bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 15, 2024
Abstract
Root-associated
Helotiales
fungi
are
increasingly
recognized
as
beneficial
fungal
partners
promoting
plant
growth
under
nutrient-limited
conditions,
particularly
in
non-mycorrhizal
hosts,
lacking
the
ancestral
arbuscular
mycorrhizal
symbiosis.
However,
ecology
of
these
is
still
cryptic
relatively
few
lineages
have
been
successfully
cultivated
from
roots
for
further
study.
Here,
we
attempted
mass
isolation
root
endophytic
to
evaluate
recovery
known
plant-beneficial
using
a
tailored
culture-based
approach.
We
sampled
six
wild
species
Brassicaceae,
Caryophyllaceae,
and
Cyperaceae,
growing
alpine
soils.
isolated
602
endophytes
compared
this
culturable
diversity
with
one
observed
via
ITS2
metabarcoding.
Metabarcoding
revealed
that
taxa
dominated
communities,
43%
detected
also
represented
our
collection.
Accordingly,
most
collection
(53%)
were
Helotiales.
These
isolates,
some
P
solubilisation
potential,
belonged
primarily
three
Helotialean
clades
phylogenetically
related
growth-promoting
or
mycorrhizal-like
strains.
This
analysis
highlights
plants
reservoirs
root-endophytic
Helotiales,
isolates
obtained
promising
resource
explore
mechanisms
ecological
traits
fungi.
Soil Biology and Biochemistry,
Journal Year:
2024,
Volume and Issue:
191, P. 109363 - 109363
Published: Feb. 13, 2024
Fungi
contribute
to
plant
mineral
nutrition
through
diverse
symbiotic
mycorrhizal
associations.
Although
historically
most
attention
has
been
put
on
Glomeromycotina
fungi
establishing
the
ancestral
arbuscular
symbiosis,
other
fungal
lineages
have
developed
similar
but
evolutionarily
younger
associations
with
plants.
The
Helotiales,
a
largely
understudied
order,
is
emerging
as
key
lineage
in
fungus-mediated
nutrient
acquisition
by
Indeed,
these
can
form
ericoid
mycorrhizae
or
ectomycorrhizae
while
some
isolates
behave
so-called
"mycorrhizal-like"
capable
of
transferring
nutrients
their
hosts
non-symbiotic
way,
i.e.
without
forming
differentiated
cellular
structures
inside
roots,
and
proven
nutritional
reciprocity
from
plant.
Here,
we
explore
current
knowledge
about
phylogeny
plant-beneficial
Helotiales
cover
recent
advances
roles
they
play
nutrition,
highlighting
potential
alternatives
AM
symbiosis
for
uptake.
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(5), P. 329 - 329
Published: April 30, 2024
Climate
change
is
a
notable
challenge
for
agriculture
as
it
affects
crop
productivity
and
yield.
Increases
in
droughts,
salinity,
soil
degradation
are
some
of
the
major
consequences
climate
change.
The
use
microorganisms
has
emerged
an
alternative
to
mitigate
effects
Among
these
microorganisms,
dark
septate
endophytes
(DSEs)
have
garnered
increasing
attention
recent
years.
Dark
shown
capacity
mitigating
reducing
harmful
agriculture,
such
drought,
reduced
nutrient
availability
soil.
Various
studies
show
that
their
association
with
plants
helps
reduce
abiotic
stresses
increases
availability,
enabling
thrive
under
adverse
conditions.
In
this
study,
effect
DSEs
underlying
mechanisms
help
develop
higher
tolerance
were
reviewed.
Climate
change
is
a
big
challenge
for
agriculture
since
it
affects
crop
productivity
and
yield.
The
increase
of
droughts,
salinity,
soil
degradation
are
some
the
main
consequences
climate
change.
use
microorganisms
has
emerged
as
an
alternative
to
mitigate
them.
Among
these
microorganisms,
dark
septate
endophytes
(DSEs)
have
garnered
more
attention
in
recent
years.
Various
studies
show
that
their
association
with
plants
helps
reduce
harmful
effects
abiotic
stresses
increases
nutrient
availability,
allowing
thrive
under
adverse
conditions.
This
work
reviews
effect
DSEs
subjacent
mechanisms
will
help
develop
higher
tolerance
Agronomy,
Journal Year:
2024,
Volume and Issue:
14(8), P. 1801 - 1801
Published: Aug. 15, 2024
Dark
septate
endophytes
(DSE)
commonly
inhabit
the
root
systems
of
plants
that
flourish
in
heavy
metal-contaminated
soils.
Nevertheless,
there
is
limited
understanding
regarding
overall
response
medicinal
to
DSE
under
metal
stress.
