Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production in Trichoderma reesei DOI Creative Commons
David Batista Maués, Jhonatan Christian Maraschin, Diego A. Duarte

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(12), P. 1173 - 1173

Published: Dec. 7, 2023

The fungus Trichoderma reesei is an essential producer of enzymes that degrade lignocellulosic biomass to produce value-added bioproducts. cellulolytic system T. controlled by several transcription factors (TFs) efficiently regulate the production these enzymes. Recently, a new TF named Azf1 was identified as positive regulator cellulase expression. Here, we investigated novel regulatory functions its overexpression. In mutant strain OEazf1, overexpression azf1 achieved under both repression and induction conditions. Although more abundant in transcript protein, this did not activate gene presence repressor glucose, suggesting may be subject posttranslational regulation. cellulose, expression swo, encoding accessory protein swollenin, β-glucosidases cel1a, cel1b, cel3b, cel3g increases early stages cultivation. increased hydrolysis rate cellobiose sophorose, which activates carbon catabolite (CCR) causes genes Xyr1 later Moreover, led activity during long-term cultivation cellulose sugarcane bagasse. Our results provide insights into mechanisms regulating are important targets TF. This work contributes better understanding complex reesei. It will contribute development strains with higher

Language: Английский

Fungal strain improvement for efficient cellulase production and lignocellulosic biorefinery: Current status and future prospects DOI
Jie Yang,

Hou-Ru Yue,

Li-Ya Pan

et al.

Bioresource Technology, Journal Year: 2023, Volume and Issue: 385, P. 129449 - 129449

Published: July 4, 2023

Language: Английский

Citations

30
Trichodenoids A and B, Two Skeletally Unprecedented Polyketides from Trichoderma reesei with Cardioprotective Effects against H2O2-Induced Injury DOI
Rui Jiang, Jiayi Guo, Shenglan Yang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Trichoderma reesei, recognized by the FDA as a food-safe strain, plays vital role in food fermentation. Although enzymatic applications of T. reesei are well-established, health benefits its fermentation-derived metabolites yet to be fully explored. Trichodenoids A (1) and B (2), two skeletally unprecedented polyketides, were isolated from endophytic fungus originating plant Gastrodia elata Blume. Their structures elucidated via spectroscopic data, single-crystal X-ray crystallographic analysis, quantum chemical DP4+ ECD calculation. Compounds 1 2 uniquely defined unusual 6/6/5 6/6/5/6 ring systems, respectively, which proposed formed through key Diels-Alder Baeyer-Villiger reactions during biosynthesis. Compound had potential mitigate H2O2-induced oxidative stress H9C2 cells reducing intracellular ROS levels, restoring mitochondrial function, regulating mRNA expression related stress, inflammation, autophagy. These findings highlight compound candidate for natural antioxidants even dietary supplements cardiovascular health.

Language: Английский

Citations

0
Cutting-edge advances in strain and process engineering for boosting cellulase production in Trichoderma reesei DOI
Qingshan Meng, Brett Abraham, Jinguang Hu

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 419, P. 132015 - 132015

Published: Dec. 22, 2024

Language: Английский

Citations

1
Overexpression of a Novel Vacuolar Serine Protease-Encoding Gene (spt1) to Enhance Cellulase Production in Trichoderma Reesei DOI Creative Commons
Yao Cheng,

Ningning Sun,

Weihao Gao

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(2), P. 191 - 191

Published: Feb. 19, 2023

Trichoderma reesei is widely applied as the major industrial fungus for production of cellulases used conversion lignocellulosic biomass to biofuels and other biobased products. The protein secretion pathway vital cellulase secretion, but few reports are related role vacuole in production. Here, we identified a novel vacuolar serine protease gene spt1 investigated ability T. secrete by disrupting, complementing overexpressing gene. Amino acid sequence analysis Spt1 showed that it belongs subtilisin S8 family has conserved catalytic triples (Asp, His, Ser) protease. deletion did not lead decrease extracellular activity, observation mycelia with Spt1–eGFP fusion expression membrane dye FM4-64 staining confirmed was an intracellular located vacuoles reesei. However, significantly reduced spore while complementation recovered these traits those parental strain. When overexpressed using its native promoter introducing multiple copies, improved. Furthermore, strong promoter, Pcdna1, drive overexpression, found enhanced. Specifically, filter paper activity overexpression strain SOD-2 reached 1.36 U/mL, which 1.72 times higher than These findings demonstrated can be powerful target increasing reesei, suggests possible important provides new clues improving strains efficient

Language: Английский

Citations

3
Dual inhibition of vacuolar ATPase subunit H and iron availability induces intrinsic apoptosis in Penicillium digitatum from citrus fruit DOI

Huilin Kang,

Yingying Zhao, Gang Fan

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 221, P. 113327 - 113327

Published: Nov. 23, 2024

Language: Английский

Citations

0
Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production in Trichoderma reesei DOI Creative Commons
David Batista Maués, Jhonatan Christian Maraschin, Diego A. Duarte

et al.

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(12), P. 1173 - 1173

Published: Dec. 7, 2023

The fungus Trichoderma reesei is an essential producer of enzymes that degrade lignocellulosic biomass to produce value-added bioproducts. cellulolytic system T. controlled by several transcription factors (TFs) efficiently regulate the production these enzymes. Recently, a new TF named Azf1 was identified as positive regulator cellulase expression. Here, we investigated novel regulatory functions its overexpression. In mutant strain OEazf1, overexpression azf1 achieved under both repression and induction conditions. Although more abundant in transcript protein, this did not activate gene presence repressor glucose, suggesting may be subject posttranslational regulation. cellulose, expression swo, encoding accessory protein swollenin, β-glucosidases cel1a, cel1b, cel3b, cel3g increases early stages cultivation. increased hydrolysis rate cellobiose sophorose, which activates carbon catabolite (CCR) causes genes Xyr1 later Moreover, led activity during long-term cultivation cellulose sugarcane bagasse. Our results provide insights into mechanisms regulating are important targets TF. This work contributes better understanding complex reesei. It will contribute development strains with higher

Language: Английский

Citations

0