Digital Light Processing 3D Printing for Microfluidic Chips with Enhanced-resolution via Dosing- and Zoning-controlled vat Photopolymerization DOI Creative Commons
Huanan Wang,

Zhiming Luo,

Haoyue Zhang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 4, 2023

Abstract Conventional manufacturing techniques to fabricate microfluidic chips like soft lithography and hot embossing processes show limitations including difficulty in preparing multiple-layered structures, cost- labor-consuming fabrication process, low productivity. Digital light processing (DLP) technology has recently emerged as a cost-efficient microfabrication approach for 3D printing of chips, wherein, however, the resolution microchannel is still limited sub-100 microns at best. We hereby developed an innovative DLP strategy high-resolution scalable via dosing- zoning-controlled vat photopolymerization (DZC-VPP). Specifically, we proposed modified mathematical model precisely predict accumulated UV irradiance resin photopolymerization, thereby providing guidance with enhanced resolution. By fine-tuning parameters optical irradiance, exposure time, projection region step distance, can tailor penetration stemming from neighboring layers, therefore avoiding channel blockage due over-exposure or compromised bonding stability owing insufficient curing. Remarkably, this enable biocompatible drop-makers that be used cell encapsulation. In general, current DZC-VPP method major advances precise fabrication, represents significant forward widespread applications microfluidics-based biomedical fields.

Language: Английский

Digital light processing 3D printing for microfluidic chips with enhanced resolution via dosing- and zoning-controlled vat photopolymerization DOI Creative Commons

Zhiming Luo,

Haoyue Zhang,

Runze Chen

et al.

Microsystems & Nanoengineering, Journal Year: 2023, Volume and Issue: 9(1)

Published: Aug. 15, 2023

Conventional manufacturing techniques to fabricate microfluidic chips, such as soft lithography and hot embossing process, have limitations that include difficulty in preparing multiple-layered structures, cost- labor-consuming fabrication low productivity. Digital light processing (DLP) technology has recently emerged a cost-efficient microfabrication approach for the 3D printing of chips; however, resolution microchannels is still limited sub-100 microns at best. Here, we developed an innovative DLP strategy high scalable microchannel by dosing- zoning-controlled vat photopolymerization (DZC-VPP). Specifically, proposed modified mathematical model precisely predict accumulated UV irradiance resin photopolymerization, thereby providing guidance with enhanced resolution. By fine-tuning parameters, including optical irradiance, exposure time, projection region, step distance, can tailor penetration stemming from neighboring layers, preventing channel blockage due overexposure or compromised bonding stability owing insufficient curing. Remarkably, this allow preparation cross-sectional dimensions 20 μm × using commercial printer pixel size 10 μm; significantly higher than previous reports. In addition, method enable biocompatible drop-maker units be used cell encapsulation. general, current DZC-VPP major advances precise represents significant forward widespread applications microfluidics-based biomedical fields.

Language: Английский

Citations

14
Considerations about Cytotoxicity of Resin-Based Composite Dental Materials: A Systematic Review DOI Open Access

Kacper Wiertelak-Makała,

Izabela Szymczak-Pajor, Kinga Bociong

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 152 - 152

Published: Dec. 21, 2023

The dental material industry is rapidly developing resin-based composites (RBCs), which find widespread use in a variety of clinical settings. As such, their biocompatibility has gained increasing interest. This literature review presents summary research into the cytotoxicity methacrylate-based published from 2017 to 2023. Subject analysis were 14 vitro studies on human and murine cell lines. Cytotoxicity included was measured via MTT assay, LDH WST-1 assay. QUIN Risk Bias Tool performed validate studies. Included (based entirely results studies) provide evidence dose- time-dependent composites. Oxidative stress depletion cellular glutathione (GSH) suggested as reasons for cytotoxicity. Induction apoptosis by RBCs indicated. While remain golden standard restorative materials, potential cannot be ignored due direct long-term exposure. Further investigations trials are required understand molecular mechanism produce novel materials with improved safety profiles.

Language: Английский

Citations

12
Different Polymers for the Base of Removable Dentures? Part II: A Narrative Review of the Dynamics of Microbial Plaque Formation on Dentures DOI Open Access
Pierre Le Bars, Alain Ayepa Kouadio,

Yves Amouriq

et al.

