Mechanism of total saponins of Ranunculus ternatus Thunb. in treatment of breast cancer based on liquid chromatography–mass spectrometry and network analysis

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

To explore the mechanism of total saponins Ranunculus ternatus Thunb. (RT) in treatment breast cancer (BC) using liquid chromatography-mass spectrometry (LC-MS) technology and network analysis. The metabolites RT were detected LC-MS. Metabolites targets BC identified different databases, potential pathways predicted protein-protein interaction pathway enrichment analyses. A mouse model created by cellular injection MCF-7 cells used as research objects for vivo vitro validation experiments to study anti-BC RT. Kyoto Encyclopedia Genes Genomes analysis showed that Janus kinase/signal transducer activator transcription signaling might be associated with effects Total from had a good effect can inhibit expression JAK2 STAT3-related proteins mRNA, affect levels serum inflammatory factors tumor necrosis factor-α, interleukin-6, interleukin-10, growth, proliferation, migration, promote cell apoptosis. may play role regulating JAK2/STAT3 pathway.

Language: Английский

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Elucidating the Anti-Diabetic Mechanisms of Mushroom Chaga (Inonotus obliquus) by Integrating LC-MS, Network Pharmacology, Molecular Docking, and Bioinformatics

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(11), P. 5202 - 5202

Published: May 28, 2025

Diabetes mellitus is characterized by insulin resistance, impaired glucose homeostasis, and dysregulated metabolism, leading to complications. Inonotus obliquus (Chaga) has shown potential anti-diabetic effects, but the bioactive compounds molecular targets remain unclear. This study aimed identify components of Chaga elucidate their mechanisms using LC-MS compound screening, network pharmacology, docking, bioinformatics analyses. extract was prepared 95% ethanol, were identified through UHPLC-QE-MS analysis. Target prediction conducted Swiss Prediction SEA databases, while diabetes-related retrieved from GeneCards. A PPI constructed STRING analyzed for GO KEGG enrichment. Molecular docking performed AutoDock Vina, gene expression validated GSE7014 dataset GEPIA database, with immune cell infiltration CIBERSORT. 30 Chaga, including 21 triterpenoids, four flavonoids, two diterpenoids. Network pharmacology predicted 432 targets, 167 core enriched in key pathways, primarily PI3K/Akt signaling pathway. revealed strong binding affinities five seven targets. Bioinformatics analysis significant changes ESR1, IL6, SRC, showed correlations between subtypes. highlights identifying interactions central diabetic Further vitro vivo studies are needed validate these findings.

Language: Английский

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Integrating Metabolomic Analysis, Network Pharmacology, and Molecular Docking to Underlying Pharmacological Mechanism and Ethnobotanical Rationalization for Diabetes Mellitus: Study on Medicinal Plant Fibraurea tinctoria Lour.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Fibraurea tinctoria Lour. has long been used in traditional medicine to treat diabetes mellitus (DM). However, a comprehensive scientific understanding of its potential active compounds and underlying pharmacological mechanisms still needs be unveiled.

Language: Английский

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Edible plant Artemisia argyi H.Lév. & Vaniot: An in vitro study on its anti-Helicobacter pylori effect

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 124, P. 106648 - 106648

Published: Dec. 25, 2024

Language: Английский

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