Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 22, 2025
Abstract
Rhizosphere
and
root
associated
fungi,
commonly
known
as
plant
growth
promoting
fungi
(PGPF),
facilitate
the
productivity
of
Cucumis
sativus
L.
through
formation
mutualistic
relationships
with
roots.
These
enhance
nutrient
uptake,
promote
development
bolster
stress
tolerance
via
secretion
regulating
hormones.
Despite
numerous
PGP
fungal
species
have
been
isolated
from
rhizosphere
C.
sativus,
their
specific
roles
in
modulating
various
parameters
remain
insufficiently
defined.
In
present
study,
effect
seven
were
evaluated
such
Penicillium
chrysogenum,
Paecilomyces
variotii,
Talaromyces
purpureogenus,
brunneolus,
Aspergillus
flavus,
Trichoderma
viride,
atroviride
on
sativus.
Our
results
revealed
that
all
positively
influenced
as,
length,
shoot
fresh
dry
biomass,
leaf
area,
moisture
content,
flower
number,
area
ratio,
weight
ratio
chlorophyll
albeit
varying
degrees
efficiency.
Notably,
exhibited
a
more
pronounced
impact
compared
to
other
control.
suggest
these
may
contribute
sustainable
cultivation
practices
by
enhancing
crop
yield
reducing
dependency
chemical
fertilizers
pesticides.
Plant Stress,
Journal Year:
2024,
Volume and Issue:
11, P. 100426 - 100426
Published: March 1, 2024
Soil
salinity
is
one
of
the
major
environmental
stresses
that
significantly
hampers
crop
yield
worldwide.
This
study
assessed
effectiveness
rhizospheric
fungus,
Aspergillus
sojae
JUSPF-101,
in
enhancing
tolerance
finger
millet
seedlings
to
NaCl
stress.
The
research
also
examined
role
JUSPF-101
producing
phytohormones
and
volatile
organic
compounds.
impact
A.
was
evaluated
on
both
primed
unprimed
(using
Petri
dish
pipette
tip
bioassay
methods)
under
stress
conditions.
A
conducted
a
greenhouse
confirm
growth
characteristics
mineral
composition
seedlings,
with
without
inoculation
JUSPF-101.
These
were
exposed
varying
concentrations
(0
400
mM).
current
demonstrated
could
withstand
high
(up
1200
mM)
temperatures
(45°C).
Using
LC-MS/MS,
fifteen
identified
grown
0
mM.
include
IAA,
SA,
GA4,
epibrasinolide,
trans
isomer
zeatin,
cis-jasmone.
GC-MS
analysis
detected
seventy-seven
VOCs,
compound
being
1-(4-Amino-furazan-3-yl)-5-propyl-1H-[1,2,3]triazole-4-carboxylic
acid
ethyl
ester
(8.7
%).
Moreover,
not
treated
displayed
significant
decrease
RWC
biochemical
parameters
such
as
proline,
phenols,
flavonoids
(p
<
0.05),
while
there
an
increase
MDA
H2O2
content.
However,
using
effectively
alleviated
caused
by
led
improvements
RWC,
growth,
parameters.
application
mM
treatment,
increased
morphological
(shoot
root
length)
proline
(1.67-fold),
phenols
(2.15-fold),
(2.04-fold)
(1.2-fold),
(1.25-fold)
decreased
compared
In
environment,
showed
improved
lower
Na+/K+
Na+/Ca2+
ratios.
findings
suggest
applying
(JUSPF-101)
be
effective
strategy
for
reducing
millet,
contributing
sustainable
food
security.
Journal of Pest Science,
Journal Year:
2024,
Volume and Issue:
97(4), P. 1899 - 1912
Published: Feb. 4, 2024
Abstract
This
study
delves
into
the
compatible
use
of
a
parasitoid
with
multifunctional
endophytic
Entomopathogenic
Ascomycetes
(EA)
in
IPM
under
greenhouse
conditions.
