The evaluation of the effect of biological control agents and melatonin against fusarium oxysporum infecting potatoes. DOI Open Access

Londeka Akhona Mbatha

Published: Jan. 1, 2023

Potatoes (Solanum tuberosum L.) are essential to the South African agricultural industry. Postharvest diseases, including Fusarium dry rot (FDR) caused by oxysporum (S.), detrimental potato industry, resulting in yield and economic losses. Currently, fungicides primary means of controlling disease commercially. However, use synthetic chemicals has reduced their efficacy against some strains. Therefore, there is a need for safer, more sustainable, environmentally friendly control methods management postharvest fungal diseases such as FDR. Biological agents (BCAs) generally regarded safe (GRAS) products have inhibitory effects various pathogensand can be used alternatives chemicals. The aim study was evaluate biological (yeast bacteria) melatonin, individually combination, F. vitro vivo. To potential BCAs, 30 isolates were obtained from exocarps leaves crops, namely, potatoes(Solanum tuberosum), sweet potatoes (Ipomoea batatas), erect prickly pear (Opuntia stricta), Ganoderma mushrooms (Ganoderma resinaceum, austroafricanum,) tomatoes lycopersicum). screened using streaking method. Isolates NG1, NG2, NG3, NG4, KG1 inhibited growth on dextrose agar than 50% selected secondary screening. In screening, NGI (60%) NG2 (62%) significantly mycelial PDA compared control. vivo, NG1 severity FDR inoculated 'Sifra' tubers had percentages 47,2% 46,1%, respectively, untreated BLAST prediction identified isolate bacterial species Burkholderia cenocepacia (strain LMG 16656) Bacillus amyloliquefaciens MPA 1034). melatoninwas determined screening six concentrations (0 M; 1 10 15 50 M 100M) phytohormone disc fusion best- performing melatonin progressed vivo Melatonin concentration 100 highest inhibition percentage (40,83%) second third best with 35,83% 34,17%, respectively. an antifungal agent, lower results. treated showed lowest 58,57%, followed 59,54% 60,31%, Subsequently, integration best-performing BCAs evaluated. (Burkholderia amyloliquefaciens) integrated vitro, treatment Bamy + MEL100 (59,92%), MEL15 MEL50 56,12% 55,27%, 50,61% after 9 days at 25℃. Bcen exhibited 52,63% 59,72%, Overall, combination B. less severe symptoms across all concentrations. Furthermore, exogenous application quality parameters treatments administered dipping method stored ambient temperature 14 days. sampled prepared phenolic, protein, ascorbic acid determination assays. combined most effective phenolic content (144,1 mg GAE/g DW), protein (68 mg/g DM). Tubers (5,48 AAE/100g Findings indicated that do not adverse tubers. This demonstrated first time, agent causing effectiveness antagonistic microorganism, oxysporum.

Language: Английский

Identification and Pathogenicity of Fusarium Fungi Associated with Dry Rot of Potato Tubers DOI Creative Commons
Olga Gavrilova, А. С. Орина,

Ilya I. Trubin

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 598 - 598

Published: March 16, 2024

Dry rot of potato tubers is a harmful disease caused by species the Fusarium genus. Studies on composition and features spp. that cause in Russia are limited. Thirty-one strains belonging to F. sambucinum complex (FSAMSC) solani (FSSC) were accurately identified using multilocus phylogenetic analysis tef rpb2 loci, their physiological characteristics studied detail. As result, 21 s. str. 1 strain venenatum within FSAMSC identified. Among analyzed FSSC, one mori, four noneumartii, two both stercicola vanettenii This first record mori as novel host plant, detection noneumartii Russia. The clear optimal temperature for growth was noted be 25 °C, with rate 11.6–15.0 mm/day, whereas, range between 30 5.5–14.1 mm/day. distinctive ability grow at 5 °C has been demonstrated. All pathogenic cv. Gala extensive damage tuber tissue an incubation 23 month. fungi FSAMSC, more aggressive 23.9 ± 2.2 mm necrosis average compared strain—17.7 1.2 mm. most 32.2 0.8 average. aggressiveness stercicola, especially significantly lower: sizes 17.5 0.5 mm, 17.2 0.2 12.5 1.7 respectively. At only necroses 6.7 0.5–15.9

Language: Английский

Citations

6
Evaluation of Fusarium Head Blight in Wheat Associated With Agronomic Practices in Southwest and Central Ethiopia DOI Creative Commons
Issa Eibrahim Umer,

Fikre Lemessa Ocho,

Daniel Teshome Lopisso

et al.

