Recombinant protein expression in yeast DOI
Divya Rawat, Preeti Verma, Upasana Yadav

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 57 - 78

Published: Sept. 13, 2024

Language: Английский

Selection of appropriate probiotic yeasts for use in dairy products: a narrative review DOI Creative Commons

Sanaz Kazemi,

Aziz Homayouni Rad, Hossein Samadi Kafil

et al.

Food Production Processing and Nutrition, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 14, 2025

Abstract Yeasts, with a history of approximately 5,000 years in food and medicinal applications, play vital role various industries. The advantages these microorganisms include probiotic effects, phytate biodegradation, mycotoxin breakdown, adsorption capabilities. Traditionally, research on probiotics has primarily focused lactic acid bacteria bifidobacteria; however, there is growing global interest incorporating yeast as supplement. Notably, species such Saccharomyces , Candida Debaryomyces Yarrowia Kluyveromyces have been identified for their potential. These yeasts are commonly introduced into dairy products, including kefir, yogurt, kumis, cheese. However, the compatibility matrices, well factors influencing viability functionality, remains critical area study. Ongoing exploring techniques to enhance during processing, given that different strains may exhibit varying survival rates properties. Consequently, represent promising avenue augmenting health benefits products. This development prompts essential discussions regarding selecting suitable specific applications. paper comprehensively examines historical discovery, types, properties, applications It aims shed light effects while addressing challenges associated appropriate optimize enhancement Graphical

Language: Английский

Citations

3

Microbial Secondary Metabolites via Fermentation Approaches for Dietary Supplementation Formulations DOI Creative Commons
Alexandru Rusu, Monica Trif, João Miguel Rocha

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(16), P. 6020 - 6020

Published: Aug. 11, 2023

Food supplementation formulations refer to products that are designed provide additional nutrients the diet. Vitamins, dietary fibers, minerals and other functional compounds (such as antioxidants) concentrated in supplements. Specific amounts of given body through food supplements, these include well so-called non-essential such secondary plant bioactive components or microbial natural addition narrower sense. A significant social challenge represents how moderately use resources light growing world population. In terms economic production (especially natural) molecules, ways white biotechnology with various microorganisms have recently been intensively explored. current review relevant supplements substances (e.g., vitamins, amino acids, used their via fermentative biotechnological approaches briefly reviewed. Biotechnology plays a crucial role optimizing fermentation conditions maximize yield quality target compounds. Advantages ability renewable feedstocks, high yields, potential for cost-effective large-scale production. Additionally, it can be more environmentally friendly compared chemical synthesis, reduces reliance on petrochemicals minimizes waste generation. Educating consumers about benefits, safety, methods general is crucial. Providing clear accurate information science behind help address any concerns misconceptions may have.

Language: Английский

Citations

24

Microbial protein sources: A comprehensive review on the potential usage of fungi and cyanobacteria in sustainable food systems DOI
Nagarjuna Prakash Dalbanjan,

Manjunath P. Eelager,

Shivayogi S. Narasagoudr

et al.

Food and Humanity, Journal Year: 2024, Volume and Issue: 3, P. 100366 - 100366

Published: July 27, 2024

Language: Английский

Citations

16

Local Sources of Protein in Low- and Middle-Income Countries: How to Improve the Protein Quality? DOI Creative Commons

Nitya Vissamsetti,

Mackenzie Simon-Collins,

Sheryl Lin

et al.

Current Developments in Nutrition, Journal Year: 2023, Volume and Issue: 8, P. 102049 - 102049

Published: Dec. 21, 2023

Protein inadequacy is a major contributor to nutritional deficiencies and adverse health outcomes of populations in low- middle-income countries (LMICs). People LMICs often consume diet predominantly based on staple crops, such as cereals or starches, derive most their daily protein intakes from these sources. However, plant-based sources contain low levels indispensable amino acids (IAAs). Inadequate intake IAA comparison with requirements limiting factor that results deficiency, consequently the long-term stunting wasting. In addition, factors antinutrients can diminish digestion absorption. This review describes affect quality, reviews dietary patterns discusses traditional novel small- large-scale techniques improve quality plant for enhanced bioavailability digestibility an approach tackle malnutrition LMICs. The more accessible small-scale food-processing be implemented at home include soaking, cooking, germination, whereas many must industrial level autoclaving extrusion. Limitations considerations implement those locally are discussed. For instance, at-home processing cause loss nutrients contamination, limitations larger scale high energy requirements, costs, safety considerations. suggests combining approaches could local proteins, thereby address outcomes, particularly vulnerable population groups children, adolescents, elderly, pregnant lactating women.

