Photoreactivation Activities of Rad5, Rad16A and Rad16B Help Beauveria bassiana to Recover from Solar Ultraviolet Damage DOI Creative Commons

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(6), P. 420 - 420

Published: June 13, 2024

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair nucleotide excision of ultraviolet-induced DNA damage; however, their homologs have not yet been studied non-yeast fungi. fungus Beauveria bassiana, a deficiency Rad7 homolog, ortholog two Rad16 paralogs (Rad16A/B) instituted an ability to help insect-pathogenic recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were altered absence rad5, rad16A or rad16B, while severe defects growth conidiation caused by double deletion rad16B. Compared with wild-type complemented strains, mutants showed differentially reduced activities regarding resilience UVB-impaired conidia at 25 °C 12-h incubation regime visible light plus dark (L/D 3:9 h 5:7 for photoreactivation) full darkness (dark reactivation) mimicking natural nighttime. estimates median lethal dose LD50 L/D treatments revealed greater Rad16B than Rad16A additive either NER-dependent reactivation photorepair-dependent However, limited recovering low dose-impaired but unable impaired sublethal doses as did photoreactivation 5:7, unless night/dark time was doubled further prolonged. Therefore, anti-UV effects Rad5, B. bassiana depend primarily on are mechanistically distinct those yeast homologs.

Language: Английский

A negative melanin regulator, Mamrn, regulates thermo and UV tolerance via distinct mechanisms in Metarhizium DOI Creative Commons
Yanping Qin, Yuxian Xia

Microbiological Research, Journal Year: 2025, Volume and Issue: 297, P. 128190 - 128190

Published: April 22, 2025

Language: Английский

Citations

0
Rad6, a ubiquitin conjugator required for insect-pathogenic lifestyle, UV damage repair, and genomic expression of Beauveria bassiana DOI

Xin-Cheng Luo,

Lei Yu, Siyuan Xu

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127622 - 127622

Published: Jan. 18, 2024

Language: Английский

Citations

3
Comparative Roles of Rad4A and Rad4B in Photoprotection of Beauveria bassiana from Solar Ultraviolet Damage DOI Creative Commons
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(2), P. 154 - 154

Published: Jan. 23, 2023

The Rad4-Rad23-Rad33 complex plays an essential anti-ultraviolet (UV) role depending on nucleotide excision repair (NER) in budding yeast but has been rarely studied filamentous fungi, which possess two Rad4 paralogs (Rad4A/B) and orthologous Rad23 rely the photorepair of UV-induced DNA lesions, a distinct mechanism behind photoreactivation UV-impaired cells. Previously, nucleocytoplasmic shuttling proved to be highly efficient conidia inactivated by UVB, major component solar UV, due its interaction with Phr2 Beauveria bassiana, wide-spectrum insect mycopathogen lacking Rad33. Here, either Rad4A or Rad4B was proven localize exclusively nucleus interact Rad23, previously shown white collar protein WC2 as regulator photorepair-required photolyases (Phr1 Phr2) B. bassiana. Δrad4A mutant lost ~80% conidial UVB resistance ~50% activity UVB-inactivated 5 h light exposure. Intriguingly, reactivation rates UVB-impaired were observable only presence rad4A after dark incubation exceeding 24 h, implicating extant, infeasible, NER for field where night (dark) time is too short. Aside from strong anti-UVB role, played no other bassiana's lifecycle while functionally redundant. Our findings uncover that depends ascribed linked expands molecular basis underlying fungal adaptation UV irradiation Earth's surface.

Language: Английский

Citations

8
Co-Regulatory Roles of WC1 and WC2 in Asexual Development and Photoreactivation of Beauveria bassiana DOI Creative Commons
Siyuan Xu, Lei Yu,

Xin-Cheng Luo

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(3), P. 290 - 290

Published: Feb. 23, 2023

The white collar proteins WC1 and WC2 interact with each other to form a complex acting as well-known transcription regulator required for the operation of circadian clock in Neurospora, but their roles insect-pathogenic fungal lifecycles remain poorly understood. Here, we report that orthologs co-regulate conidiation capacity conidial resistance solar ultraviolet-B (UVB) irradiation Beauveria bassiana, after high activities photorepair UVB-induced DNA damages were elucidated previously insect mycopathogen, which features non-rhythmic capacity. yield, UVB resistance, photoreactivation rate UVB-impaired conidia greatly reduced null mutants wc1 wc2 compared control strains. However, many lifecycle-related phenotypes, except antioxidant response, rarely affected two mutants. Transcriptomic analysis revealed largely overlapping regulating gene networks. Most differentially expressed genes identified from (1380) (1001) co-downregulated (536) or co-upregulated (256) at similar levels, including several aerial photorepair. These findings expand molecular basis underlying adaptation UV offer novel insight into genome-wide co-regulatory B. bassiana's asexual development vivo against damage.

