Biofungicides Based on Plant Extracts: On the Road to Organic Farming

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6879 - 6879

Published: June 22, 2024

Phytopathogenic fungi are responsible for diseases in commercially important crops and cause major supply problems the global food chain. Plants were able to protect themselves from disease before humans played an active role protecting plants. They known synthesize a variety of secondary metabolites (SMs), such as terpenes, alkaloids, phenolic compounds, which can be extracted using conventional unconventional techniques formulate biofungicides; plant extracts have antifungal activity various mechanisms action against these organisms. In addition, they considered non-phytotoxic potentially effective control. sustainable economically viable alternative use agriculture, is why biofungicides increasingly recognized attractive option solve caused by synthetic fungicides. Currently, organic farming continues grow, highlighting importance developing environmentally friendly alternatives crop production. This review provides compilation literature on biosynthesis, phytopathogens, extraction formulation biofungicides, biological phytopathogenic fungi, regulation, advantages, disadvantages overview current agriculture.

Language: Английский

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Genome Sequencing and Characterization of Bacillus velezensis N23 as Biocontrol Agent against Plant Pathogens

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 294 - 294

Published: Jan. 30, 2024

The overuse of chemical fungicides against fungal pathogens adversely affects soil and plant health, resulting in environmental problems food safety. Therefore, biocontrol is considered as an environmentally friendly cost-effective green technique protection agricultural production. We obtained a bacterial strain N23 from contaminated plate which showed significant inhibition to anthracnose. was identified Bacillus velezensis based on 16S rRNA gene, gyrA whole-genome sequence. bacterium able suppress the mycelial growth numerous pathogenic fungi solid media. Tomato seeds treated with significantly higher germination levels than untreated ones. Moreover, effectively reduced lesion area pepper anthracnose disease planta. gene clusters responsible for antifungal metabolites (fengycin, surfactin, iturin) were genome sequence mining PCR. Furthermore, methanol extracts culture caused Colletotrichum sp. Botrytis cinerea. These findings suggested that B. could be potential agent production source antimicrobial compounds further exploitation.

Language: Английский

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Exploitation of Post-Ripening Treatment for Improving Cold Tolerance and Storage Period of Jin Huang Mango

Horticulturae, Journal Year: 2024, Volume and Issue: 10(1), P. 103 - 103

Published: Jan. 20, 2024

The limited cold tolerance of the Jin Huang mango represents a significant impediment to its potential for international trade. Therefore, this study evaluated quality index changes green maturity mangoes with different post-ripening treatments and then when stored at storage periods (7, 14, 21, 28) 4 °C followed by 6 days 20 °C. This showed that treated 500 ppm ethylene were slow ripen during storage, which could be sustainable even under storage. In addition, control (CK) group failed mature or unevenly after Moreover, T3 (ethylene ripening 1 day day) minimized occurrence CI compared CK while contributing 30% decrease in anthracnose incidence firmness titratable acid (TA), total soluble solids (TSS) notably increased, yet ascorbic content was lower. Hence, treatment conditions using helped extend shelf-life °C, stocking minimizing anthracnose, thereby maintaining certain quality.

Language: Английский

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Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 8, 2025

Endophytic fungi associated with selected aquatic plants, Eichhornia crassipes, Nymphaea nouchali, Salvinia minima and S. molesta were evaluated. Ectophoma salviniae sp. nov. Neottiosporina mihintaleensis are introduced as novel taxa from spp. Sri Lanka. Chaetomella raphigera is reported a new geographical record, Colletotrichum siamense C. truncatum host records in while Phyllosticta capitalensis has been identified on the same (Nymphaea nouchali) North-Central Province of Identification was based morphological characteristics multi-locus phylogenetic analyses using ITS, LSU, SSU, ACT, CHS-1, GAPDH, tub2, rpb2, tef1-α molecular markers. The analysed for extracellular enzymatic properties. According to qualitative analysis, exhibited highest amylase production, cellulase enzyme laccase production. results demonstrate fungal diversity this region their potentials, providing valuable insights future biotechnological approaches.

