activity-Determining Feature in the Metalloproteins DOI Creative Commons
Misha Ali, Priyanka Bhardwaj, Hassan Mubarak Ishqi

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(17), P. 6209 - 6209

Published: Aug. 23, 2023

Laccase, one of the metalloproteins, belongs to multicopper oxidase family. It oxidizes a wide range substrates and generates water as sole by-product. The engineering laccase is important broaden their industrial environmental applications. general assumption that low redox potential laccases principal obstacle, evidenced by activity towards certain substrates. Therefore, primary goal improve oxidation capability, thereby increasing potential. Even though some determinants are known, it still not entirely clear how enhance its However, active site has additional characteristics regulate enzymes’ specificity. These include electrostatic hydrophobic environment substrate binding pocket, steric effect at site, orientation with respect T1 laccase. In this review, these features will be discussed highlight importance target for future engineering.

Language: Английский

Citations

14
Advances and future perspectives in biotechnological and bioconversional of dates byproducts DOI Creative Commons
Khalid Hamid Musa, Ahmed A. M. Elnour

Journal of Agriculture and Food Research, Journal Year: 2024, Volume and Issue: 16, P. 101145 - 101145

Published: April 13, 2024

Date production poses significant environmental and economic problems. Conventional waste management methods are insufficient to address these issues. However, microorganisms can convert date into valuable products, thus making bioconversion a sustainable solution. This study investigated how processes manage byproducts, focusing on producing beneficial compounds nutrients in different industries. In addition, it emphasizes the importance of protecting environment managing responsibly data industry. studies recent research using fungi bacteria bioactive compounds, enzymes, polysaccharides. It determines whether products be used sectors, such as food, pharmaceuticals, cosmetics. examines for energy recycling materials biogas, electricity, biofuels. Bioconversion transform resources, thereby benefiting economy environment. Research has demonstrated successful antioxidants, polysaccharides from waste, with applications multiple Repurposing food useable resource reduces opens up new opportunities. By leveraging capabilities microorganisms, converted contributing preservation advancement. Improving biotechnology hone make them cheaper is essential. To maximize potential bioconversion, concerted development endeavors Priority should given refining processes, innovating addressing challenges associated implementation regulation, especially developing nations. Researchers, industries, policymakers must collaborate promote through bioconversion.

Language: Английский

Citations

5
Isolation, Screening and Morphological characterization of Laccase producing fungi DOI Open Access

Bhuneshwari Nayak,

Rachana Choudhary,

M. G. Roymon

et al.

International Research Journal on Advanced Science Hub, Journal Year: 2022, Volume and Issue: 4(02), P. 38 - 43

Published: Feb. 28, 2022

Research was carried out on the production of extracellular laccase enzyme by fungi isolated from different lignocellulolytic waste soils within Bhilai- Durg region Chhattisgarh, India. Soil samples were collected lignocel- lulolytic such as flower, citrus peel and paddy straw soil immediately transported to laboratory for analysis. Fungal colony serial dilution method potato dextrose agar. A total 49 fungal cultures samples. Isolated culture screened pro- duction in guaiacol containing agar plate. Laccase producing morphological identified Agharkar Institute Pune. The isolates Scytalidium lignicola, Humicola fuscoatra, Fusarium sp., Paecilomyces Curvularia Fusar- ium Aspergillus niger, Chaetomium Penicillium sp. And Histoplasma capsulatum

Language: Английский

Citations

19
Aflatoxin B1 Degradation by Ery4 Laccase: From In Vitro to Contaminated Corn DOI Creative Commons
Martina Loi,

Silvana De Leonardis,

Biancamaria Ciasca

et al.

Toxins, Journal Year: 2023, Volume and Issue: 15(5), P. 310 - 310

Published: April 27, 2023

Aflatoxins (AFs) are toxic secondary metabolites produced by Aspergillus spp. and found in food feed as contaminants worldwide. Due to climate change, AFs occurrence is expected increase also western Europe. Therefore, ensure safety, it mandatory develop green technologies for reduction contaminated matrices. With this regard, enzymatic degradation an effective environmentally friendly approach under mild operational conditions with minor impact on the matrix. In work, Ery4 laccase, acetosyringone, ascorbic acid, dehydroascorbic acid were investigated vitro, then applied artificially corn AFB1 reduction. (0.1 µg/mL) was completely removed vitro reduced 26% corn. Several products detected UHPLC-HRMS likely corresponded AFQ1, epi-AFQ1, AFB1-diol, or AFB1dialehyde, AFB2a, AFM1. Protein content not altered treatment, while slightly higher levels of lipid peroxidation H2O2 detected. Although further studies needed improve reduce treatment corn, results study promising suggest that laccase can be effectively

Language: Английский

Citations

13