Advances in Agriculture, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Fusarium head blight (FHB) is caused primarily by graminearum ( Gibberella zeae L.) and a major problem to wheat production in Ethiopia. It directly affects the yield of indirectly contaminates products due mycotoxins. A field survey was conducted Central Southwest Ethiopia assess intensity FHB its association with agronomic practices during 2019/2020 cropping season. total 144 fields 12 districts were surveyed analysed using GENMOD technique SAS. Logistic regression used test factors. The result showed that prevalent all widely distributed 100% incidence some fields. epidemic favoured areas humid weather, most notably includes Tello, Deddo, Mencho, She‐Bench Adiyo Ethiopia, but suppressed Endegagn, Soddo Wolisso These results confirm severely affected than High (≥75%) severity index (≥55%) associated potato, teff maize as preceding crops; less frequent ploughing; late planting time (September beyond it); Kakaba’a’, Kingbird Mekuye recently sown varieties. Low had high probability July, peas beans crops Triticale, Hidase Shorima study suggests use crop, ploughing, infected varieties July could be designing integrated disease management options reduce wheat.

Language: Английский

Citations

0
Genome-Wide Identification and Expression Profiling of Potato (Solanum tuberosum L.) Universal Stress Proteins Reveal Essential Roles in Mechanical Damage and Deoxynivalenol Stress DOI Open Access
Tianshuai Qi,

Fumeng He,

Xinqi Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1341 - 1341

Published: Jan. 22, 2024

Universal stress proteins (USPs) play an important regulatory role in responses to abiotic stress. Most of the research related USPs so far has been conducted on plant models such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa L.), and cotton (Gossypium hirsutum L.). The potato (Solanum tuberosum L.) is one four major food crops world. susceptible mechanical damage infection by pathogenic fungi during transport storage. Deoxynivalenol (DON) released Fusarium can seriously degrade quality potatoes. As a result, it great significance study expression pattern StUSP gene family under conditions. In this study, total 108 USP genes were identified from genome Atlantic potato, divided into subgroups. Based their genetic structure, physical chemical properties other aspects biological characteristics are comprehensively analyzed. Collinear analysis showed that homologous StUSPs representative species lycopersicum, Arabidopsis, Oryza L., Nicotiana attenuata) highly conserved. cis-regulatory elements promoter involved hormones, environmental stress, damage, light response. RNA-seq there differences patterns members each subgroup different stresses. A Weighted Gene Coexpression Network Analysis (WGCNA) central differential coexpression mainly plant–pathogen response process, hormone signal transduction, biosynthesis process secondary metabolites. Through qRT-PCR analysis, was confirmed StUSP13, StUSP14, StUSP15, StUSP41 may be candidate adversity results provide basis for further functional potatoes

Language: Английский

Citations

3
Mycotoxin Accumulation in Dry Rot Potato Tubers from Algeria and Toxigenic Potential of Associated Isolates of Fusarium Genus DOI

Sihem Hadjebar,

Amine Yekkour, Nadjette Djemouaı

et al.

Current Microbiology, Journal Year: 2024, Volume and Issue: 81(10)

Published: Aug. 23, 2024

Language: Английский

Citations

1
Impact of fungicides on potato pathogen in the Tambov region of the Russian Federation DOI Creative Commons
Marina Lyashko, Francess Sia Saquee, Simbo Diakite

et al.

RUDN Journal of Agronomy and Animal Industries, Journal Year: 2024, Volume and Issue: 19(1), P. 111 - 121

Published: Jan. 30, 2024

Fungicide application has been reported to effectively manage fungal disease that causes 10 80 % of the average annual estimated loss, including management costs potatoes during production cycle in Russia. The agricultural losses caused by these pathogens are highly significant. A field experiment was conducted for two consecutive years (2022 and 2023), using a potato varietal line fungicides (Zummer Shirlan) assess efficiency newly introduced previously used on Phytophthora infestans (potato blight), Rhizoctonia solani (Black scurf), Streptomyces scabies (Common scab), Fusarium spp. (Fusarium dry rot). arranged randomized complete block design with four replicates. Generally, treatment 5 exhibited highest pathogen severity population lowest fresh tuber yield potatoes. Treatments 2 4 (Zimmer Shirlan at 0.4 L/ha) concentrations had crop studied experiment. Both treatments statistically similar high tolerance pressure, contributing an increase 10.25 t/ha. Treatment 5, which population, 9.36 This research demonstrated Zimmer significantly lowered interaction all diseases studied. Therefore, study confirmed spraying intervals concentration rate L/ha within developmental stages (budding, beginning flowering, end flowering) reduces development, damage diseases, increases yield.

