Influence of Sex and a High-Fiber Diet on the Gut Microbiome of Alentejano Pigs Raised to Heavy Weights

Veterinary Sciences, Journal Year: 2023, Volume and Issue: 10(11), P. 641 - 641

Published: Nov. 2, 2023

This study investigates the influence of sex and a dietary transition on gut microbiota local Portuguese pig breed. Three groups male Alentejano pigs (n = 10 each) were raised between ~40 160 kg LW. Group C included that surgically castrated, while I group intact ones; both fed with commercial diets. The third group, IExp, diets until ~130 kg, then replaced by an experimental diet based legumes agro-industrial by-products kg. Fecal samples collected two weeks before slaughter. total DNA was extracted used for 16S metabarcoding MiSeq® System. from to substantially increased shifted diversity observed. Complex carbohydrate fermenting bacteria, such as Ruminococcus spp. Sphaerochaeta spp., significantly more abundant in IExp (q < 0.05). On other hand, castrated presented lower abundance potential probiotic, Roseburia Lachnospiraceae NK4A136 0.01), bacteria commonly associated better health body fat composition. Understanding role is paramount ensure low skatole deposition consumers’ acceptance pork products non-castrated pigs.

Language: Английский

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Antibiotic treatment induces microbiome dysbiosis and reduction of neuroinflammation following traumatic brain injury in mice

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Background The gut microbiome is linked to brain pathology in cases of traumatic injury (TBI), yet the specific bacteria that are implicated not well characterized. To address this gap, study, we induced (TBI) male C57BL/6J mice using controlled cortical impact (CCI) model. After 35 days, administered a broad-spectrum antibiotics (ABX) cocktail (ampicillin, gentamicin, metronidazole, vancomycin) through oral gavage for 2 days diminish existing microbiota. Subsequently, inflicted second TBI on and analyzed neuropathological outcomes five later. Results Longitudinal analysis showed significant shifts diversity abundance bacterial genera during both acute chronic inflammation. These changes were particularly dramatic following treatment with ABX after TBI. did affect production short-chain fatty acids (SCFA) but alter intestinal morphology, characterized by reduced villus width lower count goblet cells, suggesting potential negative impacts integrity. Nevertheless, diminishing damage, apoptotic cell density, microglial/macrophage activation thalamic regions brain. Conclusions Our findings suggest eliminating colonized via reduces neuroinflammation enhances neurological despite implications health.

Language: Английский

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Combined gut microbiome and metabolomics to reveal the mechanism of proanthocyanidins from the roots of Ephedra sinica Stapf on the treatment of ulcerative colitis

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 249, P. 116351 - 116351

Published: July 10, 2024

Language: Английский

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Comparison of the Skin Microbiota in the Periocular Region between Patients with Inflammatory Skin Diseases and Healthy Participants: A Preliminary Study

Life, Journal Year: 2024, Volume and Issue: 14(9), P. 1091 - 1091

Published: Aug. 30, 2024

(1) Background: Periocular or periorbital dermatitis is a common term for all inflammatory skin diseases affecting the area of around eyes. The clear etiopathogenesis periocular still not fully understood. Advances in molecular techniques studying microorganisms living and on our bodies have highlighted microbiome as possible contributor to disease, well promising diagnostic marker target innovative treatments. aim this study was compare composition diversity microbiota region between healthy individuals affected by specific entity dermatitis. (2) Methods: A total 35 patients with 39 controls were enrolled study. After swab from taken participants, DNA extraction 16S rRNA gene amplicon sequencing using Illumina NovaSeq technology performed. (3) Results:

Language: Английский

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Positive impact of early-probiotic administration on performance parameters, intestinal health and microbiota populations in broiler chickens

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104401 - 104401

Published: Oct. 10, 2024

Minimizing the utilization of antibiotics in animal production is crucial to prevent emergence antimicrobial resistances. Thus, research on alternatives needed maintain productivity, sustainability, and health. To gain a comprehensive understanding probiotics' modes action performance, intestinal microbiota, gut health poultry, 3 probiotic strains (Enterococcus faecalis CV1028 [EntF], Bacteroides fragilis GP1764 [BacF], Ligilactobacillus salivarius CTC2197 [LacS]) were tested 2 vivo trials. Trial 1 comprised negative control group fed basal diet (BD) treatment groups that received BD with EntF, BacF LacS. included group, positive Zinc-Bacitracin as antibiotic growth promoter (AGP), treated blend probiotics (EntF+BacF+LacS) during 0 10 or 35 d, respectively. Wheat-soybean-rye based diets without exogenous enzymes used challenge model induce mild- moderate-inflammatory process gut. In 1, individually administered improved FCR at 8 d compared Control, but these effects lost following growing periods probably due high grade challenging too low dose probiotics. 2, both Probiotic treatments, only significantly same extent Antibiotic end trial. Although performance between mixture showed similar values, microbiota analysis revealed different microbial composition 7 not 21 d. This suggests AGP differ their microbiome, changes observed first days' posthatch are relevant study. Therefore, administration was proven sufficient improvements those birds promoters throughout whole experimental

Language: Английский

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