Bioinformatics Analysis and Experimental Validation of Epigallocatechin-3-gallate Against Iopromide-induced Injury in HEK-293 CellsviaAnti-oxidative and Anti-inflammation Pathways DOI Open Access

Yuh‐Feng Tsai,

Chia-Wen Tsai, Jai‐Sing Yang

et al.

In Vivo, Journal Year: 2024, Volume and Issue: 38(6), P. 2617 - 2628

Published: Oct. 29, 2024

Background/Aim: The administration of contrast agents can adversely affect kidney function. Nevertheless, the nephrotoxicity iopromide in human renal cells, potential therapeutic agents, and underlying molecular mechanisms have not been thoroughly investigated. Materials Methods: proliferation HEK-293 cells was assessed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay. Apoptotic cell death examined TUNEL assay caspase-3 activity measurements. impacts pathways epigallocatechin-3-gallate (EGCG) on iopromide-induced damage were analyzed through whole transcriptome sequencing. redox state by measuring reactive oxygen species (ROS) production 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Results: Iopromide-induced inhibition apoptosis counteracted EGCG co-treatment. Pathway analysis revealed that molecules related to antioxidant anti-inflammatory responses, such as ERK1/2, STAT1, NF-B, pivotal action EGCG. Conclusion: ROS production, decreased DPPH ability, DNA strand breaks, elevated activity, reduced all reversed co-treatment cells. likely involve attenuation oxidative stress, inflammatory apoptosis, with regulation NF-B pathways. Further research is necessary confirm protective effects function, particularly against induced like iopromide.

Language: Английский

Biosynthesis of titanium dioxide nanoparticles using peel extract of Parkia speciosa for methyl orange degradation DOI

Khoiriah Khoiriah,

Reza Audina Putri

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 170, P. 120 - 129

Published: May 17, 2024

Language: Английский

Citations

6
Synergetic comparative study: Photocatalytic and biological investigations of green-synthesized metal oxide nanoparticles DOI

Rabiya Kousar,

Zia Ul Haq Khan, Sana Sabahat

et al.

Nano-Structures & Nano-Objects, Journal Year: 2024, Volume and Issue: 38, P. 101184 - 101184

Published: May 1, 2024

Language: Английский

Citations

4
Review on titanium oxide (TiO2) nanomaterials in multidomain investigations DOI

Shivani,

Ramesh S. Bhat,

A.G. Bindu

et al.

Nano-Structures & Nano-Objects, Journal Year: 2025, Volume and Issue: 41, P. 101455 - 101455

Published: Feb. 1, 2025

Language: Английский

Citations

0
Sol–Gel Synthesis of TiO2 with Pectin and Their Efficiency in Solar Cells Sensitized by Quantum Dots DOI Creative Commons
Jean Flores‐Gómez, Silvia Elena Mota Macías,

Juan P. Guerrero-Jiménez

et al.

Gels, Journal Year: 2024, Volume and Issue: 10(7), P. 470 - 470

Published: July 17, 2024

In this study, titanium oxide TiO

Language: Английский

Citations

1
Bioinformatics Analysis and Experimental Validation of Epigallocatechin-3-gallate Against Iopromide-induced Injury in HEK-293 CellsviaAnti-oxidative and Anti-inflammation Pathways DOI Open Access

Yuh‐Feng Tsai,

Chia-Wen Tsai, Jai‐Sing Yang

et al.

In Vivo, Journal Year: 2024, Volume and Issue: 38(6), P. 2617 - 2628

Published: Oct. 29, 2024

Background/Aim: The administration of contrast agents can adversely affect kidney function. Nevertheless, the nephrotoxicity iopromide in human renal cells, potential therapeutic agents, and underlying molecular mechanisms have not been thoroughly investigated. Materials Methods: proliferation HEK-293 cells was assessed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay. Apoptotic cell death examined TUNEL assay caspase-3 activity measurements. impacts pathways epigallocatechin-3-gallate (EGCG) on iopromide-induced damage were analyzed through whole transcriptome sequencing. redox state by measuring reactive oxygen species (ROS) production 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Results: Iopromide-induced inhibition apoptosis counteracted EGCG co-treatment. Pathway analysis revealed that molecules related to antioxidant anti-inflammatory responses, such as ERK1/2, STAT1, NF-B, pivotal action EGCG. Conclusion: ROS production, decreased DPPH ability, DNA strand breaks, elevated activity, reduced all reversed co-treatment cells. likely involve attenuation oxidative stress, inflammatory apoptosis, with regulation NF-B pathways. Further research is necessary confirm protective effects function, particularly against induced like iopromide.

Language: Английский

Citations

0