The evaluation of the effect of biological control agents and melatonin against fusarium oxysporum infecting potatoes. DOI Open Access

Londeka Akhona Mbatha

Published: Jan. 1, 2023

Potatoes (Solanum tuberosum L.) are essential to the South African agricultural industry. Postharvest diseases, including Fusarium dry rot (FDR) caused by oxysporum (S.), detrimental potato industry, resulting in yield and economic losses. Currently, fungicides primary means of controlling disease commercially. However, use synthetic chemicals has reduced their efficacy against some strains. Therefore, there is a need for safer, more sustainable, environmentally friendly control methods management postharvest fungal diseases such as FDR. Biological agents (BCAs) generally regarded safe (GRAS) products have inhibitory effects various pathogensand can be used alternatives chemicals. The aim study was evaluate biological (yeast bacteria) melatonin, individually combination, F. vitro vivo. To potential BCAs, 30 isolates were obtained from exocarps leaves crops, namely, potatoes(Solanum tuberosum), sweet potatoes (Ipomoea batatas), erect prickly pear (Opuntia stricta), Ganoderma mushrooms (Ganoderma resinaceum, austroafricanum,) tomatoes lycopersicum). screened using streaking method. Isolates NG1, NG2, NG3, NG4, KG1 inhibited growth on dextrose agar than 50% selected secondary screening. In screening, NGI (60%) NG2 (62%) significantly mycelial PDA compared control. vivo, NG1 severity FDR inoculated 'Sifra' tubers had percentages 47,2% 46,1%, respectively, untreated BLAST prediction identified isolate bacterial species Burkholderia cenocepacia (strain LMG 16656) Bacillus amyloliquefaciens MPA 1034). melatoninwas determined screening six concentrations (0 M; 1 10 15 50 M 100M) phytohormone disc fusion best- performing melatonin progressed vivo Melatonin concentration 100 highest inhibition percentage (40,83%) second third best with 35,83% 34,17%, respectively. an antifungal agent, lower results. treated showed lowest 58,57%, followed 59,54% 60,31%, Subsequently, integration best-performing BCAs evaluated. (Burkholderia amyloliquefaciens) integrated vitro, treatment Bamy + MEL100 (59,92%), MEL15 MEL50 56,12% 55,27%, 50,61% after 9 days at 25℃. Bcen exhibited 52,63% 59,72%, Overall, combination B. less severe symptoms across all concentrations. Furthermore, exogenous application quality parameters treatments administered dipping method stored ambient temperature 14 days. sampled prepared phenolic, protein, ascorbic acid determination assays. combined most effective phenolic content (144,1 mg GAE/g DW), protein (68 mg/g DM). Tubers (5,48 AAE/100g Findings indicated that do not adverse tubers. This demonstrated first time, agent causing effectiveness antagonistic microorganism, oxysporum.

Language: Английский

Mycotoxin production by Fusarium species and a recent deep insight into management through biocontrol approaches DOI Creative Commons
Amjad Ali, Fatih Ölmez, Muhammed Tatar

et al.

Journal of Global Innovations in Agricultural Sciences, Journal Year: 2023, Volume and Issue: 11(4), P. 455 - 480

Published: Dec. 22, 2023

Fusarium infection has become a significant and widespread concern in modern agriculture, posing substantial threat to numerous essential crop plants. The pernicious fungal pathogen been causing destruction diverse areas, resulting severe consequences for both food security economic stability. Mycotoxins, which are secondary metabolites produced by wide variety of fungi, present serious risks the health humans animals have potential result considerable financial losses agricultural industries. genus is particularly infamous its capacity produce range mycotoxins. summarized study highlights recent progression fusarium towards various pivotal plants with their life cycle, morphological microscopic characteristics, pathogenicity factors. species well-known ability mycotoxins, such as zearalenones, fumonisins, Trichothecenes, Deoxynivalenol, Nivalenol, T-2 toxins detrimental that contaminate products, particular emphasis on grains cereals. new emerging mycotoxins producing challenges control human health. identification implementation efficient biocontrol strategies detoxify through Trichoderma, Plant Secondary Metabolites, Lactic Acid Bacteria, Edible Mushrooms, Enzymes measures guaranteeing safety protecting public mentioned approaches also discussed addressed. Keywords: factors, trichothecenes, agents, enzymatic detoxification.

