Electrospun Scaffolds of Polylactic Acid, Collagen, and Amorphous Calcium Phosphate for Bone Repair

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(11), P. 2529 - 2529

Published: Oct. 25, 2023

Most electrospun scaffolds for bone tissue engineering typically use hydroxyapatite (HA) or beta tricalcium phosphate (β-TCP). However, the biological activity of these crystalline compounds can be limited due to their low solubility. Therefore, amorphous calcium (ACP) may an alternative in repair scaffolds. This study analyzes morphology, porosity, mechanical strength, and surface chemistry composed polylactic acid collagen integrated with (MHAP) (MACP). In addition, vitro biocompatibility, osteogenic differentiation, growth factor production associated using human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) are evaluated. The results show that MHAP MACP exhibit a fibrous morphology interconnected pores. Both favorable biocompatibility stimulate proliferation osteogenesis hWJ-MSCs. cell adhesion osteocalcin greater scaffold compared scaffold. shows significant bone-repair-related factors such as transforming factor-beta 1 (TGF-β1), providing solid basis its engineering.

Language: Английский

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Preparation, design, and characterization of an electrospun polyurethane/calcium chloride nanocomposite scaffold with improved properties for skin tissue regeneration

Journal of Industrial Textiles, Journal Year: 2024, Volume and Issue: 54

Published: Jan. 1, 2024

The present research paper explores the potential of electrospun nanofibers in promising field skin tissue engineering. Specifically, we propose an advanced preparation and characterization Polyurethane/Calcium Chloride (PU/CaCl 2 ) nanocomposite scaffold, devised to boost scaffold’s physicochemical biological properties for regeneration. By incorporating CaCl into PU matrix using electrospinning process, were able fabricate a novel scaffold. morphological examination through Field Emission Scanning Electron Microscope (FESEM) revealed that fiber diameter PU/CaCl2 (563 ± 147 nm) scaffold was notably smaller compared control (784 149 nm). presence corroborated by Fourier-Transform Infrared Spectroscopy (FTIR) Thermogravimetric Analysis (TGA). Furthermore, PU/CaCl exhibited superior tensile strength (10.81 MPa) over pristine (Tensile −6.16 MPa, Contact angle - 109° 1° Roughness 854 32 enhanced wettability (72° 2°) reduced surface roughness (274 104 nm), as verified Atomic Force Microscopy. developed demonstrated improved anticoagulant properties, indicating its successful integration within environment. significant advancement polymer nanofibers, offering breakthrough However, additional studies are required thoroughly evaluate effectiveness promoting cell adhesion, proliferation, differentiation. We aim catalyze advancements revealing creation potent leveraging nanofibers. Encouraging deeper exploration this innovative composite engineering, stands foundation pioneering more innovative, efficient, sustainable solutions biomedical applications.

Language: Английский

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Fabrication of a tissue engineering mat by emulsion‐based electrospinning of polycaprolactone/silk fibroin using a new solvent system

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(5)

Published: May 1, 2024

Abstract The development of electrospun tissue engineering scaffolds using silk fibroin and polycaprolactone (PCL) has been reported. challenge in this system is the design a suitable solvent with high stability. In study, mat was PCL‐fibroin fibers an emulsion consisting PCL blend polyethylene oxide (PEO). effects different concentrations PEO ratios formic acid: distilled water (FA:DW, for PEO) chloroform: acid (Chl:FA, PCL) were studied. FA:DW Chl:FA 70:30 proved to make optimized stable system. addition, by controlling absorption mat, we its weight loss maintained integrity up 14 days. Also, mechanical cell attachment properties as expected. Chorioallantoic membrane (CAM) assay exhibited potential mats induce angiogenesis. Furthermore, subcutaneous implantation mouse model elicited no significant inflammatory response. This study provides simple promising electrospinning fabrication applications.

Language: Английский

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Enzymatically Degradable Electrospun Nanogel Scaffolds Promoted in Vitro Proliferation of rADSC

ACS Applied Polymer Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Scaffolds that mimic the extracellular matrix (ECM) can promote proliferation of stem cells. Poly(N-isopropylacrylamide) (PNIPAM)/gelatin (PG) nanogel electrospun membranes were designed to replicate hierarchical and dynamic nature ECMs. First, PG nanogels with semi-interpenetrating network structures synthesized by emulsion polymerization. Characterization using 1H NMR FTIR spectroscopies confirmed successful incorporation gelatin into PNIPAM-based network. The spherical a size 184.1–231.9 nm, they demonstrated thermoresponsive behavior. rehydrated nanofibers exhibited an average diameter 663.6 ± 50.1 nm Young's modulus 1.3 0.1 MPa at 37 °C. membrane completely degraded within 10 min collagenase treatment. Adipose-derived cells from rats good on membranes, cell in PNI50 group being 1.47 times tissue culture plate (TCP) group. showed potential for expansion.