The
present
study
utilized
a
non-sterile
pot
experiment
evaluate
beneficial
impacts
(Paraphoma
chlamydocopiosa,
Paraboeremia
selaginellae,
and
Paraphoma
radicina)
inoculation
on
Astragalus
mongholicus
cadmium
(Cd)
At
0
mg
Cd/kg
soil,
with
led
significant
increase
total
biomass
host
plant
by
up
34.0%.
Although
decreased
at
5
soil
10
increased
72.3%
after
inoculation.
height,
branch
number,
analyzer
development
(SPAD)
value,
were
not
affected
Cd
concentration
P.
chlamydocopiosa
selaginellae.
emerged
as
most
prominent
explanatory
variable
(44.2%)
for
growth
soil.
Inoculation
length
47.9%
74.1%,
selaginellae
indole-3-acetic
acid
(IAA)
content
103.6%
105.8%,
respectively,
Additionally,
radicina
was
preferred
calycosin-7-O-glucoside,
while
formononetin.
enhanced
accumulation
shoot,
whereas
exhibited
an
inhibitory
effect
system.
translocation
factor,
which
exceeded
one.
interaction
between
factors
significantly
contributed
growth.
improved
organic
carbon
reduced
available
nitrogen
content,
regardless
phosphorus
Importantly,
promote
performance
calycosin-7-O-glucoside
plant,
roots
These
data
comprehension
ecological
function
soils
demonstrated
potential
utility
strains
cultivating
plants.
Environmental Microbiology,
Journal Year:
2025,
Volume and Issue:
27(4)
Published: April 1, 2025
ABSTRACT
Root‐associated
Helotiales
fungi
are
increasingly
recognised
as
beneficial
fungal
partners
promoting
plant
growth
under
nutrient‐limited
conditions,
particularly,
in
non‐mycorrhizal
hosts
lacking
the
ancestral
arbuscular
mycorrhizal
symbiosis.
However,
ecology
of
these
is
still
cryptic
relatively
few
lineages
have
been
successfully
cultivated
from
roots
for
further
study.
Here,
we
attempted
mass
isolation
root
endophytic
to
evaluate
recovery
known
plant‐beneficial
using
a
tailored
culture‐based
approach.
We
sampled
six
wild
species
Brassicaceae,
Caryophyllaceae,
and
Cyperaceae,
growing
alpine
soils.
isolated
602
endophytes
compared
this
culturable
diversity
with
one
observed
via
ITS2
metabarcoding.
Metabarcoding
revealed
that
taxa
dominated
communities,
43%
detected
also
represented
our
collection.
Accordingly,
most
collection
(53%)
were
Helotiales.
These
isolates,
some
P
solubilisation
potential,
belonged
primarily
three
Helotialean
clades
phylogenetically
related
growth‐promoting
or
mycorrhizal‐like
strains.
This
analysis
highlights
plants
harbour
diverse
root‐endophytic
Helotiales,
isolates
obtained
promising
resource
explore
mechanisms
ecological
traits
fungi.
BMC Microbiology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: April 30, 2025
Abstract
Background
Plant-microbe
interactions
in
the
rhizosphere
and
endosphere
are
crucial
for
maintaining
plant
health
ecosystem
dynamics.
These
shaped
by
several
factors,
including
plant’s
developmental
stage,
domestication,
specific
root
compartments.
Different
cultivars
influence
unique
microbial
communities
secreting
exudates
that
either
support
beneficial
symbionts
or
inhibit
pathogens.
This
study
examined
community
structures
of
wild-type
finger
millet
five
domesticated
at
two
stages.
Results
Our
results
revealed
compartment,
domestication
significantly
root-associated
microbiomes.
Interestingly,
only
about
8%
core
microbiota
was
consistently
shared
between
soil
plants,
indicating
92%
shifted
dynamically
depending
on
type
stage.
Pseudomonadota
,
Actinomycedota
Bacteroidota
were
dominant
bacterial
phyla,
while
Ascomycota
Basidiomycota
primary
fungal
phyla
across
all
samples,
displaying
distinct
abundance
patterns.
Notably,
an
increase
correlated
with
a
reduction
.
The
most
significant
shifts
composition
occurred
during
flowering
primarily
driven
genus
Pseudomonas
findings
demonstrate
stages
recruitment
taxa
to
meet
needs,
particularly
various
selective
highlights
active
role
plants
shaping
their
microbiomes,
providing
insights
into
potential
manipulating
these
enhance
crop
productivity
sustainably.
Conclusion
indicate
both
host
stage
assembly
structure
microbiome.
