Docosahexaenoic Acid-Loaded Nanostructured Lipid Carriers for the Treatment of Peri-Implantitis in Rats

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 1872 - 1872

Published: Jan. 18, 2023

Being the most common cause of implant failure, peri-implantitis is defined as a pathological condition associated with occurrence peri-implant plaque, characterized by mucosal inflammation and progressive loss supporting bone tissue attributed to persistence pro-inflammatory cytokines. Docosahexaenoic acid (DHA), which type omega-3 polyunsaturated fatty acid, generally used for treatment many inflammatory diseases. However, suitable form dosing its therapeutic effect on remain unclear. In this study, novel nanostructured lipid carrier (NLC) loaded squalene DHA was fabricated (DHA-loaded NLC). The encapsulation efficiency drug loading values DHA-loaded NLC were 78.13% ± 1.85% 28.09% 0.48%, respectively. release gradual steady until 144 h. addition, free-radical-scavenging rate (0.57 0.03) much higher than that sole (0.17 0.003). By inhibiting nuclear factor-κB p65 translocation, prevented activation downstream pathways exerted anti-inflammatory effects macrophages. Moreover, showed better preventing alveolar resorption rat model DHA. Hence, enhanced bioavailability DHA, offering approach peri-implantitis.

Language: Английский

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Impact of the microbiome on colorectal cancer development

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 29 - 72

Published: Jan. 1, 2024

Language: Английский

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New Forms of Neuroactive Phospholipids for DHA Enrichment in Brain

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(3), P. 116 - 116

Published: Feb. 29, 2024

Low levels of docosahexaenoic acid (DHA) in the brain have been related to neurological disorders, like Alzheimer’s disease (AD). After ingestion, dietary DHA must cross blood–brain barrier, where it is absorbed as lysophosphatidylcholine (LPC), due its role a preferential carrier brain. This work aimed at production LPC-DHA extracts be used supplementation/food fortification intended neural enrichment DHA. As rich DHA, especially phospholipids (PL), Atlantic mackerel (Scomber scombrus, caught Spring/2022) was raw material. The polar lipids fraction separated and hydrolysed with Rhizomucor miehei lipase, enzymatically convert phosphatidylcholine (PC) into LPC. fish (muscle by-products) for total (TL) content, lipid classes (LC) fatty (FA) profile evaluation, whilst were studied LC FA analysis. Muscle TL ranged between 1.45 4.64 g/100 g (WW), while by-products accounted 7.56-8.96 g, highest contents being found March. However, PL more abundant muscle (22.46–32.20% TL). For extracts, represented 50.79% TL, among which PC corresponded 57.76% phosphatidylethanolamine 42.24%. hydrolysis, nearly half this converted When compared initial PC, relative content (33.6% FA) significantly higher after hydrolysis: 55.6% 73.6% Such extract, obtained from undervalued species, may represent promising strategy increase uptake cells allowing species upgrade.

Language: Английский

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Omega 3 Fatty Acid as a Health Supplement: An Overview of its Manufacture and Regulatory Aspects

Current Research in Nutrition and Food Science Journal, Journal Year: 2024, Volume and Issue: 12(1), P. 70 - 90

Published: April 25, 2024

Dietary supplements are used for potential health benefits and mainly intended to provide those nutrients that may be insufficiently consumed through regular dietary intake. They mostly made from natural sources readily available in the form of capsules, tablets, or liquid form. Nowadays, omega-3 fatty acids (n−3 FAs) high demand have gained noteworthy popularity as human body cannot produce them, need administrated externally. polyunsaturated (PUFA) characterized by at least one cis double bond third fourth omega-end carbons, with primary examples being ɑ- linolenic acid (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA) classified functional foods. The these include aquatic organisms like freshwater marine fish, microalgae, seaweeds; nuts seeds plant oils, fortified n-3 FAs, biologically active molecules, highly susceptible oxidation due bonds their long chains, leading degradation over time, during storage. Various encapsulation systems, such gels, emulsions, powders, been developed effectively encapsulate PUFAs enhance chemical stability, dispersibility, bioavailability. These quality control measures essential guarantee effectiveness safety products containing FAs. This paper explores various summarizes multiple manufacturing techniques production products. Additionally, article tried correlate aspects regulations an attempt prevent exposure consumers harmful ingredients contained within Lastly, recommendations harmonizing regulation proposed.

Language: Английский

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Thraustochytrids and Algae as Sustainable Sources of Long-Chain Omega-3 Fatty Acids for Aquafeeds

Sustainability, Journal Year: 2024, Volume and Issue: 16(21), P. 9142 - 9142

Published: Oct. 22, 2024

There is limited ability to biosynthesize long-chain omega-3 fatty acids such as EPA and DHA in food webs leading humans. Seafood the key source of ω3 LC-PUFA, with aquaculture expected meet rising global demand; however, marine fish have a high dietary requirement for themselves. This was traditionally met using unsustainable oil meal, but supply environmental concerns dictated research on replacements. Among industrial sources DHA, microalgae especially thraustochytrids stand out resources concentrations. Although unicellular, are not they photosynthetic instead microheterotrophs. removes light facilitates yields monoculture production single-cell oils. The availability, concentrations, usually one or other essential acid permits calibration dose, which useful their effects mainly been considered together medical research. different cell function precursors bioactive compounds. Using thraustochytrids, microalgae, heterotrophic dinoflagellates, importance has investigated. optimizing growth early life stages scallops finfish preferentially incorporated into membrane phospholipids. microheterotrophs can contribute treatment wastewater waste gas, further enhancing sustainability reducing costs aquaculture.