Language: Английский

Citations

8
UV-A Radiation Stimulates Tolerance against Fusarium oxysporum f. sp. lycopersici in Tomato Plants DOI Creative Commons
Yolanda González‐García,

Diego Iván Escobar-Hernández,

Adalberto Benavides‐Mendoza

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(4), P. 499 - 499

Published: April 17, 2023

UV-A radiation is a tool that has stimulant action in plants and can be used to induce benefits through the production of bioactive compounds synthesis secondary metabolites, among others. Particularly, its application could stimulate tolerance biotic stress caused by Fusarium oxysporum f. sp. lycopersici (Fol) tomato plants; for this reason, aim present study was evaluate ability against Fol. For this, crop established which two conditions supplementation with were tested on inoculated fungus The pathogen’s impact agronomic parameters, incidence severity, contents biomarkers, metabolites photosynthetic pigments determined. results show pathogen severity about 60%; therefore, fruit yield also negative (−31%). Moreover, significantly impacted content positive control increased H2O2 (+23.9%), malondialdehyde (+41.7%) proline (+54.8%). In contrast, decreased Fol (−35.5%), prevented effect plant/fruit yield. addition, biomarkers (−10.4% O2•−, −22% H2O2, −16% MDA), (+13.2% flavonoids, +35% anthocyanins) (+17% β-carotene, +12% yellow pigments, +19.8% total chlorophyll) pathogen. From obtained, it concluded good alternative means attack pathogens such as plants, without adverse consequences environment or crop.

Language: Английский

Citations

6
High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage DOI
Lei Yu, Siyuan Xu,

Xin-Cheng Luo

et al.

Journal of Photochemistry and Photobiology B Biology, Journal Year: 2024, Volume and Issue: 251, P. 112849 - 112849

Published: Jan. 23, 2024

Language: Английский

Citations

2
Recovery of insect-pathogenic fungi from solar UV damage: Molecular mechanisms and prospects DOI
Ming‐Guang Feng

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 59 - 82

Published: Jan. 1, 2024

Language: Английский

Citations

2
Divergent roles of Rad4 and Rad23 homologs in Metarhizium robertsii ’s resistance to solar ultraviolet damage DOI

Yilu Zhang,

Peng Han, Ke Zhang

et al.

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(9)

Published: Sept. 1, 2023

The anti-ultraviolet (UV) role of a Rad4-Rad23-Rad33 complex in budding yeast relies on nucleotide excision repair (NER), which is mechanistically distinct from photorepair DNA lesions generated under solar UV irradiation but remains poorly known filamentous fungi. Here, two nucleus-specific Rad4 paralogs (Rad4A and Rad4B) nucleocytoplasmic shuttling Rad23 ortholog are functionally characterized by multiple analyses their null mutants Metarhizium robertsii, an entomopathogenic fungus lacking Rad33. Rad4A was proven to interact with contribute significantly more conidial UVB resistance (90%) than (65%). Despite no other biological function, exhibited very high activity photoreactivation UVB-impaired/inactivated conidia 5-h light exposure due its interaction Rad10, anti-UV protein clarified previously have acquired similar through photolyase M. robertsii. NER or revealed lower reactivation rates moderately impaired after 24-h dark incubation hardly observable at the end 12-h incubation, suggesting infeasibility field where nighttime too short. Aside remarkable contribution resistance, had pleiotropic effect radial growth, aerial conidiation, antioxidant response, cell wall integrity activity. However, Rad4B proved redundant function. unveils essentiality for robertsii's fitness homolog's depending NER. IMPORTANCE Resilience ultraviolet (UV)-impaired cells crucial application fungal insecticides based formulated conidia. Anti-UV roles Rad4, Rad23, Rad33 rely upon (NER) yeast. Among robertsii Rad33, contributes interacts rather Rad4B. acquires severely inactivated another photorepair-required photolyase. either seemingly extant unfeasible conditions. has asexual cycle vitro Therefore, strong depends photoreactivation, unveiling scenario NER-reliant role.

Language: Английский

Citations

5
Rad2, Rad14 and Rad26 recover Metarhizium robertsii from solar UV damage through photoreactivation in vivo DOI
Peng Han,

Yilu Zhang,

Sheng‐Hua Ying

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127589 - 127589

Published: Dec. 23, 2023

Language: Английский

Citations

5
Efficient Photoreactivation of Solar UV‐InjuredMetarhizium robertsii by Rad1 and Rad10 Linked to DNA Photorepair‐Required Proteins DOI

Yi‐Lu Zhang,

Peng Han, Sheng‐Hua Ying

et al.

Photochemistry and Photobiology, Journal Year: 2022, Volume and Issue: 99(4), P. 1122 - 1130

Published: Nov. 28, 2022

Abstract Nucleotide excision repair (NER) of ultraviolet (UV)‐induced DNA lesions known as cyclobutane pyrimidine dimer (CPD) and (6–4)‐pyrimidine‐pyrimidone (6‐4PP) photoproducts depends on the activities multiple anti‐UV radiation (RAD) proteins in budding yeast. However, NER remains poorly filamentous fungi, whose are photorepaired by one or two photolyases, namely CPD‐specific Phr1 and/or 6‐4PP‐specific Phr2. Previously, white collar WC1 WC2 were proven to regulate expressions phr2 phr1 photorepair 6‐4PP CDP lesions, respectively, Metarhizium robertsii , a entomopathogenic‐phytoendophytic fungus. We report here high orthologous Rad1 Rad10 5‐h photoreactivation UVB‐injured UVB‐inactivated conidia but severely compromised capability their reactivating those via 24‐h dark incubation M. . The null mutants rad1 rad10 much more conidial UVB resistance than previous wc1 wc2 Multiple protein–protein (Rad1‐Rad10, Rad1‐WC2, Rad10‐Phr1, WC1‐Phr1/2 WC2‐Phr1/2) interactions detected suggest direct/indirect links Phr1/2 WC1/2 an importance for activities. Conclusively, photoreactivate UVB‐impaired through with photorepair‐required proteins.

Language: Английский

Citations

7