Language: Английский

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Optimization, characterization and biosafety of oregano, rosemary and mint oil mixture against Penicillium digitatum in citrus using L-optimal mixture design

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 27, 2025

Abstract The increasing demand for natural alternatives to synthetic fungicides has prompted research into products like essential oils postharvest disease management. This study investigated the antifungal, antioxidant, cytotoxic, and genotoxic potential of oil mixtures derived from oregano, rosemary, mint against Penicillium digitatum , predominant fungal pathogen causing green mold in orange fruits. P. NPAGRASU 2024 was isolated identified as most abundant species (60.2%) infected oranges. It deposited GenBank with gene accession number PP930644 MIRCEN culture collection EMCC 358874. antimicrobial activity individual evaluated, oregano exhibiting highest antifungal (inhibition zone diameter 4.2 cm) . L-optimal mixture design response surface methodology (RSM) optimization revealed a highly effective (Run 8) comprising 46.26% 53.74% 99.65% actual growth reduction. demonstrated potent antioxidant activity, reaching approximately 75% DPPH radical scavenging at 3.125 mg/mL. Cytotoxicity assessment using MTT assay showed morphological changes reduced cell viability liver cells treated PEOs 300 µg/mL. However, optimized did not induce significant chromosomal aberrations compared control, suggesting minimal effects. In vivo, evaluation on oranges 60% inhibition by 1% (v/v) 7 days. Histological analysis indicated low toxicity tested concentration (1% mixture). GC-MS major compounds cavarcol, caryophyllene, eucalyptol, phenols levomenthol oil, contributing its bioactivities. demonstrates eco-friendly control.

Language: Английский

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Synthesis, Characterization, and Evaluation of the Antifungal Properties of 3-Indolyl-3-Hydroxy Oxindole Derivatives Against Plant Pathogenic Fungi

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1079 - 1079

Published: Feb. 26, 2025

To discover novel fungicides with good inhibitory effects on plant fungal diseases, twenty-five 3-indolyl-3-hydroxy oxindole derivatives (3a-3y) were synthesized. These newly characterized by NMR and HRMS. Their antifungal activities against five pathogenic fungi assessed in vitro. Most of the compounds exhibited moderate to excellent fungi. Notably, 3t, 3u, 3v, 3w displayed remarkable broad-spectrum comparable or superior those carvacrol (CA) phenazine-1-carboxylic acid (PCA). Among them, compound 3u most activity Rhizoctonia solani Kühn (R. solani), an EC50 3.44 mg/L, which was CA (7.38 mg/L) PCA (11.62 mg/L). Preliminary structure-activity relationship (SAR) results indicated that introduction I, Cl, Br substituents at position 5 3-hydroxy-2-oxindole indole rings is crucial for exhibit activity. The vivo assay showed has curative R. solani. current suggest these are capable serving as promising lead compounds.

Language: Английский

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Antifungal spectrum of chitosan films added with lactic acid bacteria cell-free supernatants

Food and Humanity, Journal Year: 2025, Volume and Issue: unknown, P. 100552 - 100552

Published: March 1, 2025

Language: Английский

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Characterization and pathogenicity of Colletotrichum species causing anthracnose on pitaya (Hylocereus spp.) in Brazil

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102657 - 102657

Published: March 1, 2025

Language: Английский

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Post-harvest Physiology of Vegetable Crops and Its Regulation

Published: Jan. 1, 2025

Language: Английский

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Complete genome analysis and antimicrobial mechanism of Bacillus velezensis GX0002980 reveals its biocontrol potential against mango anthracnose disease

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

ABSTRACT Post-harvest anthracnose significantly affects the yield and quality of mangoes. In this study, an antagonist bacterium, GX0002980, with strong inhibitory effect against Colletotrichum gloesporioides , pathogen mango anthracnose, was isolated from rhizosphere soil plants. Based on morphological characteristics, physiological biochemical tests, 16S rRNA gene gyrB sequencing analysis, strain identified as Bacillus velezensis . Strain GX0002980 exhibits broad-spectrum antibacterial capabilities, its volatile substances sterile fermentation filtrate also demonstrate antagonistic effects C. This can produce antimicrobial that inhibit growth, such amylase, cellulase, protease, pectinase, siderophores, has plant-growth-promoting nitrogen fixation, NH 3 production, phosphate solubilization. Whole-genome results show genome size is 3,907,381 bp a guanine cytosine content 47.44%. The antiSMASH analysis predicts 14 biosynthesis clusters within genome, including surfactin, fengycin, bacilysin, macrolactin H, bacillaene, difficidin, bacillibactin. Liquid chromatography-mass spectrometry revealed active secreted by include butirosin A, more. in vitro control efficiency 52% it effectively suppress occurrence post-harvest diseases mangoes, extending their storage time. conclusion, B. possesses multiple biocontrol mechanisms potential for application biological anthracnose. IMPORTANCE showed causative agent produces variety secondary metabolites properties. metabolite synthesis GX0002980. significant disease fruits.

Language: Английский

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