Language: Английский

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Hydrogels, Scaffolds, Kartogenin for Regenerative Medicine

Theoretical and Natural Science, Journal Year: 2025, Volume and Issue: 72(1), P. 170 - 176

Published: Jan. 7, 2025

Hydrogels, scaffolds and kartogenin (KGN) play an important role in tissue engineering regenerative medicine. Hydrogels with water retention properties provide a biocompatible environment that promotes cell growth differentiation. Scaffolds designed to mimic the extracellular matrix can promote adhesion formation. Kartogenin induces differentiation of mesenchymal stem cells into chondrocytes, thereby promoting cartilage The combination these three components represents promising strategy for repair, hydrogel providing necessary moisture, scaffold structural support, regeneration

Language: Английский

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Influence of Different Solvents on the Mechanical Properties of Electrospun Scaffolds

Materials, Journal Year: 2025, Volume and Issue: 18(2), P. 355 - 355

Published: Jan. 14, 2025

This article investigates the influence of different solvents on mechanical properties biocompatible and biodegradable polycaprolactone (PCL) scaffolds. During research, using electrospinning technology, 27 samples nanofibers exposed to were produced. A tensile test was performed produced nanofiber samples, properties, yield strength, elastic modulus, elongation calculated, load–displacement stress–strain dependence diagrams compared from obtained results. The strongest singled out, its with those biological tissues application in tissue engineering. structure determined a scanning electron microscope, structures compared. After calculating nanofibers, identified, PCL chloroform, which has an modulus 9.86 MPa strength 1.11 ± 0.32 MPa. type solvent used production solution affects homogeneity fibre shape filaments. In lower viscosity, filaments are more homogeneous evenly distributed.

Language: Английский

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Multifunctional Biological Performance of Electrospun PCL Scaffolds Formulated with Silver Sulfide Nanoparticles

Polymers, Journal Year: 2025, Volume and Issue: 17(2), P. 230 - 230

Published: Jan. 17, 2025

Our work describes the green synthesis of silver sulfide nanoparticles (Ag2S NPs) and their formulation into polycaprolactone fibers (PCL), aiming to improve multifunctional biological performance PCL membranes as scaffolds. For this purpose, an extract rosemary (Salvia rosmarinus) was employed a reducing agent for Ag2S NPs, obtaining irregular NPs clusters 5-60 nm, with characteristic SPR absorption at 369 nm. successfully incorporated by electrospinning using heparin (HEP) stabilizer/biocompatibility agent, nanostructured ca. 500-800 nm diameter. Different amounts (0.05, 0.5, 1 wt.%) enhanced membranes' surface polarity mechanical performance, controlled ion release after 6 days submerged in PBS solution, determined cyclic voltammetry. As result, PCL/HEP/Ag2S scaffolds exhibit high antibacterial (80-90%) early stages contact (3 h) against E. coli S. aureus. Also, cytotoxicity analysis demonstrated that are biocompatible fibroblast cell regeneration, which is optimal application To validate regenerative response scaffolds, wounds were induced Wistar rats, presenting favorable healing 1%, compared untreated wound. results indicated enable development novel nanomaterials performance.

Language: Английский

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Bioadhesive levan-based coaxial nanofibrous membranes with enhanced cell adhesion and mesenchymal stem cell differentiation

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: unknown, P. 123337 - 123337

Published: Jan. 1, 2025

Language: Английский

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Advances in tissue engineering of peripheral nerve and tissue innervation – a systematic review

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

Although various options are available to treat injured organs and peripheral nerves, none is without limitations. Auto- allografts the first choice of treatment, but tissue survival or functionality not guaranteed due often limited vascular neural networks. In response, tissue-engineered solutions have been developed, yet clinical translations rare. this study, a systematic review was performed on advancements for nerves tissues, aid future developments in bridging gap toward clinic by identifying high-potential unexplored areas. A search PubMed, Embase, Web Science, Scopus until November 9, 2023. Search terms involved “tissue engineering,” “guided,” scaffold,” graft,” together with “innervation” “reinnervation.” Original vivo vitro studies meeting inclusion criteria (tissue-engineered nerve/innervation tissue) no exclusion (no full text available; written foreign language; nonoriginal article; tissue-engineering central nervous system; publication before 2012; insufficient study quality reproducibility) were assessed. total 68 out 3626 original included. Data extraction based disease model, cell origin host species, biomaterial nature composition, external stimuli biological, chemical physical origin. engineering still its infancy, explored innervation strategies today highlighted respect biomaterials, types, stimuli. The findings emphasize that natural pre-seeding autologous sources, reproductive beneficial research. Natural biomaterials possess important cues required cell-material interaction closely resemble native biomechanical, geometrical composition. Autologous cells induce functionalization. As these pose risk immunorejection demonstrated good outcomes, they most likely fulfill demands.

Language: Английский

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Nanofibrous Scaffolds’ Ability to Induce Mesenchymal Stem Cell Differentiation for Soft Tissue Regenerative Applications

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 239 - 239

Published: Feb. 11, 2025

Mesenchymal stem cells (MSCs) have gained recognition as a highly versatile and promising cell source for repopulating bioengineered scaffolds due to their inherent capacity differentiate into multiple types. However, MSC implantation techniques often yielded inconsistent clinical results, underscoring the need advanced approaches enhance therapeutic efficacy. Recent developments in three-dimensional (3D) provided significant breakthrough by closely mimicking vivo environment, addressing limitations of traditional two-dimensional (2D) cultures. Among these, nanofibrous proven particularly effective, offering an optimal 3D framework, growth-permissive substrates, delivery trophic factors crucial survival regeneration. Furthermore, selection appropriate biomaterials can amplify paracrine effects MSCs, promoting both proliferation targeted differentiation. The synergistic combination MSCs with has demonstrated remarkable potential achieving repair, regeneration, tissue-specific differentiation enhanced safety efficacy, paving way routine applications. In this review, we examine most recent studies (2013–2023) that explore combined use cardiogenic, epithelial, myogenic, tendon, vascular lineages. Using PubMed, identified analyzed 275 relevant articles based on search terms “Nanofibers”, “Electrospinning”, “Mesenchymal cells”, “Differentiation”. This review highlights critical advancements platform tissue By summarizing key findings from last decade, it provides valuable insights researchers clinicians aiming optimize scaffold design, integration, translational These could significantly influence future research directions development more effective regenerative therapies.

Language: Английский

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