Redundant and Distinct Roles of Two 14-3-3 Proteins in Fusarium sacchari, Pathogen of Sugarcane Pokkah Boeng Disease DOI Creative Commons
Yuejia Chen, Ziting Yao,

Lixian Zhao

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(4), P. 257 - 257

Published: March 28, 2024

Fusarium sacchari, a key pathogen of sugarcane, is responsible for the Pokkah boeng disease (PBD) in China. The 14-3-3 proteins have been implicated critical developmental processes, including dimorphic transition, signal transduction, and carbon metabolism various phytopathogenic fungi. However, their roles are poorly understood F. sacchari. This study focused on characterization two protein-encoding genes, FsBmh1 FsBmh2, within Both genes were found to be expressed during vegetative growth stage, yet was repressed at sporulation stage vitro. To elucidate functions these deletion mutants ΔFsBmh1 ΔFsBmh2 generated. exhibited more pronounced phenotypic defects, such as impaired hyphal branching, septation, conidiation, spore germination, colony growth, compared ΔFsBmh1. Notably, both knockout showed reduction virulence, with transcriptome analysis revealing changes associated observed phenotypes. further investigate functional interplay between we constructed analyzed combined silencing (ΔFsBmh/siFsBmh) well overexpression (O-FsBmh). combinations ΔFsBmh1/siFsBmh2 or ΔFsBmh2/siFsBmh1 displayed severe phenotypes than those single allele deletions, suggesting redundancy proteins. Yeast two-hybrid (Y2H) assays identified 20 pivotal primary diverse biological functions, 12 which interacted FsBmh2. Three specifically FsBmh1, while five exclusively In summary, this research provides novel insights into FsBmh2 sacchari highlights potential targets PBD management through modulation FsBmh functions.

Language: Английский

Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora DOI Creative Commons
Qianqian Liu, Kexin Jiang,

Shipeng Duan

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.

Language: Английский

Citations

6
Mitochondrial Porin Is Required for Versatile Biocontrol Trait-Involved Biological Processes in a Filamentous Insect Pathogenic Fungus DOI
Haonan Feng, Ning Peng, Xin Zhao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(3), P. 2088 - 2102

Published: Jan. 11, 2025

The mitochondrial voltage-dependent anion channel (VDAC) is the major in outer membrane for metabolites and ions. VDACs also regulate a variety of biological processes, which vary number VDAC isoforms across different eukaryotes. However, little known about VDAC-mediated biocontrol traits fungi. Here, only isoform (BbOmm1) filamentous insect pathogenic fungus Beauveria bassiana was characterized, crucial maintenance homeostasis function important traits. Besides serious impairment fungal growth, conidiation, germination, inactivation BbOmm1 led to increased sensitivity/tolerance oxidative osmotic stresses production oosporein other secondary metabolites, corresponded damage, downregulation lipid cell wall homeostasis-involved genes, upregulation detoxification genes biosynthesis gene clusters those metabolites. These results enrich our understanding pathogens.

Language: Английский

Citations

0
The Cryptochrome CryA Regulates Lipid Droplet Accumulation, Conidiation, and Trap Formation via Responses to Light in Arthrobotrys oligospora DOI Creative Commons

Yanmei Shen,

Xuewei Yang, Meichen Zhu

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(9), P. 626 - 626

Published: Sept. 1, 2024

Light is a key environmental factor affecting conidiation in filamentous fungi. The cryptochrome/photolyase CryA, blue-light receptor, involved fungal development. In the present study, homologous CryA (AoCryA) was identified from widely occurring nematode-trapping (NT) fungus Arthrobotrys oligospora, and its roles mycelial growth development of A. oligospora were characterized using gene knockout, phenotypic comparison, staining technique, metabolome analysis. inactivation AocryA caused substantial decrease spore yields dark conditions but did not affect wild-type (WT) ∆AocryA mutant strains light conditions. Corresponding to production, transcription sporulation-related genes also significantly downregulated Contrarily, mutants showed increase trap formation conditions, while production abilities WT decreased addition, lipid droplet accumulation increased an tolerance sorbitol, contributed synthesis carotenoids. Finally, AoCryA found secondary metabolic processes. These results reveal, for first time, function cryptochrome NT

Language: Английский

Citations

0
Involvement of AoMdr1 in the Regulation of the Fluconazole Resistance, Mycelial Fusion, Conidiation, and Trap Formation of Arthrobotrys oligospora DOI Creative Commons
Yankun Liu, Xuewei Yang, Meichen Zhu

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1612 - 1612

Published: June 19, 2023

Multidrug resistance (Mdr) proteins are critical for maintenance of drug in fungi. Mdr1 has been extensively studied Candida albicans; its role other fungi is largely unknown. In this study, we identified a homologous protein Mdr (AoMdr1) the nematode-trapping (NT) fungus Arthrobotrys oligospora. It was found that deletion Aomdr1 resulted significant reduction number hyphal septa and nuclei as well increased sensitivity to fluconazole hyperosmotic stress SDS. The also led remarkable increase numbers traps mycelial loops traps. Notably, AoMdr1 able regulate fusion under low-nutrient conditions, but not nutrient-rich conditions. involved secondary metabolism, caused an arthrobotrisins (specific compounds produced by NT fungi). These results suggest plays crucial resistance, fusion, conidiation, trap formation, metabolism A. Our study contributes understanding growth development

Language: Английский

Citations

1
Redundant and Distinct Roles of Two 14-3-3 Proteins in Fusarium sacchari, Pathogen of Sugarcane Pokkah Boeng Disease DOI Creative Commons
Yuejia Chen, Ziting Yao,

Lixian Zhao

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(4), P. 257 - 257

Published: March 28, 2024

Fusarium sacchari, a key pathogen of sugarcane, is responsible for the Pokkah boeng disease (PBD) in China. The 14-3-3 proteins have been implicated critical developmental processes, including dimorphic transition, signal transduction, and carbon metabolism various phytopathogenic fungi. However, their roles are poorly understood F. sacchari. This study focused on characterization two protein-encoding genes, FsBmh1 FsBmh2, within Both genes were found to be expressed during vegetative growth stage, yet was repressed at sporulation stage vitro. To elucidate functions these deletion mutants ΔFsBmh1 ΔFsBmh2 generated. exhibited more pronounced phenotypic defects, such as impaired hyphal branching, septation, conidiation, spore germination, colony growth, compared ΔFsBmh1. Notably, both knockout showed reduction virulence, with transcriptome analysis revealing changes associated observed phenotypes. further investigate functional interplay between we constructed analyzed combined silencing (ΔFsBmh/siFsBmh) well overexpression (O-FsBmh). combinations ΔFsBmh1/siFsBmh2 or ΔFsBmh2/siFsBmh1 displayed severe phenotypes than those single allele deletions, suggesting redundancy proteins. Yeast two-hybrid (Y2H) assays identified 20 pivotal primary diverse biological functions, 12 which interacted FsBmh2. Three specifically FsBmh1, while five exclusively In summary, this research provides novel insights into FsBmh2 sacchari highlights potential targets PBD management through modulation FsBmh functions.

Language: Английский

Citations

0