The Changing Immune Landscape of Innate-like T Cells and Innate Cells Throughout Life DOI Open Access

Marziyeh Taheri,

Christopher Menne,

Jeremy Anderson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 17, 2024

ABSTRACT Spectral flow cytometry is an advanced immunological tool that enables comprehensive analysis of the immune system by simultaneously comparing innate and adaptive cells. Here, using a 40-colour antibody panel we advance our knowledge innate-like T cells investigating chemokine receptors, activation maturation markers not usually assessed on these populations examine age-related effects to cell subsets. We characterised phenotypic changes peripheral blood mononuclear (PBMC) in three age groups: newborn (cord blood), adults aged 20-30 years, 70-80 focusing cells, including MAIT NKT γδ ILCs, Natural Killer (NK) identify subsets double-negative (DN) (CD4 - CD8 ) CD161 + increase manner exhibit phenotype similar Innate-like express patterns receptors CCR4, CCR6, CD27, CD38, CD57 CD45RA, resemble memory conventional CD4 could detect ILCs all ranges, although frequency ILC1, ILC2, ILC3 decreased with age. Notably, NK marker, CD57, as universal marker defines ageing This study enhances understanding ontogeny human highlighting significant

Language: Английский

The Changing Immune Landscape of Innate-like T Cells and Innate Cells Throughout Life DOI Open Access

Marziyeh Taheri,

Christopher Menne,

Jeremy Anderson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 17, 2024

ABSTRACT Spectral flow cytometry is an advanced immunological tool that enables comprehensive analysis of the immune system by simultaneously comparing innate and adaptive cells. Here, using a 40-colour antibody panel we advance our knowledge innate-like T cells investigating chemokine receptors, activation maturation markers not usually assessed on these populations examine age-related effects to cell subsets. We characterised phenotypic changes peripheral blood mononuclear (PBMC) in three age groups: newborn (cord blood), adults aged 20-30 years, 70-80 focusing cells, including MAIT NKT γδ ILCs, Natural Killer (NK) identify subsets double-negative (DN) (CD4 - CD8 ) CD161 + increase manner exhibit phenotype similar Innate-like express patterns receptors CCR4, CCR6, CD27, CD38, CD57 CD45RA, resemble memory conventional CD4 could detect ILCs all ranges, although frequency ILC1, ILC2, ILC3 decreased with age. Notably, NK marker, CD57, as universal marker defines ageing This study enhances understanding ontogeny human highlighting significant

Language: Английский

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