Light Regulates Secreted Metabolite Production and Antagonistic Activity in Trichoderma DOI Creative Commons
Edgardo Ulises Esquivel‐Naranjo,

Hector Mancilla-Diaz,

Rodolfo Márquez

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 11(1), P. 9 - 9

Published: Dec. 26, 2024

Secondary metabolism is one of the main mechanisms Trichoderma uses to explore and colonize new niches, 6-pentyl-α-pyrone (6-PP) an important secondary metabolite in this process. This work focused on standardizing a method investigate production 6-PP. Ethanol ethyl acetate were both effective solvents for quantifying 6-PP solution had limited solubility potato–dextrose–broth media. The extraction using provided rapid efficient process recover metabolite. was readily produced during development atroviride growing dark, but light suppressed its production. purified, spectrum by nuclear magnetic resonance mass spectroscopy identical that commercial Light also induced or other unidentified metabolites several species Trichoderma. antagonistic activity T. influenced light, as suppression plant pathogens greater dark. secreted potato–dextrose–agar differentially regulated indicating with against pathogens. has influence Trichoderma, trait key relevance selecting strains protection.

Language: Английский

Uncovering the multifaceted properties of 6-pentyl-alpha-pyrone for control of plant pathogens DOI Creative Commons
Artemio Mendoza‐Mendoza, Edgardo Ulises Esquivel‐Naranjo, Sereyboth Soth

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 18, 2024

Some volatile organic compounds (VOCs) produced by microorganisms have the ability to inhibit growth and development of plant pathogens, induce activation defenses, promote growth. Among them, 6-pentyl-alpha-pyrone (6-PP), a ketone Trichoderma fungi, has emerged as focal point interest. 6-PP been isolated characterized from thirteen species is main VOC produced, often accounting for >50% total VOCs emitted. This review examines abiotic biotic interactions regulating production , known effects on pathogens through direct indirect mechanisms including induced systemic resistance. While there are many reports activity against vast majority laboratory studies involving only pathogen, rather than glasshouse or field host in system. Biopesticides based may well provide an eco-friendly, sustainable management tool future agricultural production. However, before this can happen, challenges demonstrating disease control efficacy field, developing efficient delivery systems, determining cost-effective application rates must be overcome 6-PP’s potential pathogen turned into reality.

Language: Английский

Citations

4

Light Spectrum Variations: Implications for Growth and Bioactive Compound Synthesis in Trigonella persica DOI
Ehsan Bakhshy, Fatemeh Zarinkamar, Mehrdad Nazari

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106117 - 106117

Published: Feb. 1, 2025

Language: Английский

Citations

0

Linking a polyketide synthase gene cluster to 6-pentyl-alpha-pyrone, a Trichoderma metabolite with diverse bioactivities DOI Creative Commons
Daniel Flatschacher,

Alexander Eschlböck,

Siebe Pierson

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 21, 2025

Abstract Background Members of the fungal genus Trichoderma are well-known for their mycoparasitic and plant protecting activities, rendering them important biocontrol agents. One most significant specialized metabolites (SMs) produced by various species is unsaturated lactone 6-pentyl-alpha-pyrone (6-PP). Although first identified more than 50 years ago having pronounced antifungal growth-promoting properties, biosynthetic pathway 6-PP still remains unresolved. Results Here, we demonstrate that biosynthesized via polyketide biosynthesis pathway. We Pks1, an iterative type I synthase, as crucial its in atroviride , a recognized prominent production abilities. Phylogenetic comparative genomic analyses revealed pks1 gene part cluster conserved those known to produce 6-PP. Deletion caused complete loss T. reduction activity against Botrytis cinerea Rhizoctonia solani . Surprisingly, absence led enhanced lateral root formation Arabidopsis thaliana during interaction with Transcriptomic analysis co-regulation adjacent genes, including candidates coding C3H1-type zinc finger protein lytic polysaccharide monooxygenase, suggesting coordination between environmental response mechanisms. Conclusion Our findings establish essential providing novel insights into one compounds this mycoparasite. These may pave way development improved agents application potent biopesticide contributing eco-friendly sustainable disease management.

Language: Английский

Citations

0

Assessment of the chemical composition of buriti (Mauritia flexuosa Liliopsida) and cassava (Manihot esculenta Crantz) residues and their possible application in the bioproduction of coconut aroma (6 pentyl-α-pyrone) DOI
Adriana Silva do Nascimento, Ulisses Magalhães Nascimento, Germildo Juvenal Muchave

et al.

Bioprocess and Biosystems Engineering, Journal Year: 2024, Volume and Issue: 47(10), P. 1633 - 1645

Published: July 6, 2024

Language: Английский

Citations

1

Bacuri and macaxeira waste: physical-chemical characterization and production of coconut bioaroma by solid-state fermentation DOI Creative Commons
Adriana Silva do Nascimento, Selma Gomes Ferreira Leite, Ulisses Magalhães Nascimento

et al.

Revista Brasileira de Ciências Ambientais, Journal Year: 2024, Volume and Issue: 59

Published: Jan. 1, 2024

Agro-industrial waste is considered a global concern. Many of these residues are composed considerable amounts lipids and starch that can potentially be applied in bioprocesses, as the case with from bacuri fruit (Platonia insignis Mart.) sweet cassava (Manihot esculenta Crantz), practically unexploited bioproduction aromas. This work aimed to characterize evaluate coconut aroma 6-pentyl-α-pyrone solid-state fermentation using fungi Trichoderma harzianum. The underwent characterization. Fermentations were conducted under different humidification conditions (water, nutrient solution without additives, solutions glucose or sucrose) for nine days. Aromatic compounds extracted by solid-phase microextraction subsequently quantified gas chromatography. Analyses residue revealed presence some nutritional potential fungus. Still, inhibition halo detected proved others, such resinous derivatives probably responsible lack growth bioproduction. In cassava, not inhibitors had low production, exceeding 7 ppm (weight/weight). Strategically, mixed and, sucrose, maximum production 202.46±1.30 (w/w) was achieved, proving an excellent alternative. Cassava served environment easy germination fungal spores bacuri, important source bioexploitation nutrients, especially lipids, resulting increased 6-pentyl-α-pyrone.

Language: Английский

Citations

0

Light Regulates Secreted Metabolite Production and Antagonistic Activity in Trichoderma DOI Creative Commons
Edgardo Ulises Esquivel‐Naranjo,

Hector Mancilla-Diaz,

Rodolfo Márquez

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 11(1), P. 9 - 9

Published: Dec. 26, 2024

Secondary metabolism is one of the main mechanisms Trichoderma uses to explore and colonize new niches, 6-pentyl-α-pyrone (6-PP) an important secondary metabolite in this process. This work focused on standardizing a method investigate production 6-PP. Ethanol ethyl acetate were both effective solvents for quantifying 6-PP solution had limited solubility potato–dextrose–broth media. The extraction using provided rapid efficient process recover metabolite. was readily produced during development atroviride growing dark, but light suppressed its production. purified, spectrum by nuclear magnetic resonance mass spectroscopy identical that commercial Light also induced or other unidentified metabolites several species Trichoderma. antagonistic activity T. influenced light, as suppression plant pathogens greater dark. secreted potato–dextrose–agar differentially regulated indicating with against pathogens. has influence Trichoderma, trait key relevance selecting strains protection.

Language: Английский

Citations

0