The Impact of Using Laser and Milling Techniques to Create Zirconia Patterns on Streptococcus oralis Biofilm Formation DOI Creative Commons
Neusa Silva, Joana Marques, João Caramês

et al.

Ceramics, Journal Year: 2024, Volume and Issue: 7(4), P. 1855 - 1866

Published: Dec. 3, 2024

This study aimed to evaluate zirconia dental implant surfaces patterned using Nd:YAG laser or conventional milling techniques against Streptococcus oralis adhesion and biofilm formation. Zirconia discs were subjected surface patterning treatments categorized into four groups: groove texturing by (GM), pore (PM), (GL), Nd: YAG (PL). CECT 907T was cultivated on enriched blood agar plates then transferred a brain–heart infusion modified medium incubated at 37 °C under anaerobic conditions until reaching the exponential growth phase. The bacterial suspension seeded 24-well containing treated discs. viability of bacteria within determined based colony-forming unit (CFU) counts, while total quantified measuring its biomass. A qualitative analysis conducted scanning electron microscopy (SEM) images morphology. statistical multigroup comparisons performed Kruskal–Wallis test with post hoc pairwise comparison, as well Mann Whiney U test, significance set p < 0.05. After both 1 h 24 incubation discs, all groups showed similar results, no statistically significant differences (p > 0.05). comparison laser-treated conventionally milled surfaces, grooves versus pores for CFU also revealed 0.05) culture. Biomass quantification 24-h time points results across groups, without differences. When comparing machined in terms biomass, observed Similarly, between groove-patterned pore-patterned difference. patterns did not change formation behavior. Additional studies are recommended expand our knowledge this area.

Language: Английский

The Impact of Using Laser and Milling Techniques to Create Zirconia Patterns on Streptococcus oralis Biofilm Formation DOI Creative Commons
Neusa Silva, Joana Marques, João Caramês

et al.

Ceramics, Journal Year: 2024, Volume and Issue: 7(4), P. 1855 - 1866

Published: Dec. 3, 2024

This study aimed to evaluate zirconia dental implant surfaces patterned using Nd:YAG laser or conventional milling techniques against Streptococcus oralis adhesion and biofilm formation. Zirconia discs were subjected surface patterning treatments categorized into four groups: groove texturing by (GM), pore (PM), (GL), Nd: YAG (PL). CECT 907T was cultivated on enriched blood agar plates then transferred a brain–heart infusion modified medium incubated at 37 °C under anaerobic conditions until reaching the exponential growth phase. The bacterial suspension seeded 24-well containing treated discs. viability of bacteria within determined based colony-forming unit (CFU) counts, while total quantified measuring its biomass. A qualitative analysis conducted scanning electron microscopy (SEM) images morphology. statistical multigroup comparisons performed Kruskal–Wallis test with post hoc pairwise comparison, as well Mann Whiney U test, significance set p < 0.05. After both 1 h 24 incubation discs, all groups showed similar results, no statistically significant differences (p > 0.05). comparison laser-treated conventionally milled surfaces, grooves versus pores for CFU also revealed 0.05) culture. Biomass quantification 24-h time points results across groups, without differences. When comparing machined in terms biomass, observed Similarly, between groove-patterned pore-patterned difference. patterns did not change formation behavior. Additional studies are recommended expand our knowledge this area.

Language: Английский

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