The association of TNF-alpha secretion and mtDNA copy number in CD14+ monocytes of patients with obesity and CHD

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: March 20, 2024

Introduction Mitochondrial dysfunction may be one of the causes inflammatory activation monocytes and macrophages, which leads to excessive secretion mediators development chronic inflammation. Aims The study was aimed evaluate cytokine tumor necrosis factor-α (TNF-α) in primary culture monocytes, analyze its relationship with number mitochondrial DNA (mtDNA) copies blood patients coronary heart disease (CHD) obesity. Materials methods 108 obesity concomitant CHD a control group 25 participants were included study. CD14 + isolated by standard method ficoll-urographin gradient, followed separation using magnetic particles. mtDNA estimated qPCR. Results It demonstrated that significantly increased groups comparison group. copy positively correlated basal LPS-stimulated TNF-α secretion, most significant correlation found Conclusion Thus, change indicates presence dysfunction, confirm direct involvement mitochondria violation response revealed this as an TNF-α.

Language: Английский

RNA sequencing analysis of early-stage atherosclerosis in vascular-on-a-chip and its application for comparing combustible cigarettes with heated tobacco products

Current Research in Toxicology, Journal Year: 2024, Volume and Issue: 6, P. 100163 - 100163

Published: Jan. 1, 2024

Our previous study showed promising results in replicating early-stage atherosclerosis when vascular endothelial cells (VECs) were exposed to cigarette smoke (CS) extract via M0 macrophages. We used an organ-on-a-chip system as alternative animal testing model atherosclerosis, which is a complex disease involving and immune cell communications. By incorporating macrophages into the vascular-on-a-chip system, we aimed mimic indirect effects of inhalable substances, such CS, on VECs. In current study, further examined suitability our

Language: Английский

Exploring the Association of Biochemical Characterization and Genetic Determinants of TNF-α, CXCR2, and CCR5 Delta 32 Mutation with Predisposition to Polycystic Ovary Syndrome

Life, Journal Year: 2024, Volume and Issue: 14(8), P. 949 - 949

Published: July 28, 2024

PCOS is a heterogeneous, multifactorial endocrine disorder with complex pathophysiology. It globally rising infertility that affects large percentage of women reproductive age, relatively high prevalence 8–13%. Genome-wide association studies have revealed associations genetic variations many diseases, including PCOS. The cellular activity IL8 mediated by the receptor CXCR2, and transcription controlled TNF-α. Therefore, this study aimed to investigate TNF-α, CCR5-delta32, CXCR2 gene Methodology: In case control study, we used amplification-refractory mutation system (ARMS)-PCR detect determine presence polymorphic variants in subjects. These polymorphs may serve as critical candidate pathogenesis therapeutics. Results: case–control study’s findings majority biochemical serum biomarkers examined investigation—including lipids (LDL, HDL, cholesterol), T2DM markers (fasting glucose, free insulin, HOMA-IR), hormones (FSH, LH, testosterone, progesterone)—exhibited statistically significant changes patients. distributions TNF-α (rs1800629), (rs2230054) genotypes analyzed within patients healthy controls considered population were (p < 0.05). heterozygosity CXCR2-CA, GA, CCR5(WT+Δ32*) was significantly associated susceptibility, OR p 0.05 codominant model. Similarly, A allele genes, along CCR5Δ32*(mutant) allele, 0.05. Likewise, (CA+AA) vs CC genotype increased susceptibility PCOS, 2.25, 0.032. Conclusions: Our concludes rs1800629G>A, CXCR2-rs2230054C>T, CCR5-Delta32 rs333 are potential loci for developing Tabuk population. might eventually be useful identifying classifying those who at risk To validate these results, it advised further longitudinal conducted diverse ethnic populations larger sample sizes.

Language: Английский