Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds DOI Creative Commons
Marcelo Marucci Pereira Tangerina, Luciana Costa Furtado, Vida M. B. Leite

et al.

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(12), P. e0244385 - e0244385

Published: Dec. 21, 2020

Resorting to a One Strain Many Compounds (OSMAC) approach, the marine Streptomyces sp. BRB081 strain was grown in six different media settings over 1, 2, 3 or 7 days. Extractions of mycelium and broth were conducted separately for each cultivation period by sonication using methanol/acetone 1:1 agitation with ethyl acetate, respectively. All acetate crude extracts analysed HPLC-MS/MS data treatment performed through GNPS platform MZmine 2 software. In parallel, genome sequenced, assembled mined search biosynthetic gene clusters (BGC) secondary metabolites AntiSMASH 5.0 Spectral library tool allowed annotation desferrioxamines, fatty acid amides, diketopiperazines, xanthurenic and, remarkably, cyclic octapeptides surugamides. Molecular network analysis observation surugamides cluster, where surugamide A protonated molecule corresponding B-E isomers, as well two potentially new analogues, detected. Data software distinguish that largest amount obtained cultivating SCB medium during days extraction culture broth. Using same treatment, chemical barcode created easy visualization comparison produced overtime all media. By mining four regions detected supporting metabolic data. Cytotoxic evaluation MTT assay revealed highest bioactivity also observed optimal conditions those production, suggesting these be main active compounds herein. This method identification guided selection best production bioactive compounds.

Language: Английский

Advances in microbial culturing conditions to activate silent biosynthetic gene clusters for novel metabolite production DOI Open Access

Hailey Tomm,

Lorena Ucciferri,

Avena C. Ross

et al.

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2019, Volume and Issue: 46(9-10), P. 1381 - 1400

Published: June 8, 2019

Language: Английский

Citations

63
Marine Fungi from the Sponge Grantia compressa: Biodiversity, Chemodiversity, and Biotechnological Potential DOI Creative Commons
Elena Bovio, Laura Garzoli, A. Poli

et al.

Marine Drugs, Journal Year: 2019, Volume and Issue: 17(4), P. 220 - 220

Published: April 11, 2019

The emergence of antibiotic resistance and viruses with high epidemic potential made unexplored marine environments an appealing target source for new metabolites. Marine fungi represent one the most suitable sources discovery compounds. Thus, aim this work was (i) to isolate identify associated Atlantic sponge

Language: Английский

Citations

61
The Biosynthesis of Fungal Secondary Metabolites: From Fundamentals to Biotechnological Applications DOI

Olga V. Mosunova,

Jorge C. Navarro-Muñoz, Jérôme Collemare

et al.

Elsevier eBooks, Journal Year: 2020, Volume and Issue: unknown, P. 458 - 476

Published: Feb. 24, 2020

Language: Английский

Citations

57
Discovery of novel secondary metabolites encoded in actinomycete genomes through coculture DOI Creative Commons
Ji Hun Kim, Namil Lee, Soonkyu Hwang

et al.

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2021, Volume and Issue: 48(3-4)

Published: Jan. 1, 2021

Abstract Actinomycetes are a rich source of bioactive natural products important for novel drug leads. Recent genome mining approaches have revealed an enormous number secondary metabolite biosynthetic gene clusters (smBGCs) in actinomycetes. However, under standard laboratory culture conditions, many smBGCs silent or cryptic. To activate these dormant smBGCs, several approaches, including culture-based genetic engineering-based strategies, been developed. Above all, coculture is promising approach to induce production from actinomycetes by mimicking ecological habitat where cryptic may be activated. In this review, we introduce studies that aim expand the chemical diversity actinomycetes, categorizing cases type partner. Furthermore, discuss current challenges need overcome support elicitation compounds

Language: Английский

Citations

53
Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds DOI Creative Commons
Marcelo Marucci Pereira Tangerina, Luciana Costa Furtado, Vida M. B. Leite

et al.

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(12), P. e0244385 - e0244385

Published: Dec. 21, 2020

Resorting to a One Strain Many Compounds (OSMAC) approach, the marine Streptomyces sp. BRB081 strain was grown in six different media settings over 1, 2, 3 or 7 days. Extractions of mycelium and broth were conducted separately for each cultivation period by sonication using methanol/acetone 1:1 agitation with ethyl acetate, respectively. All acetate crude extracts analysed HPLC-MS/MS data treatment performed through GNPS platform MZmine 2 software. In parallel, genome sequenced, assembled mined search biosynthetic gene clusters (BGC) secondary metabolites AntiSMASH 5.0 Spectral library tool allowed annotation desferrioxamines, fatty acid amides, diketopiperazines, xanthurenic and, remarkably, cyclic octapeptides surugamides. Molecular network analysis observation surugamides cluster, where surugamide A protonated molecule corresponding B-E isomers, as well two potentially new analogues, detected. Data software distinguish that largest amount obtained cultivating SCB medium during days extraction culture broth. Using same treatment, chemical barcode created easy visualization comparison produced overtime all media. By mining four regions detected supporting metabolic data. Cytotoxic evaluation MTT assay revealed highest bioactivity also observed optimal conditions those production, suggesting these be main active compounds herein. This method identification guided selection best production bioactive compounds.

Language: Английский

Citations

52