Plant
compartments
can
selectively
recruit
from
associated
particular
compartment
involved.
deterministic
selection
pressures
exerted
growth
development
greatly
affect
communities.
has
important
implications
developing
sustainable
farming
practices,
reducing
reliance
chemical
fertilizers
pesticides,
enhancing
future
productivity.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(5), P. 531 - 531
Published: April 29, 2023
Dark
septate
endophytes
(DSEs)
can
improve
the
performance
of
host
plants
grown
in
heavy
metal-polluted
soils,
but
mechanism
is
still
unclear.
A
sand
culture
experiment
was
performed
to
investigate
effects
a
DSE
strain
(Exophiala
pisciphila)
on
maize
growth,
root
morphology,
and
cadmium
(Cd)
uptake
under
Cd
stress
at
different
concentrations
(0,
5,
10,
20
mg·kg−1).
The
results
indicated
that
significantly
improved
tolerance
maize,
causing
increases
biomass,
plant
height,
morphology
(length,
tips,
branch,
crossing
number);
enhancing
retention
roots
with
decrease
transfer
coefficient
plants;
increasing
proportion
cell
wall
by
16.0–25.6%.
In
addition,
changed
chemical
forms
roots,
resulting
decreases
proportions
pectates
protein-integrated
15.6–32.4%,
an
increase
insoluble
phosphate
33.3–83.3%.
correlation
analysis
revealed
positive
relationship
between
wall.
Therefore,
both
modifying
promoting
binding
walls
forming
lower
activity.
These
this
study
provide
comprehensive
evidence
for
mechanisms
which
colonization
enhances
subcellular
distribution
forms.
Frontiers in Microbiology,
Journal Year:
2023,
Volume and Issue:
14
Published: May 12, 2023
Knowledge
of
variations
in
abundant
and
rare
soil
microbial
communities
interactions
during
secondary
forest
succession
is
lacking.
Soil
samples
were
gathered
from
different
successional
stages
(grassland,
shrubland,
forest)
to
study
the
responses
bacterial
fungal
communities,
driving
factors
by
Illumina
sequencing
16S
ITS
rRNA
genes.
The
results
showed
that
α-diversities
(Shannon
index)
bacteria
fungi
revealed
no
significant
changes
succession,
but
increased
significantly
for
bacteria.
β-diversities
changed
succession.
Network
analysis
obvious
topological
properties
(nodes,
links,
average
degree)
networks
In
contrast,
these
higher
than
those
grassland
indicating
are
more
responsive
microorganisms.
Additionally,
greater
network
complexity
due
their
numbers
nodes
links.
keystone
species
differed
between
consisted
1
48
taxa
networks,
respectively.
TP
was
most
important
influencing
factor
communities.
Successional
plant
richness
had
influences
on
C:P,
SM
N:P
mainly
related
properties.
Our
indicates
respond
differently
Microbiology Spectrum,
Journal Year:
2023,
Volume and Issue:
11(5)
Published: Sept. 7, 2023
ABSTRACT
Ectomycorrhizal
(ECM)
fungi
and
dark
septate
endophytes
(DSEs)
can
both
form
a
symbiotic
relationship
with
the
same
host
plant.
However,
interactions
that
occur
among
these
two
types
of
their
co-hosts
are
largely
unknown.
Here,
we
investigated
ECM
fungus
Suillus
bovinus
,
DSE
Phialocephala
fortinii
co-host
Pinus
massoniana
.
We
used
scanning
electron
microscopy
optical
to
characterize
morphogenesis
symbionts
employed
ultra-high-performance
liquid
chromatography–tandem
mass
spectrometry
technique
assess
effects
fungal
inoculation
on
root
metabolome.
Under
pure
culture
conditions,
no
synergistic
or
antagonistic
were
observed
between
Phi.
S.
Generally,
simultaneously
colonize
P.
roots
without
affecting
each
other’s
processes.
locus
where
has
already
invaded
but
not
vice
versa,
which
may
be
due
physical
barrier
effect
mantle.
Both
significantly
promote
growth
they
have
N
K
uptake.
Metabolite
accumulation
patterns
in
inoculated
and/or
greatly
altered,
especially
respect
organic
acids,
flavonoids,
lipids,
phenolic
acids.
enhanced
flavonoid
biosynthesis,
whereas
dual-inoculation
treatments
mainly
induced
phenylpropanoid
biosynthesis.
These
findings
reveal
compatible
relationships
suggest
theoretical
basis
for
co-application
when
cultivating
seedlings.
IMPORTANCE
The
prevalence
ectomycorrhizal
wide
spectrum
tree
species
is
well
recognized.
In
this
study,
co-host,
appears
superior
microniche
competition,
different
metabolite
patterns.
This
first
study
revealing
morphological
metabolic
mechanisms
contribute
fungi,
DSEs,
co-host.