Language: Английский

Citations

15

Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production DOI

Qingzi Lei,

Jun Ma, Guocheng Du

et al.

Food Research International, Journal Year: 2023, Volume and Issue: 170, P. 113017 - 113017

Published: May 20, 2023

Language: Английский

Citations

14

Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system DOI

Azadeh Eskandari,

Nima Ghahremani Nezhad,

Thean Chor Leow

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 40(1)

Published: Dec. 8, 2023

Language: Английский

Citations

14

Contribution of yeast and its biomass for the preparation of industrially essential materials: A boon to circular economy DOI
Atif Khurshid Wani, Farida Rahayu, Ita Yustina

et al.

Bioresource Technology Reports, Journal Year: 2023, Volume and Issue: 23, P. 101508 - 101508

Published: June 16, 2023

Language: Английский

Citations

11

Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome DOI Creative Commons
Hugh D. Goold, Heinrich Kroukamp, Paige E. Erpf

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 20, 2025

Abstract The Sc2.0 global consortium to design and construct a synthetic genome based on the Saccharomyces cerevisiae commenced in 2006, comprising 16 chromosomes new-to-nature tRNA neochromosome. In this paper we describe assembly debugging of 902,994-bp chromosome synXVI project. Application CRISPR D-BUGS protocol identified defective loci, which were modified improve sporulation recover wild-type like growth when grown glycerol as sole carbon source at 37˚C. LoxPsym sites inserted downstream dubious open reading frames impacted 5’ UTR genes required for optimal systematic cause growth. Based lessons learned from analysis defects , an in-silico redesign was performed, can be used blueprint future yeast designs. includes reduced PCR tag frequency, chunk megachunk termini, adjustments allocation loxPsym TAA stop codons ORFs. This provides roadmap into applications strategies non-yeast organisms.

Language: Английский

Citations

0

Comparative analysis of microbial diversity in various kombucha starter cultures in Malaysia using the random amplified polymorphic DNA (RAPD) approach DOI Open Access

Nur Syafiqah Syahmimie Augustine,

Eric Tzyy Jiann Chong, Carissa Wong

et al.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: Jan. 31, 2025

Kombucha is sweetened black tea fermented with symbiotic culture of bacteria and yeasts (SCOBY) has been widely consumed for its purported health benefits. The microbial consortium in kombucha dominated by such as Brettanomyces sp. Zygosaccharomyces sp., well acetic acid (AAB), Komagataeibacter Acetobacter However, the source SCOBY substrates used may affect diversity biochemical, flavour kombucha. Identifying population important use undefined starter cultures lead to variable metabolite production increase risk food pathogen contamination which can pose harm human health. This study aims isolate microbes from Malaysia future development a new specific species safe consumption Briefly, total 100 colonies were isolated nine selective plates. Differences colony morphology observed based on their colours, surface texture, elevation, margin. Their phenotypic genetic screened using microscopic examination, coupled catalase test random amplified polymorphic DNA (RAPD), respectively. Based data, 51% isolates showed under while rest bacteria. Additionally, 55.79% distinct banding profile patterns RAPD assessments. In conclusion, data this shows that there diverse different Malaysia, mainly predominated Gram-negative AAB Gram-positive yeasts. Identification at level be conducted future. By understanding microbiota kombucha, it contributes functional drink.

Language: Английский

Citations

0

Durum wheat nuclear factor Y (NF-Y) a subfamily: structure, phylogeny, and expression analysis in response to hormones and abiotic stresses DOI Creative Commons

Yosra Chouaibi,

Mohamed Taieb Bouteraa, Walid Ben Romdhane

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 14, 2025

Language: Английский

Citations

0