Language: Английский

Citations

1
None DOI Creative Commons
Amjad Ali, Muhammed Tatar,

Irum T abbasum

et al.

Journal of Global Innovations in Agricultural and Social Sciences, Journal Year: 2014, Volume and Issue: 2(3)

Published: Sept. 1, 2014

countries, causing devastating impacts on timber trees in the forest (Suga and Hyakumachi, 2004).Fusarium species are associated swith production of different kinds secondary metabolites, such as gibberellin zearalenone, which most significant plant growth regulators also used to accelerate animals (Nesic Nesic, 2013;Qiu et al., 2020).Trichothecenes fumonisins produced by these pathogenic humans can cause mycotoxicosis when they fed contaminated food (Hao 2022; Li 2020).Fusarium spp.can survive dead decaying material, sustain itself soil for up 16 years absence a host, acts an important biodegrading agent (Early 2009).

Language: Английский

Citations

0
The evaluation of the effect of biological control agents and melatonin against fusarium oxysporum infecting potatoes. DOI Open Access

Londeka Akhona Mbatha

Published: Jan. 1, 2023

Potatoes (Solanum tuberosum L.) are essential to the South African agricultural industry. Postharvest diseases, including Fusarium dry rot (FDR) caused by oxysporum (S.), detrimental potato industry, resulting in yield and economic losses. Currently, fungicides primary means of controlling disease commercially. However, use synthetic chemicals has reduced their efficacy against some strains. Therefore, there is a need for safer, more sustainable, environmentally friendly control methods management postharvest fungal diseases such as FDR. Biological agents (BCAs) generally regarded safe (GRAS) products have inhibitory effects various pathogensand can be used alternatives chemicals. The aim study was evaluate biological (yeast bacteria) melatonin, individually combination, F. vitro vivo. To potential BCAs, 30 isolates were obtained from exocarps leaves crops, namely, potatoes(Solanum tuberosum), sweet potatoes (Ipomoea batatas), erect prickly pear (Opuntia stricta), Ganoderma mushrooms (Ganoderma resinaceum, austroafricanum,) tomatoes lycopersicum). screened using streaking method. Isolates NG1, NG2, NG3, NG4, KG1 inhibited growth on dextrose agar than 50% selected secondary screening. In screening, NGI (60%) NG2 (62%) significantly mycelial PDA compared control. vivo, NG1 severity FDR inoculated 'Sifra' tubers had percentages 47,2% 46,1%, respectively, untreated BLAST prediction identified isolate bacterial species Burkholderia cenocepacia (strain LMG 16656) Bacillus amyloliquefaciens MPA 1034). melatoninwas determined screening six concentrations (0 M; 1 10 15 50 M 100M) phytohormone disc fusion best- performing melatonin progressed vivo Melatonin concentration 100 highest inhibition percentage (40,83%) second third best with 35,83% 34,17%, respectively. an antifungal agent, lower results. treated showed lowest 58,57%, followed 59,54% 60,31%, Subsequently, integration best-performing BCAs evaluated. (Burkholderia amyloliquefaciens) integrated vitro, treatment Bamy + MEL100 (59,92%), MEL15 MEL50 56,12% 55,27%, 50,61% after 9 days at 25℃. Bcen exhibited 52,63% 59,72%, Overall, combination B. less severe symptoms across all concentrations. Furthermore, exogenous application quality parameters treatments administered dipping method stored ambient temperature 14 days. sampled prepared phenolic, protein, ascorbic acid determination assays. combined most effective phenolic content (144,1 mg GAE/g DW), protein (68 mg/g DM). Tubers (5,48 AAE/100g Findings indicated that do not adverse tubers. This demonstrated first time, agent causing effectiveness antagonistic microorganism, oxysporum.

Language: Английский

Citations

0