Peripheral Coding of Sex Pheromones in the Tomato Leaf Miner, Phthorimaea absoluta (Meyrick) DOI
Xiaoyu Hu, Rui Tang,

Limei Song

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Phthorimaea absoluta releases (E3,Z8,Z11)-tetradeca-3,8,11-trienyl acetate (E3,Z8,Z11–14:OAc) and (E3,Z8)-tetradeca-3,8-dienyl (E3,Z8–14:OAc) with a ratio of 90:10 as the sex pheromone. However, how this pest uses pheromone receptors (PRs) to detect two components is unknown. Here, we functionally characterize PR repertoire P. absoluta. First, identified five putative PRs by transcriptome sequencing, i.e., PabsOR4, PabsOR8, PabsOR12a, PabsOR14, PabsOR17. These are predominantly expressed in male antennae. Next, them Drosophila OR67 neurons investigated their responses. PabsOR14 PabsOR8 selpond main component,E3,Z8,Z11–14:OAc different sensitivities, while PabsOR17 tuned minor component, E3,Z8–14:OAc. In addition, PabsOR4 weakly responds both components. Moreover, potently respond non-sex compound, (Z)-7-dodecenyl (Z7–12:OAc). Lastly, demonstrated that Z7–12:OAc can replace E3,Z8–14:OAc attract virgin males. Our findings elucidate peripheral coding absoluta, providing new perspective for controlling it.

Language: Английский

Genome-Wide Identification and Expression Profiling of Odorant-Binding Protein Genes in the Bean Flower Thrips Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) DOI Creative Commons

Gen Xia,

Lang Yang,

Boliao Li

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 212 - 212

Published: Feb. 14, 2025

Megalurothrips usitatus is an economically important vegetable pest. Because of the growing demand for reducing pesticide use on vegetables, new environmentally friendly strategies controlling M. are urgently needed. Insect odorant-binding proteins prospective targets screening odorant attractants pest control. However, very little known about OBP genes in usitatus. Here, we identified 14 OBPs genome using HMMER and BLAST. The chromosomal location showed that these were widely distributed across eight chromosomes. analysis gene protein structure characteristics revealed substantial diversity within family. spatiotemporal expression profiles ten out MusiOBPs displayed male biased expression, which highly expressed antennae, suggesting they may play a crucial role recognition host plant volatiles thrips aggregation pheromones. Notably, only MusiOBP8 was significantly higher female adults, indicating potential involvement reproduction. Moreover, MusiOBP7 MusiOBP13 pupae, their possible immune responses. These results provide foundation further exploration functions

Language: Английский

Citations

0
Expression and sex pheromone-binding characteristics of pheromone-binding protein 3 in Tuta absoluta (Lepidoptera: Gelechiidae) DOI

Xiao-Lan Ou,

Xianya Li,

Bo Xu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106404 - 106404

Published: April 1, 2025

Language: Английский

Citations

0
Transcriptome sequencing of Antheraea pernyi antennae for identification of olfactory-related genes DOI Creative Commons
Xueting Liu,

Shuwei Ma,

Xinxue Zhang

et al.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: May 19, 2025

In insects, the olfactory system governs physiological and behavioral processes by detecting various odorous molecules. Despite its economic importance adaptability, mechanism of Antheraea pernyi remains insufficiently understood, limiting potential for pest management as a model organism. Hence, we aimed to conduct transcriptome sequencing explore olfactory-related genes in antennae, serving most important organ adult A. pernyi. Based on datasets, 1184 differently expressed (DEGs), including 484 upregulated 700 downregulated genes, were identified comparing profiles male female antennae Moreover, 20, 7, 30, 11, 2 candidate encoding odorant-binding proteins (OBPs), chemosensory (CSPs), odorant receptors (ORs), ionotropic (IRs), sensory neuron membrane (SNMPs), respectively, involved pheromone perception, odor binding, pesticide resistance, growth development regulation screened, which both while expression levels these varied significantly between males females. Multiple sequence alignment indicated that six OBPs exhibited typical characteristics, containing conserved Cys residues with C1-X26-30-C2-X3-C3-X41-42-C4-X8-10-C5-X8-C6. All CSPs followed highly pattern four arranged an exact spacing C1-X6-C2-X18-19-C3-X2-C4. Different numbers transmembrane domains found ORs, IRs, SNMPs. addition, several DEGs involve signal transduction underlying chemoreception also from data, guanine nucleotide-binding protein (G protein), cGMP-dependent kinase (PKA), calmodulin-A (CaM-A), mitogen-activated 1 (MAPK1), phospholipase D2 (PLD2). This study enriches gene database pernyi, providing insights into mechanisms crucial mating control, implications enhancing breeding strategies ensuring sustainability silk industry. These findings may serve theoretical foundation better understanding

Language: Английский

Citations

0
Peripheral Coding of Sex Pheromones in the Tomato Leaf Miner, Phthorimaea absoluta (Meyrick) DOI
Xiaoyu Hu, Rui Tang,

Limei Song

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Phthorimaea absoluta releases (E3,Z8,Z11)-tetradeca-3,8,11-trienyl acetate (E3,Z8,Z11–14:OAc) and (E3,Z8)-tetradeca-3,8-dienyl (E3,Z8–14:OAc) with a ratio of 90:10 as the sex pheromone. However, how this pest uses pheromone receptors (PRs) to detect two components is unknown. Here, we functionally characterize PR repertoire P. absoluta. First, identified five putative PRs by transcriptome sequencing, i.e., PabsOR4, PabsOR8, PabsOR12a, PabsOR14, PabsOR17. These are predominantly expressed in male antennae. Next, them Drosophila OR67 neurons investigated their responses. PabsOR14 PabsOR8 selpond main component,E3,Z8,Z11–14:OAc different sensitivities, while PabsOR17 tuned minor component, E3,Z8–14:OAc. In addition, PabsOR4 weakly responds both components. Moreover, potently respond non-sex compound, (Z)-7-dodecenyl (Z7–12:OAc). Lastly, demonstrated that Z7–12:OAc can replace E3,Z8–14:OAc attract virgin males. Our findings elucidate peripheral coding absoluta, providing new perspective for controlling it.

Language: Английский

Citations

1