Language: Английский

Citations

0
Characterization of three novel stem rot pathogens and their antagonistic endophytic bacteria associated with Cistanche deserticola DOI
Xinyi Zhou,

Ya-Li Ban,

Chengbin Wang

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 8, 2024

Language: Английский

Citations

0
Identification and Phylogeny of Fusarium falciforme causing Potato Dry rot, its Silver Nanoparticles Synthesis, Characterization and Use against Bacterial Pathogens DOI Creative Commons
Syed Haseeb Shah, Xiaoliang Shan, Sofia Baig

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 31, 2024

Abstract Potato tubers collected from different areas showed the prevalence of dry rot with characteristic white mycelia Fusarium pathogen. species on diseased were isolated based morphological features. 18S-ITS analysis followed by Phylogenetic tree constructed using TEF1-α identified isolates as Fusarium verticilloides, soloni, oxysporum and falciforme. According to our knowledge, this study is first report occurrence falciforme causing potato in Pakistan. The pathogenicity test confirmed reappearance symptoms tuber. Similarly, formation Ag NPs was UV, XRD, FTIR SEM. synthesized color change accordingly UV peak observed at 430 nm. XRD revealed specific angles 36.14°, 44.26°, 64.42° 77.44° corresponding face cubic structure. band absorption 1413 cm1, 1041 cm1 690 ascribed presence various biomolecules O-H N-H stretching vibrations synthesis. SEM indicated EDS NPs. Maximum zone inhibition against Pseudomonas aeruginosa, Escherichia coli, Pseudomonas syringae Staphylococcus aureus 0.05 LSD significance.

Language: Английский

Citations

0
Discover the Most Effective Disease Management Strategies for Fusarium Dry Rot of Potato through Comprehensive Bio-assay of Three Techniques (Chemical, Plant extracts, and Bio-control) DOI Creative Commons
Elias Mjaika Ndifon

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 5, 2024

Potato (Solanum tuberosum) is a multicultural staple food and cash crop. Unfortunately, production of potatoes predominantly constrained by diseases including dry rots. To evaluate management rots, three in vitro sub-trials were set up using the completely randomized design with each treatment replicated thrice for trial. Firstly, synthetic fungicides assayed against Fusarium oxysporum f.sp. tuberosi. Secondly, plant extracts assessed F. oxysporum. Finally, efficacy Trichoderma harzianum applied was evaluated. The colony radii measured. inhibition Ketoconazole (at 100% concentration) significantly (p≤0.05) highest, followed (50% concentration), Itraconazole (100% Sulphur Ridomil finally concentration). Percentage growth species ranged from 39.5-95.7%. Blue gum (Eucalyptus globulus) gave highest inhibition, blue Sweet alligator-pepper (Aframomum melegueta) at concentration, locust bean (Parkia biglobosa) candle bush (Senna alata) Senna alata descending order percentage inhibition. Plant caused fungus between 20.6-100% time. Inhibition T. Harzianum isolate BGMZ4 NSBM then BGMZ3. Control Oxysporum 23.5-94.1% All methods evaluated successfully inhibited pathogen compared to control.

Language: Английский

Citations

0
THE EFFECT OF THE INOCULATION METHOD ON THE VIRULENCE OF PATHOGENS CAUSING DRY ROT OF POTATO DOI

Anastasiya Nikolaeva,

S Hvorova,

Marat Lutfullin

et al.

Published: Nov. 11, 2024

Isolates of micromycetes from the genus Fusarium identified as strains F. oxysporum, solani and redolens were isolated potatoes with dry rot. The effect inoculation method (spores or mycelium) on virulence was investigated potato tubers Zumba Dogoda varieties. It found that only one isolate out six showed varying degrees depending infection

Language: Английский

Citations

0
Enhancing Disease Resistance in Plants and Stored Potato Tubers through Inoculation of Seed Tubers with the Endophyte Bacillus subtilis 10-4 and Genomic Analysis of its Antimicrobial Properties DOI
Л. И. Пусенкова, Oksana Lastochkina

Applied Biochemistry and Microbiology, Journal Year: 2024, Volume and Issue: 60(6), P. 1338 - 1350

Published: Dec. 1, 2024

Language: Английский

Citations

0