The
Hyposoter
didymator
was
evaluated
against
Spodoptera
littoralis
multitrophic
system
melon
plants
that
were
endophytically
colonized
by
one
three
EA
strains
(
Metarhizium
brunneum
[one]
or
Beauveria
bassiana
[two]).
In
first
scenario,
inoculated
different
methods,
and
after
infestation
noctuid
larvae,
released
at
1:20
ratio.
Microbiological
molecular
techniques
allowed
identification
progressive
colonization
throughout
whole
plant
life
cycle,
for
B.
,
approximately
20%
seeds
from
new
fruits
colonized.
shown
to
be
all
application
total
mortality
rates
ranging
11.1
77.8%.
Significant
lethal
sublethal
effects,
decrease
pupal
weight
pupae
showing
abnormalities
an
extension
immature
developmental
times
observed
strain–application
method
combinations.
Additionally,
fungal
treatments
improved
crop
growth,
as
revealed
significant
gains
weight.
second
scenario
(by
inoculating
fungi
only
leaf
spraying),
1:10
ratio,
which
remote
effect
inoculation
point
confirmed
compatibility
parasitoid-EA-based
strategy.
These
findings
underscore
S.
control
can
additionally
exploit
their
multifunctionality
sustainable
production.
Biology,
Journal Year:
2024,
Volume and Issue:
13(3), P. 190 - 190
Published: March 15, 2024
Salinity
severely
affects
the
health
and
productivity
of
plants,
with
root-associated
microbes,
including
fungi,
potentially
playing
a
crucial
role
in
mitigating
this
effect
promoting
plant
health.
This
study
employed
metagenomics
to
investigate
differences
structures
epiphyte
mycobiomes
rhizospheres
seedlings
two
distinct
date
palm
cultivars
contrasting
salinity
tolerances,
susceptible
cultivar,
‘Zabad’,
tolerant
‘Umsila’.
Next-generation
sequencing
(NGS)
internal
transcribed
spacer
(ITS)
rRNA
was
utilized
as
DNA
barcoding
tool.
The
12
mycobiome
libraries
yielded
905,198
raw
sequences
268,829
high-quality
reads
that
coded
for
135
unique
annotatable
operational
taxonomic
units
(OTUs).
An
OTU
analysis
revealed
rhizofungal
community
between
treatments
regardless
genotype,
non-metric
dimensional
scaling
(N-MDS)
analyses
demonstrated
separations
under
saline
stress.
However,
these
were
not
detected
control
environmental
conditions,
i.e.,
no
salinity.
rhizospheric
fungal
included
four
phyla
(Ascomycota,
Basidiomycota,
Chytridiomycota,
Mucoromycota),
abundances
Aspergillus,
Clonostachys,
Fusarium
genera
response
salinity,
genotype.
Differential
pairwise
comparisons
showed
falciforme-solani
Aspergillus
sydowii-versicolor
increased
abundance
providing
potential
future
vitro
isolation
guidelines
growth-promoting
fungi.
highlights
intricate
dynamics
rhizosphere
microbial
communities
palms
their
responses
salt
Additionally,
we
found
support
hypothesis
indigenous
epiphytic
are
significantly
involved
tolerance
palms.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: June 17, 2024
Abstract
Rice
straw
breakdown
is
sluggish,
which
makes
agricultural
waste
management
difficult,
however
pretreatment
procedures
and
cellulolytic
fungi
can
address
this
issue.
Through
ITS
sequencing,
Chaetomium
globosum
C1,
Aspergillus
sp.
F2,
Ascomycota
SM2
were
identified
from
diverse
sources.
exhibited
the
highest
carboxymethyl
cellulase
(CMCase)
activity
(0.86
IU/mL)
filter-paper
(FPase)
(1.054
FPU/mL),
while
F2
showed
CMCase
(0.185
after
various
pretreatments
of
rice
straw.
These
thrived
across
a
wide
pH
range,
with
4
to
9,
C1
thriving
in
alkaline
conditions
(pH
9).
