This
study
utilized
Escherichia
coli
bacteriophage
MS2
as
a
surrogate
to
evaluate
the
survival
of
enteric
viruses
in
oyster
meat.
The
phage
was
propagated
its
host,
coli,
spiked
into
meat
at
concentration
8.6
x10¹⁰
PFU/ml
(10.93
log
CFU/ml)
and
stored
under
chilled
conditions
on
ice
-20°C
(frozen
storage).
Phage
titres
were
measured
using
soft
agar
overlay
method,
with
sampling
conducted
every
second
day
for
ice-stored
fifth
frozen-stored
analysis
demonstrated
that
phages
by
extension
viruses,
could
persist
up
10
days
counts
reaching
4.78
PFU/g.
In
frozen
storage,
survived
35
days,
exhibiting
4
reduction
viral
counts.
These
results
highlight
significant
during
both
storage
conditions.
Applied Microbiology,
Journal Year:
2024,
Volume and Issue:
4(2), P. 594 - 606
Published: March 25, 2024
The
transformation
of
the
food
chain
due
to
technological
advances
has
had
significant
implications
in
regard
safety.
A
noteworthy
trend
this
evolution
relates
emergence
new
or
previously
unseen
pathogens
within
products,
thereby
altering
landscape
foodborne
illness
epidemiology.
escalating
frequency
these
events
underscores
need
for
a
comprehensive
re-evaluation
preventive
strategies.
occurrence
novel
species
bacteria,
viruses,
parasites,
and
unusual
biotoxins
from
unexpected
sources
challenged
previous
limits
that
been
set
prevent
outbreaks.
repercussions,
ranging
detrimental
effects
on
public
health
economic
burden,
are
influenced
by
myriad
factors
affecting
emerging
ailments.
Among
shifts
population
demographics
behaviors,
especially
dietary
patterns,
as
well
climate
extremes,
more
precise
pathogen
detection,
microbial
adaptation,
evolving
agricultural
practices,
transformative
changes
industry.
This
review
critically
examines
impact
metamorphosis
along
chain,
encompassing
production,
processing,
handling,
packaging,
storage,
transportation,
industry
dynamics
influencing
pathogens.
Additionally,
potential
solutions
mitigate
manage
issue
proposed.
Human Vaccines & Immunotherapeutics,
Journal Year:
2025,
Volume and Issue:
21(1)
Published: Jan. 16, 2025
While
Hepatitis
A
Virus
(HAV)
vaccination
in
global
immunization
programs
has
shown
a
virtual
elimination
of
the
disease
within
few
years
program,
changing
epidemiological
landscape
India
underscores
need
for
evidence-based,
updated
guidance
on
practices.
In
May
2024,
panel
15
distinguished
opinion
leaders
and
an
organizing
committee
convened
intensive,
face-to-face
advisory
board
meeting
high
burden
HAV
infection
among
adults,
increased
mortality
rate
adolescents,
symptomatic
presentation
children,
evolving
globally
India.
Extensive
comparable
deliberations
long-term
follow-up
data
from
country
origin
advocated
immunogenicity,
tolerability,
protective
effects
single-dose
live
attenuated
vaccine
children.
Finally,
consensus
was
achieved
recognition
attention
toward
prevention
through
coverage.
The
single
dose
important
outcome
this
meeting.
Viruses,
Journal Year:
2025,
Volume and Issue:
17(2), P. 174 - 174
Published: Jan. 26, 2025
Foodborne
viruses
such
as
human
norovirus
(HuNoV)
and
hepatitis
A
virus
(HAV)
are
the
major
causes
of
foodborne
illnesses
worldwide.
These
have
a
low
infectious
dose
persistent
in
environment
food
for
weeks.
Ready-to-eat
(RTE)
moisture
foods
(LMFs)
undergo
minimal
pathogen
reduction
processes.
In
recent
years,
multiple
HAV
outbreaks
involving
hundreds
individuals
were
associated
with
consumption
dates,
indicating
that
they
could
be
important
vehicles
infection.
There
is
no
standard
method
extraction
detection
from
but
herein
we
compared
efficiency
three
different
protocols
based
on
ISO
15216
murine
(MNV)
whole
Medjool
dates
successfully
employed
best
performing
HAV,
HuNoV
GI,
GII
determined
limit
(LOD95)
61,
148,
184
genomic
equivalent
(gEq)
per
25
g,
respectively.
Finally,
tested
adopted
various
varieties
including
pitted
ones
reported
GI
four
naturally
contaminated
date
varieties.
This
protocol
surveillance
purposes
outbreak
management
related
to
dates.
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(4), P. 776 - 776
Published: March 28, 2025
Quantitative
Polymerase
Chain
Reaction
(qPCR)
is
a
molecular
technique
that
has
become
gold
standard
in
various
disciplines,
including
environmental
microbiology,
due
to
its
high
sensitivity
and
specificity.
In
recent
years,
it
been
extensively
used
wastewater-based
epidemiology
monitor
the
prevalence
of
different
viruses
population.
this
study,
we
evaluated
whether
no
inclusion
curve
each
single
experiment
reduce
time
costs
could
have
an
impact
on
accuracy
results.
Thirty
independent
RT-qPCR
experiments
using
quantitative
synthetic
RNA
material
were
conducted
for
seven
viruses,
which
include
two
targets
novel
SARS-CoV-2,
hepatitis
A
E,
noroviruses
genogroups
I
II,
human
astrovirus,
rotavirus.
Results
showed
although
all
presented
adequate
efficiency
rates
(>90%),
variability
was
also
observed
between
them,
independently
viral
concentration
tested.
NoVGII
virus
higher
inter-assay
terms
while
showing
better
sensitivity.
heterogeneity
results,
SARS-CoV-2
highest
rates,
being
N2
gene
largest
(CV
4.38–4.99%)
lowest
(90.97%).
These
findings
indicate
every
recommended
obtain
reliable
npj Viruses,
Journal Year:
2025,
Volume and Issue:
3(1)
Published: April 4, 2025
Foodborne
viruses
are
significant
public
health
threats,
capable
of
causing
life-threatening
infections
and
posing
major
risks
for
future
pandemics.
However,
the
development
vaccines
treatments
remains
limited
due
to
gaps
in
understanding
their
biophysical
properties.
Among
these
viruses,
noroviruses
currently
leading
cause
viral
gastroenteritis
globally
responsible
numerous
foodborne
outbreaks.
In
this
review,
we
explore
use
methods,
with
a
focus
on
atomic
force
microscopy
(AFM),
study
viruses.
We
demonstrate
how
AFM
can
provide
crucial
insights
into
virus-host
interactions,
transmission
dynamics,
environmental
stability.
also
show
that
integration
various
approaches
offers
new
opportunities
advancing
our
ultimately
guiding
effective
prevention
strategies
antiviral
therapies.
