Comparative Genomics and Transcriptomics Analyses Reveal Divergent Plant Biomass-Degrading Strategies in Fungi

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(8), P. 860 - 860

Published: Aug. 18, 2023

Plant biomass is one of the most abundant renewable carbon sources, which holds great potential for replacing current fossil-based production fuels and chemicals. In nature, fungi can efficiently degrade plant polysaccharides by secreting a broad range carbohydrate-active enzymes (CAZymes), such as cellulases, hemicellulases, pectinases. Due to crucial role biomass-degrading (PBD) CAZymes in fungal growth related biotechnology applications, investigation their genomic diversity transcriptional dynamics has attracted increasing attention. this project, we systematically compared genome content PBD six taxonomically distant species, Aspergillus niger, nidulans, Penicillium subrubescens, Trichoderma reesei, Phanerochaete chrysosporium, Dichomitus squalens, well transcriptome profiles during on nine monosaccharides. Considerable variation remarkable transcriptomic were identified, implying preferred source these different methods transcription regulation. addition, specific utilization ability inferred from genomics transcriptomics was with corresponding sugars, improve our understanding conversion process. This study enhances polysaccharide-degrading provides new insights into designing enzyme mixtures metabolic engineering industrial applications.

Language: Английский

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The Sordariomycetes: an expanding resource with Big Data for mining in evolutionary genomics and transcriptomics

Frontiers in Fungal Biology, Journal Year: 2023, Volume and Issue: 4

Published: June 30, 2023

Advances in genomics and transcriptomics accompanying the rapid accumulation of omics data have provided new tools that transformed expanded traditional concepts model fungi. Evolutionary flourished with use classical newer fungal models facilitate study diverse topics encompassing biology development. Technological advances also created opportunity to obtain mine large datasets. One such continuously growing dataset is Sordariomycetes, which exhibit a richness species, ecological diversity, economic importance, profound research history on amenable models. Currently, 3,574 species this class been sequenced, comprising nearly one-third available ascomycete genomes. Among these genomes, multiple representatives genera

Language: Английский

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Genome-wide prediction and transcriptome analysis of sugar transporters in four ascomycete fungi

Bioresource Technology, Journal Year: 2023, Volume and Issue: 391, P. 130006 - 130006

Published: Nov. 10, 2023

The import of plant-derived small sugars by sugar transporters (STs) has received increasing interest due to its important biological role and great industrial potential. STs are targets genetic engineering improve fungal plant biomass conversion. Comparatively analysis the genome-wide prevalence transcriptomics was performed in four filamentous fungi: Aspergillus niger, nidulans, Penicillium subrubescens Trichoderma reesei. Using phylogenetic literature mining, their predicted were divided into ten subfamilies with putative specificities assigned. In addition, transcriptome revealed complex expression profiles among different species, indicating a sophisticated regulation functional diversity STs. Several showed strong co-expression other genes involved utilization, encoding CAZymes catabolic enzymes. This study provides new insights at genomic/transcriptomic level, facilitating biochemical characterization metabolic engineering.

Language: Английский

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Aldo-keto reductases, short chain dehydrogenases/reductases, and zinc-binding dehydrogenases are key players in fungal carbon metabolism

Advances in applied microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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L-Rhamnose Dehydrogenase LraA of Aspergillus niger Shows High Substrate Specificity Matching Its Expression Profile

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 301 - 301

Published: April 10, 2025

L-rhamnose is one of the main monomeric sugars rhamnogalacturonan I and II, which are polysaccharide components pectin. In ascomycete fungus Aspergillus niger it metabolized through non-phosphorylated pathway, first step catalyzed by dehydrogenase (LraA), converting into L-rhamnono-γ-lactone. This enzyme belongs to PFAM PF00106, unlike most other reductases/dehydrogenases involved in fungal sugar catabolism that typically assigned PF00248 PF00107. The enzymes those families have broad substrate specificity some cases been shown be multiple pathways. this study we heterologously produced biochemically characterized A. LraA studied its expression on a set monosaccharides. revealed that, contrast metabolic redox enzymes, highly specific for has no activity substrates tested study. matched profile, only shows significant L-rhamnose. It therefore can concluded evolved with function catabolism, described so far. high also affects biotechnological applications, as may benefit L-rhamnose-based processes, but would less suitable applications involving conversion sugars.

