Root-associated Helotiales fungi: Overlooked players in plant nutrition

Soil Biology and Biochemistry, Journal Year: 2024, Volume and Issue: 191, P. 109363 - 109363

Published: Feb. 13, 2024

Fungi contribute to plant mineral nutrition through diverse symbiotic mycorrhizal associations. Although historically most attention has been put on Glomeromycotina fungi establishing the ancestral arbuscular symbiosis, other fungal lineages have developed similar but evolutionarily younger associations with plants. The Helotiales, a largely understudied order, is emerging as key lineage in fungus-mediated nutrient acquisition by Indeed, these can form ericoid mycorrhizae or ectomycorrhizae while some isolates behave so-called "mycorrhizal-like" capable of transferring nutrients their hosts non-symbiotic way, i.e. without forming differentiated cellular structures inside roots, and proven nutritional reciprocity from plant. Here, we explore current knowledge about phylogeny plant-beneficial Helotiales cover recent advances roles they play nutrition, highlighting potential alternatives AM symbiosis for uptake.

Language: Английский

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Importance of Dark Septate Endophytes in Agriculture in the Face of Climate Change

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(5), P. 329 - 329

Published: April 30, 2024

Climate change is a notable challenge for agriculture as it affects crop productivity and yield. Increases in droughts, salinity, soil degradation are some of the major consequences climate change. The use microorganisms has emerged an alternative to mitigate effects Among these microorganisms, dark septate endophytes (DSEs) have garnered increasing attention recent years. Dark shown capacity mitigating reducing harmful agriculture, such drought, reduced nutrient availability soil. Various studies show that their association with plants helps reduce abiotic stresses increases availability, enabling thrive under adverse conditions. In this study, effect DSEs underlying mechanisms help develop higher tolerance were reviewed.

Language: Английский

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Importance of Dark Septate Endophytes in Agriculture in the Face of Climate Change

Published: March 7, 2024

Climate change is a big challenge for agriculture since it affects crop productivity and yield. The increase of droughts, salinity, soil degradation are some the main consequences climate change. use microorganisms has emerged as an alternative to mitigate them. Among these microorganisms, dark septate endophytes (DSEs) have garnered more attention in recent years. Various studies show that their association with plants helps reduce harmful effects abiotic stresses increases nutrient availability, allowing thrive under adverse conditions. This work reviews effect DSEs subjacent mechanisms will help develop higher tolerance

Language: Английский

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The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Astragalus mongholicus under Cadmium Stress

Agronomy, Journal Year: 2024, Volume and Issue: 14(8), P. 1801 - 1801

Published: Aug. 15, 2024

Dark septate endophytes (DSE) commonly inhabit the root systems of plants that flourish in heavy metal-contaminated soils. Nevertheless, there is limited understanding regarding overall response medicinal to DSE under metal stress. The present study utilized a non-sterile pot experiment evaluate beneficial impacts (Paraphoma chlamydocopiosa, Paraboeremia selaginellae, and Paraphoma radicina) inoculation on Astragalus mongholicus cadmium (Cd) At 0 mg Cd/kg soil, with led significant increase total biomass host plant by up 34.0%. Although decreased at 5 soil 10 increased 72.3% after inoculation. height, branch number, analyzer development (SPAD) value, were not affected Cd concentration P. chlamydocopiosa selaginellae. emerged as most prominent explanatory variable (44.2%) for growth soil. Inoculation length 47.9% 74.1%, selaginellae indole-3-acetic acid (IAA) content 103.6% 105.8%, respectively, Additionally, radicina was preferred calycosin-7-O-glucoside, while formononetin. enhanced accumulation shoot, whereas exhibited an inhibitory effect system. translocation factor, which exceeded one. interaction between factors significantly contributed growth. improved organic carbon reduced available nitrogen content, regardless phosphorus Importantly, promote performance calycosin-7-O-glucoside plant, roots These data comprehension ecological function soils demonstrated potential utility strains cultivating plants.

Language: Английский

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Low-severity wildfire prevents catastrophic impacts on fungal communities and soil carbon stability in a fire-affected Douglas-fir ecosystem

Geoderma, Journal Year: 2025, Volume and Issue: 454, P. 117189 - 117189

Published: Jan. 27, 2025

Language: Английский

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Needle in a Haystack: Culturing Plant‐Beneficial Helotiales Lineages From Plant Roots

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(4)

Published: April 1, 2025

ABSTRACT Root‐associated Helotiales fungi are increasingly recognised as beneficial fungal partners promoting plant growth under nutrient‐limited conditions, particularly, in non‐mycorrhizal hosts lacking the ancestral arbuscular mycorrhizal symbiosis. However, ecology of these is still cryptic relatively few lineages have been successfully cultivated from roots for further study. Here, we attempted mass isolation root endophytic to evaluate recovery known plant‐beneficial using a tailored culture‐based approach. We sampled six wild species Brassicaceae, Caryophyllaceae, and Cyperaceae, growing alpine soils. isolated 602 endophytes compared this culturable diversity with one observed via ITS2 metabarcoding. Metabarcoding revealed that taxa dominated communities, 43% detected also represented our collection. Accordingly, most collection (53%) were Helotiales. These isolates, some P solubilisation potential, belonged primarily three Helotialean clades phylogenetically related growth‐promoting or mycorrhizal‐like strains. This analysis highlights plants harbour diverse root‐endophytic Helotiales, isolates obtained promising resource explore mechanisms ecological traits fungi.