Polymers, Journal Year: 2023, Volume and Issue: 16(1), P. 40 - 40

Published: Dec. 21, 2023

This review focuses on the current disparities and gaps in research characteristics of oral ecosystem denture wearers, making a unique contribution to literature this topic. We aimed synthesize state knowledge concerning biological behavior different polymers used prosthetics. Whichever polymer is composition prosthetic base (poly methyl methacrylate acrylic (PMMA), polyamide (PA), or polyether ether ketone (PEEK)), simple presence removable prosthesis cavity can disturb balance microbiota. phenomenon aggravated by poor hygiene, resulting an increased microbial load coupled with reduced salivation that associated older patients. In 15–70% patients, imbalance leads appearance inflammation under (denture stomatitis, DS). DS dependent equilibrium—as well as reciprocal, fragile, constantly dynamic conditions—between host microbiome cavity. Several local general parameters contribute balance. Locally, formation plaque dentures (DMP) depends phenomena adhesion, aggregation, accumulation microorganisms. To limit DMP, apart from lifestyle must be polished regularly immersed disinfectant bath. It also covered insulating coating. long term, relining maintenance established control proliferation. On other hand, several conditions specific (aging; heredity; allergies; diseases such diabetes mellitus cardiovascular, respiratory, digestive diseases; immunodeficiencies) make management difficult. Thus, second part addresses complexity DMP depending used. The methodology followed comprised formulation search strategy, definition inclusion exclusion criteria, selection studies for analysis. PubMed database was searched independently pertinent studies. A total 213 titles were retrieved electronic databases, after applying we selected 84 articles possible interactions between environment, particular emphasis Candida albicans.

Language: Английский

Citations

8
The Cytotoxic Effect of Thermoplastic Denture Base Resins: A Systematic Review DOI Creative Commons

Shankargouda Patil,

Frank W. Licari, Shilpa Bhandi

et al.

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(8), P. 411 - 411

Published: Aug. 3, 2023

Partial or complete dentures are constructed from thermoplastic resins that thermally processed and molded. This review examines the presently available evidence for cytotoxicity of thermoplasticized denture base on human gingival epithelial cells, adipose fibroblasts; amnion mouse fibroblasts. Electronic searches were performed PubMed, Scopus, Web Science, Google Scholar databases to identify relevant articles be included in until September 2022. Clinical, vivo, vitro studies English language searched for. The quality was assessed using Toxicological data Reliability Assessment tool (ToxRTool) developed by European Commission's Joint Research Centre. GRADE assessment used evaluate certainty evidence. Seven review. overall risk bias determined high, with majority found reliable restrictions not reliable. Only two considered without based ToxRTool assessment. effect viability cell adherence fibroblasts (L929s) is significant. Conditioned media unpolished specimens significantly more toxic cultured cells than those polished specimens. may concern cases poor post-processing dentures. Based limited available, there low-certainty appear biocompatible show insignificant cytotoxicity. Further well-designed trials adhering standard reporting guidelines objective measures necessary before outlining universal best practice. Long-term vivo clinical corroborate laboratory findings outcomes. Denture constant contact oral tissues, cytotoxic components released irritate inflame tissues provoke an allergic response.

Language: Английский

Citations

4
Oxidative damage analysis and cell viability of Drosophila melanogaster exposed to three different endodontic sealers: an in vivo and ex vivo study DOI
Cristiana Pereira Malta, Elize Aparecida Santos Musachio, Eliana Jardim Fernandes

et al.

Odontology, Journal Year: 2024, Volume and Issue: 112(4), P. 1178 - 1185

Published: March 17, 2024

Language: Английский

Citations

1
Digital Light Processing 3D Printing for Microfluidic Chips with Enhanced-resolution via Dosing- and Zoning-controlled vat Photopolymerization DOI Creative Commons
Huanan Wang,

Zhiming Luo,

Haoyue Zhang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 4, 2023

Abstract Conventional manufacturing techniques to fabricate microfluidic chips like soft lithography and hot embossing processes show limitations including difficulty in preparing multiple-layered structures, cost- labor-consuming fabrication process, low productivity. Digital light processing (DLP) technology has recently emerged as a cost-efficient microfabrication approach for 3D printing of chips, wherein, however, the resolution microchannel is still limited sub-100 microns at best. We hereby developed an innovative DLP strategy high-resolution scalable via dosing- zoning-controlled vat photopolymerization (DZC-VPP). Specifically, we proposed modified mathematical model precisely predict accumulated UV irradiance resin photopolymerization, thereby providing guidance with enhanced resolution. By fine-tuning parameters optical irradiance, exposure time, projection region step distance, can tailor penetration stemming from neighboring layers, therefore avoiding channel blockage due over-exposure or compromised bonding stability owing insufficient curing. Remarkably, this enable biocompatible drop-makers that be used cell encapsulation. In general, current DZC-VPP method major advances precise fabrication, represents significant forward widespread applications microfluidics-based biomedical fields.

Language: Английский

Citations

1