Language: Английский

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Past, present and future of glycolipids from Ustilaginaceae – A review on cellobiose lipids and mannosylerythritol lipids

Journal of Surfactants and Detergents, Journal Year: 2024, Volume and Issue: 27(5), P. 647 - 689

Published: May 6, 2024

Abstract The glycolipids cellobiose lipids (CL) and mannosylerythritol (MEL) are biosurfactants mainly synthesized by microorganisms of the Ustilaginaceae family. They have a large structural diversity, varying in their sugar moieties attached fatty acids, resulting prospectively broad range applications. This literature review provides detailed overview known microbial producers CL MEL, respective metabolic pathways that result different molecular structures. Further, current advances aerobic fermentative synthesis purification methods illustrated. All influencing factors identified to date with regard fermentation highlighted detail: For usually hydrophilic carbon sources used as substrate, whereas hydrophobic metabolized MEL. Nitrogen limitation was described major trigger for glycolipid an acidic pH favored increased production. An applied parameters recent publications (e.g., substrate‐concentrations, feeding approaches) demonstrates future potential MEL production optimization. Foaming during is either combated or exploited foam fractionation first step. processes focus on solvent extractions chromatography laboratory scale need development scale‐up. Finally, environmental hotspots presented optimization potentials highlighted.

Language: Английский

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Whole genome sequence, CAZyme repertoire and sugar metabolic model of the phytopathogenic fungus Kretzschmaria zonata GFP 132

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: May 5, 2025

Language: Английский

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Colony differentiation of saprobic and pathogenic fungi in relation to carbon utilization

Fungal Genetics and Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104001 - 104001

Published: May 1, 2025

Language: Английский

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Genomic Analysis of Aspergillus Section Terrei Reveals a High Potential in Secondary Metabolite Production and Plant Biomass Degradation

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(7), P. 507 - 507

Published: July 22, 2024

Aspergillus terreus has attracted interest due to its application in industrial biotechnology, particularly for the production of itaconic acid and bioactive secondary metabolites. As related species also seem possess a prosperous metabolism, they are high genome mining exploitation. Here, we present draft sequences six from section Terrei one Nidulantes. Whole-genome phylogeny confirmed that is monophyletic. Genome analyses identified between 70 108 key metabolism genes each genomes Terrei, highest rate found genus so far. The respective enzymes fall into 167 distinct families with most them corresponding potentially unique compounds or compound families. Moreover, 53% were only single species, which supports suitability further mining. Intriguingly, this analysis, combined heterologous gene expression metabolite identification, suggested use strategy UV protection different other Aspergillus. Section contains complete plant polysaccharide degrading potential an even higher cellulolytic than Aspergilli, possibly facilitating additional applications these biotechnology.

Language: Английский

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Soybean molasses as a new and low-cost substrate for gluconic acid production by Aspergillus niger

Process Biochemistry, Journal Year: 2024, Volume and Issue: 146, P. 241 - 249

Published: July 24, 2024

Soybean molasses (SM), a sugar-rich by-product obtained from manufacturing soy protein concentrate, is an interesting source of fermentable sugars for biotechnological processes. For the first time, SM was used as new and low-cost substrate in Aspergillus niger cultures to produce gluconic acid (GA), carboxylic by oxidation glucose with wide range applications. The effect composition sugar content on GA batch production studied stirred-tank bioreactor using two samples different total concentrations 46 % 52 (w/w) profiles. productivity improved concentration increased, whereas yield unaffected. Agitation rate increase positively affected medium highest concentration. Under these conditions, titer (79 g·L−1) obtained. Oxalic fructose syrup were other added-value compounds found submerged fermentation SM. This work proved potential A. bioconversion compositions into GA, providing novel strategy eco-friendly

Language: Английский

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