FTIR
spectroscopy
revealed
significant
structural
changes
enzymatic
hydrolysis
solid-state
fermentation,
indicating
lignin,
cellulose,
hemicellulose
degradation.
Soil
amendments
pretreated
straw,
cow
manure,
biochar,
these
increased
root
growth
soil
nutrient
availability,
even
under
severe
salt
stress
(up
9.3
dS/m).
The
study
emphasizes
need
for
better
understanding
degradation
capabilities
proposes
that
using
fungus
into
could
mitigate
salt-related
difficulties
improve
availability
salty
soils.
BMC Genomic Data,
Journal Year:
2025,
Volume and Issue:
26(1)
Published: Jan. 24, 2025
Clonostachys,
a
genus
with
rich
morphological
and
ecological
diversity
in
Bionectriaceae,
has
wide
distribution
among
diverse
habitats.
Several
studies
have
reported
Clonostachys
fungi
as
effective
biological
agents
against
multiple
fungal
plant
pathogens.
To
clarify
the
biocontrol
mechanisms
of
fungi,
this
study
was
undertaken
to
sequence
assemble
genomes
two
C.
chloroleuca
one
rhizophaga.
Here,
we
performed
genomic
sequencing
three
strains
collected
from
China
General
Microbiological
Culture
Collection
Center
(CGMCC)
using
Illumina
HiSeq
2500
technology.
Whole
genome
analysis
indicated
that
their
consist
58,484,224
bp
GC
content
48.58%,
58,114,960
47.74%
58,450,453
respectively.
BUSCO
assembly
completeness
these
at
least
98%.
In
summary,
datasets
provide
valuable
resource
for
ongoing
include
further
exploration
function,
marker
development,
enhanced
control
ability
population
diversity.
Journal of Fungi,
Journal Year:
2025,
Volume and Issue:
11(2), P. 153 - 153
Published: Feb. 17, 2025
Penicillium
griseofulvum
CF3
is
a
fungus
isolated
from
healthy
strawberry
soil,
with
the
potential
to
promote
growth
of
plants
and
enhance
their
resistance
diseases.
However,
genome
sequence
P.
remains
unclear.
Therefore,
we
performed
whole-genome
CCS
sequencing
using
PacBio
Sequel
II
platform.
The
assembled
comprised
104
contigs,
total
length
37,564,657
bp,
encoding
13,252
protein-coding
genes.
Comprehensive
functional
annotation
was
various
BLAST
databases,
including
non-redundant
(Nr)
protein
database,
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
EuKaryotic
Orthologous
Groups
(KOG),
Carbohydrate-Active
enZymes
(CAZy)
identify
predict
genes,
tRNAs,
rRNAs.
Antibiotics
Secondary
Metabolites
Analysis
Shell
(Antismash)
analysis
identified
50
biosynthetic
gene
clusters
involved
in
secondary
metabolite
production
within
genome.
helps
us
understand
its
mechanisms
promoting
plant
enhancing
disease
resistance,
paving
way
for
application
strain
sustainable
crop
production.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(3), P. 543 - 543
Published: Feb. 27, 2025
Drought,
exacerbated
by
global
warming,
poses
a
significant
threat
to
crop
growth
and
productivity.
This
study
identified
strain
of
Trichoderma
harzianum
from
the
rhizosphere
healthy
Nicotiana
tabacum
L.
plants
evaluated
its
role
in
enhancing
drought
tolerance.
The
isolated
effectively
colonized
plant
roots
promoted
N.
To
investigate
potential,
T.
was
inoculated
into
under
varying
conditions,
impact
on
growth,
physiological
responses,
resilience
assessed.
Comprehensive
analyses
agronomic
traits,
parameters,
enzyme
activities,
photosynthetic
performance,
osmoprotectant
levels,
membrane
lipid
peroxidation
revealed
that
inoculation
(light
with
harzianum,
moderate
severe
treatments)
systematically
improved
development
resistance.
These
findings
provide
valuable
insights
lay
foundation
for
developing
innovative
biofertilizers
enhance
tolerance
sustainability.