Language: Английский

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Plant development influences dynamic shifts in the root compartment microbiomes of wild and domesticated finger millet cultivars

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 30, 2025

Abstract Background Plant-microbe interactions in the rhizosphere and endosphere are crucial for maintaining plant health ecosystem dynamics. These shaped by several factors, including plant’s developmental stage, domestication, specific root compartments. Different cultivars influence unique microbial communities secreting exudates that either support beneficial symbionts or inhibit pathogens. This study examined community structures of wild-type finger millet five domesticated at two stages. Results Our results revealed compartment, domestication significantly root-associated microbiomes. Interestingly, only about 8% core microbiota was consistently shared between soil plants, indicating 92% shifted dynamically depending on type stage. Pseudomonadota , Actinomycedota Bacteroidota were dominant bacterial phyla, while Ascomycota Basidiomycota primary fungal phyla across all samples, displaying distinct abundance patterns. Notably, an increase correlated with a reduction . The most significant shifts composition occurred during flowering primarily driven genus Pseudomonas findings demonstrate stages recruitment taxa to meet needs, particularly various selective highlights active role plants shaping their microbiomes, providing insights into potential manipulating these enhance crop productivity sustainably. Conclusion indicate both host stage assembly structure microbiome. Plant compartments can selectively recruit from associated particular compartment involved. deterministic selection pressures exerted growth development greatly affect communities. has important implications developing sustainable farming practices, reducing reliance chemical fertilizers pesticides, enhancing future productivity.

Language: Английский

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A Dark Septate Endophyte Improves Cadmium Tolerance of Maize by Modifying Root Morphology and Promoting Cadmium Binding to the Cell Wall and Phosphate

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(5), P. 531 - 531

Published: April 29, 2023

Dark septate endophytes (DSEs) can improve the performance of host plants grown in heavy metal-polluted soils, but mechanism is still unclear. A sand culture experiment was performed to investigate effects a DSE strain (Exophiala pisciphila) on maize growth, root morphology, and cadmium (Cd) uptake under Cd stress at different concentrations (0, 5, 10, 20 mg·kg−1). The results indicated that significantly improved tolerance maize, causing increases biomass, plant height, morphology (length, tips, branch, crossing number); enhancing retention roots with decrease transfer coefficient plants; increasing proportion cell wall by 16.0–25.6%. In addition, changed chemical forms roots, resulting decreases proportions pectates protein-integrated 15.6–32.4%, an increase insoluble phosphate 33.3–83.3%. correlation analysis revealed positive relationship between wall. Therefore, both modifying promoting binding walls forming lower activity. These this study provide comprehensive evidence for mechanisms which colonization enhances subcellular distribution forms.

Language: Английский

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The contrasting responses of abundant and rare microbial community structures and co-occurrence networks to secondary forest succession in the subalpine region

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: May 12, 2023

Knowledge of variations in abundant and rare soil microbial communities interactions during secondary forest succession is lacking. Soil samples were gathered from different successional stages (grassland, shrubland, forest) to study the responses bacterial fungal communities, driving factors by Illumina sequencing 16S ITS rRNA genes. The results showed that α-diversities (Shannon index) bacteria fungi revealed no significant changes succession, but increased significantly for bacteria. β-diversities changed succession. Network analysis obvious topological properties (nodes, links, average degree) networks In contrast, these higher than those grassland indicating are more responsive microorganisms. Additionally, greater network complexity due their numbers nodes links. keystone species differed between consisted 1 48 taxa networks, respectively. TP was most important influencing factor communities. Successional plant richness had influences on C:P, SM N:P mainly related properties. Our indicates respond differently

Language: Английский

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Morphogenesis and metabolomics reveal the compatible relationship among Suillus bovinus , Phialocephala fortinii , and their co-host, Pinus massoniana

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(5)

Published: Sept. 7, 2023

ABSTRACT Ectomycorrhizal (ECM) fungi and dark septate endophytes (DSEs) can both form a symbiotic relationship with the same host plant. However, interactions that occur among these two types of their co-hosts are largely unknown. Here, we investigated ECM fungus Suillus bovinus , DSE Phialocephala fortinii co-host Pinus massoniana . We used scanning electron microscopy optical to characterize morphogenesis symbionts employed ultra-high-performance liquid chromatography–tandem mass spectrometry technique assess effects fungal inoculation on root metabolome. Under pure culture conditions, no synergistic or antagonistic were observed between Phi. S. Generally, simultaneously colonize P. roots without affecting each other’s processes. locus where has already invaded but not vice versa, which may be due physical barrier effect mantle. Both significantly promote growth they have N K uptake. Metabolite accumulation patterns in inoculated and/or greatly altered, especially respect organic acids, flavonoids, lipids, phenolic acids. enhanced flavonoid biosynthesis, whereas dual-inoculation treatments mainly induced phenylpropanoid biosynthesis. These findings reveal compatible relationships suggest theoretical basis for co-application when cultivating seedlings. IMPORTANCE The prevalence ectomycorrhizal wide spectrum tree species is well recognized. In this study, co-host, appears superior microniche competition, different metabolite patterns. This first study revealing morphological metabolic mechanisms contribute fungi, DSEs, co-host.

Language: Английский

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