Language: Английский

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Marine Animal Co-Products – How Improving Their Use as Rich Sources of Health-Promoting Lipids Can Foster Sustainability

Published: Dec. 12, 2023

Marine lipids are recognized for their health promoting features, mainly being the primary sources of omega-3 fatty acids, and therefore critical human nutrition in an age when global supply these nutrients is experiencing unprecedent pressure due to ever-increasing demand. The seafood industry originates a considerable yield co-products worldwide that, while already explored other purposes, remain mostly undervalued as sustainable healthy lipids, often low value oil production. These especially appealing lipid since, besides well-known nutritional upside marine animal fat, particularly rich polyunsaturated acids (PUFAs), they have also interesting bioactive properties, which may garner them further interest, not only food, but high-end applications. Besides added that represent valuable sources, there obvious ecological reducing waste. In this sense, repurposing bioresources will contribute more use strain on heavily depleted stocks. Therefore, untapping potential aligns with both environmental goals by guaranteeing additional eco-conscious practices.

Language: Английский

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Omega-3 PUFAs as a Dietary Supplement in Senile Systemic Amyloidosis

Nutrients, Journal Year: 2023, Volume and Issue: 15(3), P. 749 - 749

Published: Feb. 1, 2023

Eicosapentaenoic acid (EPA; 20:5) and docosahexaenoic (DHA; 22:6), two omega-3 poly-unsaturated fatty acids (PUFAs), are the main components in oil derived from fish other marine organisms. EPA DHA commercially available as dietary supplements considered to be very safe contribute guaranteeing human health. Studies report that PUFAs have a role contrasting neurodegenerative processes related amyloidogenic proteins, such β-amyloid for AD, α-synuclein PD, transthyretin (TTR) TTR amyloidosis. In this context, we investigated if can interact directly with TTR, binding inside thyroxin-binding pockets (T4BP) tetramer stabilization. The data obtained showed stabilizing through interactions T4BP.

Language: Английский

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Crabs Eriocheir japonica and Paralithodes camtschaticus Are a Rich Source of Lipid Molecular Species with High Nutritional Value

Foods, Journal Year: 2023, Volume and Issue: 12(18), P. 3359 - 3359

Published: Sept. 7, 2023

Due to their valuable meat and hepatopancreas, the world's most famous delicacies, crabs, have become target species of commercial fisheries aquaculture. By methods supercritical fluid high-performance liquid chromatography, coupled with high resolution mass spectrometry, we analyzed triacylglycerols (TG) phospholipids (PL)-glycerophosphoethanolamines (PE), glycerophosphocholines (PC), glycerophosphoserines (PS), glycerophosphoinositols (PI)-in hepatopancreas muscles Japanese mitten crab Eriocheir japonica red king Paralithodes camtschaticus inhabiting Sea Japan. TGs were main class lipids in while they found trace amounts muscle. E. differed from those P. by a higher content 16:0, 16:1, 18:2, 20:4 FA lower eicosapentaenoic docosahexaenoic acids. The molecular acid PC PI; an increased arachidonic PE, PS, PE muscles. nutritional value crabs was confirmed n-3 polyunsaturated fatty data lipid profile provide new background information for future studies on biochemistry aquaculture crabs.

Language: Английский

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Shotgun-Based Mass Spectrometry Analysis of Phospholipid and Triacylglycerol Molecular Species and Eicosanoids in Salmon Muscle Tissue on Feeding Microbial Oil

Marine Drugs, Journal Year: 2023, Volume and Issue: 22(1), P. 11 - 11

Published: Dec. 21, 2023

The continuous growth of aquaculture places a growing demand on alternative sources fish oil (FO). Certain microorganisms provide sustainable replacement for FO due to their content EPA and DHA, which are essential health. Appreciable evidence shows that changes in feeding may alter the nutritional components salmon; however, influence diets lipid species remains unclear. In this study, identification semi-quantification molecular salmon muscle during with microbial (MO) were carried out by focusing triacylglycerol (TAG) diacyl-phospholipid using shotgun-based mass spectrometry analysis. DHA MO diet was efficiently incorporated into phospholipid structures feeding, followed accumulation muscle. elevated level certain EPA-containing TAGs, such as TAG C52:5 (16:0_16:0_20:5) C54:6 (16:0_18:1_20:5), indicating be an excellent source enhancement abundance ω3 lipids. Further, prostaglandins (PGs) PGE2 PGF3α identified quantified first time salmonid tissue.

Language: Английский

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Growth, Fatty Acid Composition and Extracellular Enzyme Production in the Thraustochytrid, Aurantiochytrium Cultured in Molasses-Based Medium

Published: Sept. 18, 2023

The production of oil by thraustochytrids is still costly because its necessary and expensive culture medium. We evaluated the growth, lipid production, fatty acid composition in an inexpensive, molasses-based (M) Newly isolated thraustochytrid strains were identified as members genus Aurantiochytrium based on 18S rRNA gene sequences. Isolated repeatedly cultured M medium for acclimatization to Acclimatized non-acclimatized basal GY cells extracted Folch method, was analyzed gas chromatograph. growth significantly inhibited culturing medium, whereas improved treatment same affected compared some enzymes such leucine valine aryl-amidase suppressed activities recovered treatment. molasses thus process, profiles extracellular also affected.

Language: Английский

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